Western Blot (WB)
(Western blot analysis of TSPAN33 Antibody (C-term) in T47D cell line lysates (35ug/lane). TSPAN33 (arrow) was detected using the purified Pab.)
Rabbit TSPAN33 Polyclonal Antibody | anti-TSPAN33 antibody
TSPAN33 Antibody (C-term)
Gene Names
TSPAN33; PEN; PEN.; TSPAN-33
Reactivity
Human (Predicted Reactivity: Bovine, Mouse)
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TSPAN33; Polyclonal Antibody; TSPAN33 Antibody (C-term); Tetraspanin-33; Tspan-33; Penumbra; hPen; Proerythroblast new membrane; PEN; anti-TSPAN33 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TSPAN33 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 254-283 amino acids from the C-terminal region of human TSPAN33.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
254-283
Sequence Length
283
Applicable Applications for anti-TSPAN33 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
IHC-P~~1:50~100
FC~~1:10~50
Clone Names
RB23231
Function
Plays an important role in normal erythropoiesis (By similarity). It has a role inthe differentiation of erythroid progenitors(By similarity). Regulates maturation and trafficking of the transmembranemetalloprotease ADAM10 (PubMed:26686862). Negatively regulates ligand-induced Notch activityprobably by regulating ADAM10 activity (PubMed:26686862).
Cellular location
Cell membrane; Multi-pass membrane protein
Tissue location
Predominantly expressed in erythroblasts
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of TSPAN33 Antibody (C-term) in T47D cell line lysates (35ug/lane). TSPAN33 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of TSPAN33 Antibody (C-term) in T47D cell line lysates (35ug/lane). TSPAN33 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human colon carcinoma reacted with TSPAN33 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human colon carcinoma reacted with TSPAN33 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(TSPAN33 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(TSPAN33 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-TSPAN33 antibody
TSPAN33 plays an important role in normal erythropoiesis. It has a role in the differentiation of erythroid progenitors.
Product Categories/Family for anti-TSPAN33 antibody
References
Heikens, M.J., et al. Blood 109(8):3244-3252(2007)
Huang, S., et al. Genomics 86(6):674-684(2005)
Chen, Z., et al. Cancer Genet. Cytogenet. 162(2):95-98(2005)
Huang, S., et al. Genomics 86(6):674-684(2005)
Chen, Z., et al. Cancer Genet. Cytogenet. 162(2):95-98(2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31538
NCBI Official Full Name
tetraspanin-33
NCBI Official Synonym Full Names
tetraspanin 33
NCBI Official Symbol
TSPAN33
NCBI Official Synonym Symbols
PEN; PEN.; TSPAN-33
NCBI Protein Information
tetraspanin-33
UniProt Protein Name
Tetraspanin-33
Protein Family
UniProt Gene Name
TSPAN33
UniProt Synonym Gene Names
PEN; Tspan-33; hPen
UniProt Entry Name
TSN33_HUMAN
Uniprot Description
TSPAN33: Plays an important role in normal erythropoiesis. It has a role in the differentiation of erythroid progenitors. Belongs to the tetraspanin (TM4SF) family.
Protein type: Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 7q32.1
Cellular Component: cell surface; plasma membrane; integral to membrane
Molecular Function: enzyme binding
Biological Process: protein maturation
Research Articles on TSPAN33
Similar Products
Product Notes
The TSPAN33 tspan33 (Catalog #AAA9212936) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 254-283. The TSPAN33 Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's TSPAN33 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000IHC-P~~1:50~100
FC~~1:10~50 . Researchers should empirically determine the suitability of the TSPAN33 tspan33 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TSPAN33, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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