Rabbit anti-Human TSHB Polyclonal Antibody | anti-TSHB antibody

TSHB, ID (Thyrotropin Subunit beta, Thyrotropin beta Chain, Thyroid-stimulating Hormone Subunit beta, TSH-beta, TSH-B) (PE)

Cat. Num. AAA6502612

• Gene Names: TSHB; TSH-B; TSH-BETA
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: TSHB; Polyclonal Antibody; ID (Thyrotropin Subunit beta; Thyrotropin beta Chain; Thyroid-stimulating Hormone Subunit beta; TSH-beta; TSH-B) (PE); anti-TSHB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TSHB.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-TSHB antibody: Western Blot (WB), FLISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 58-86 in the central region of human TSHB, UniProt Accession #P01222. Species sequence homology: mouse
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS627402 in MDA-MB231 cell line lysates (35ug/lane). TSHB (arrow) was detected usingMBS627402.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TT5400-41C, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Flow Cytometry (FC/FACS)

(Fow cytometric analysis of MDA-MB231 cells (bottom histogram) using MBS627402 compared to a negative control cell (top histogram0. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of MBS627402 with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-TSHB antibody

Antibodies; Hormones; Steroids

NCBI and Uniprot Product Information

• NCBI GI #: 115430099
• NCBI GeneID: 7252
• NCBI Accession #: NP_000540.2
• NCBI GenBank Nucleotide #: NM_000549.4
• UniProt Accession #: P01222; Q16163; B1AKP0
• Molecular Weight: 10,624 Da
• NCBI Official Full Name: thyrotropin subunit beta isoform 1
• NCBI Official Synonym Full Names: thyroid stimulating hormone, beta
• NCBI Official Symbol: TSHB
• NCBI Official Synonym Symbols: TSH-B; TSH-BETA
• NCBI Protein Information: thyrotropin subunit beta; thyrotropin beta chain
• UniProt Protein Name: Thyrotropin subunit beta
• Protein Family: Thyrotropin
• UniProt Gene Name: TSHB
• UniProt Synonym Gene Names: TSH-B; TSH-beta
• UniProt Entry Name: TSHB_HUMAN

NCBI Description

The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. Thyroid stimulating hormone functions in the control of thyroid structure and metabolism. The protein encoded by this gene is the beta subunit of thyroid stimulating hormone. Mutations in this gene are associated with congenital central and secondary hypothyroidism and Hashimoto's thyroiditis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]

Uniprot Description

TSHB: Indispensable for the control of thyroid structure and metabolism. Belongs to the glycoprotein hormones subunit beta family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p13

Cellular Component: extracellular region

Molecular Function: hormone activity

Biological Process: G-protein coupled receptor protein signaling pathway; anatomical structure morphogenesis; cellular protein metabolic process; cell-cell signaling; response to estrogen stimulus; peptide hormone processing; response to calcium ion; response to vitamin A

Disease: Hypothyroidism, Congenital, Nongoitrous, 4