Rabbit anti-Human TSN Polyclonal Antibody | anti-TSN antibody

TSN, ID (TSN, Translin, Component 3 of promoter of RISC) (Biotin)

Cat. Num. AAA6359520

• Gene Names: TSN; C3PO; RCHF1; TBRBP; TRSLN; BCLF-1; REHF-1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TSN; Polyclonal Antibody; ID (TSN; Translin; Component 3 of promoter of RISC) (Biotin); Component 3 of promoter of RISC; anti-TSN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TSN antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: TSN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-146 amino acids from the Central region of human TSN.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TSN Antibody (Center) western blot analysis in HL-60,Jurkat,K562 cell line mouse spleen tissue lysates (35ug/lane).This demonstrates the TSN antibody detected the TSN protein (arrow).)

Immunofluorescence (IF)

(Fluorescent confocal image of Hela cell stained with TSN Antibody(Center).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSN primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).TSN immunoreactivity is localized to Nucleus and Cytoplasm significantly.)

Immunohistochemistry (IHC)

(TSN Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TSN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-TSN antibody

This gene encodes a DNA-binding protein which specifically recognizes conserved target sequences at the breakpoint junction of chromosomal translocations. Translin polypeptides form a multimeric structure that is responsible for its DNA-binding activity. Recombination-associated motifs and translin-binding sites are present at recombination hotspots and may serve as indicators of breakpoints in genes which are fused by translocations. These binding activities may play a crucial role in chromosomal translocation in lymphoid neoplasms.

Product Categories/Family for anti-TSN antibody

Antibodies; Proteins

References

Chiaruttini, C., et al. Proc. Natl. Acad. Sci. U.S.A. 106(38):16481-16486(2009). Lasky-Su, J., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 147B (8), 1345-1354 (2008): Sengupta, K., et al. Biochemistry 45(3):861-870(2006). Kaluzhny, D., et al. J. Biomol. Struct. Dyn. 23(3):257-265(2005). Gupta, G.D., et al. FEBS Lett. 579(14):3141-3146(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 4759270
• NCBI GeneID: 7247
• NCBI Accession #: NP_004613.1
• NCBI GenBank Nucleotide #: NM_004622.2
• UniProt Accession #: Q15631; Q5U0K7; B7Z3X8
• Molecular Weight: 17,279 Da
• NCBI Official Full Name: translin isoform 1
• NCBI Official Synonym Full Names: translin
• NCBI Official Symbol: TSN
• NCBI Official Synonym Symbols: C3PO; RCHF1; TBRBP; TRSLN; BCLF-1; REHF-1
• NCBI Protein Information: translin; component 3 of promoter of RISC; testis brain-RNA binding protein; recombination hotspot-binding protein; recombination hotspot associated factor
• UniProt Protein Name: Translin
• Protein Family: Translin
• UniProt Gene Name: TSN
• UniProt Synonym Gene Names: C3PO
• UniProt Entry Name: TSN_HUMAN

NCBI Description

This gene encodes a DNA-binding protein which specifically recognizes conserved target sequences at the breakpoint junction of chromosomal translocations. Translin polypeptides form a multimeric structure that is responsible for its DNA-binding activity. Recombination-associated motifs and translin-binding sites are present at recombination hotspots and may serve as indicators of breakpoints in genes which are fused by translocations. These binding activities may play a crucial role in chromosomal translocation in lymphoid neoplasms. This protein encoded by this gene, when complexed with translin-associated protein X, also forms a Mg ion-dependent endoribonuclease that promotes RNA-induced silencing complex (RISC) activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2012]

Uniprot Description

TSN: DNA-binding protein that specifically recognizes consensus sequences at the breakpoint junctions in chromosomal translocations, mostly involving immunoglobulin (Ig)/T-cell receptor gene segments. Seems to recognize single-stranded DNA ends generated by staggered breaks occurring at recombination hot spots. Belongs to the translin family.

Protein type: Nuclear receptor co-regulator; DNA-binding; EC 3.1.-.-

Chromosomal Location of Human Ortholog: 2q21.1

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: endoribonuclease activity; mRNA binding; protein binding; DNA binding; sequence-specific DNA binding; protein complex binding; single-stranded DNA binding

Biological Process: gene expression; DNA recombination