Western Blot (WB)
(TSN Antibody (Center) western blot analysis in HL-60,Jurkat,K562 cell line mouse spleen tissue lysates (35ug/lane).This demonstrates the TSN antibody detected the TSN protein (arrow).)
Rabbit anti-Human TSN Polyclonal Antibody | anti-TSN antibody
TSN, ID (TSN, Translin, Component 3 of promoter of RISC)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Western Blot (WB)
(TSN Antibody (Center) western blot analysis in HL-60,Jurkat,K562 cell line mouse spleen tissue lysates (35ug/lane).This demonstrates the TSN antibody detected the TSN protein (arrow).)
Western Blot (WB)
(TSN Antibody (Center) western blot analysis in HL-60,Jurkat,K562 cell line mouse spleen tissue lysates (35ug/lane).This demonstrates the TSN antibody detected the TSN protein (arrow).)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with TSN Antibody(Center) .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSN primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).TSN immunoreactivity is localized to Nucleus and Cytoplasm significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with TSN Antibody(Center) .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSN primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).TSN immunoreactivity is localized to Nucleus and Cytoplasm significantly.)
Immunohistochemistry (IHC)
(TSN Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TSN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(TSN Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TSN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
Uniprot Description
Function: DNA-binding protein that specifically recognizes consensus sequences at the breakpoint junctions in chromosomal translocations, mostly involving immunoglobulin (Ig)/T-cell receptor gene segments. Seems to recognize single-stranded DNA ends generated by staggered breaks occurring at recombination hot spots
By similarity.Exhibits both single-stranded and double-stranded endoribonuclease activity. May act as an activator of RNA-induced silencing complex (RISC) by facilitating endonucleolytic cleavage of the siRNA passenger strand
By similarity.
Subunit structure: Ring-shaped heterooctamer of six TSN and two TSNAX subunits, DNA/RNA binding occurs inside the ring
By similarity.
Subcellular location: Cytoplasm
By similarity. Nucleus
By similarity.
Sequence similarities: Belongs to the translin family.
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Product Notes
The TSN tsn (Catalog #AAA641850) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TSN, ID (TSN, Translin, Component 3 of promoter of RISC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TSN can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50. Researchers should empirically determine the suitability of the TSN tsn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TSN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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