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Western Blot (WB) (Western Blot (WB) analysis of TSG-6 (V37) polyclonal antibody in extracts from MCF7 cells.)

Rabbit anti-Human, Mouse TSG-6 Polyclonal Antibody | anti-TSG-6 antibody

TSG-6 (V37) Polyclonal Antibody

Gene Names
TNFAIP6; TSG6; TSG-6
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
TSG-6; Polyclonal Antibody; TSG-6 (V37) Polyclonal Antibody; TNFAIP6; TNF-stimulated gene 6 protein; TSG6; anti-TSG-6 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
TSG-6 (V37) polyclonal antibody detects endogenous levels of TSG-6 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
277
Applicable Applications for anti-TSG-6 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Preparation and Storage
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of TSG-6 (V37) polyclonal antibody in extracts from MCF7 cells.)

Western Blot (WB) (Western Blot (WB) analysis of TSG-6 (V37) polyclonal antibody in extracts from MCF7 cells.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of TSG-6 (V37) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC) (Immunohistochemistry (IHC) analyzes of TSG-6 (V37) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)
Related Product Information for anti-TSG-6 antibody
TSG6 is a secretory protein that contains a hyaluronan-binding domain, and thus is a member of the hyaluronan-binding protein family. The hyaluronan-binding domain is involved in extracellular matrix stability and cell migration. This protein forms a stable complex with inter-alpha-inhibitor (I alpha I), and thus enhances the serine protease inhibitory activity of I alpha I, which is important in the protease network associated with inflammation. The expression of this gene can be induced by tumor necrosis factor alpha and interleukin-1. The expression can also be induced by mechanical stimuli in vascular smooth muscle cells, and is correlated with proteoglycan synthesis and aggregation.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~32kDa
NCBI Official Full Name
tumor necrosis factor-inducible gene 6 protein
NCBI Official Synonym Full Names
TNF alpha induced protein 6
NCBI Official Symbol
TNFAIP6
NCBI Official Synonym Symbols
TSG6; TSG-6
NCBI Protein Information
tumor necrosis factor-inducible gene 6 protein
UniProt Protein Name
Tumor necrosis factor-inducible gene 6 protein
UniProt Gene Name
TNFAIP6
UniProt Synonym Gene Names
TSG6; TSG-6; TNF alpha-induced protein 6

NCBI Description

The protein encoded by this gene is a secretory protein that contains a hyaluronan-binding domain, and thus is a member of the hyaluronan-binding protein family. The hyaluronan-binding domain is known to be involved in extracellular matrix stability and cell migration. This protein has been shown to form a stable complex with inter-alpha-inhibitor (I alpha I), and thus enhance the serine protease inhibitory activity of I alpha I, which is important in the protease network associated with inflammation. This gene can be induced by proinflammatory cytokines such as tumor necrosis factor alpha and interleukin-1. Enhanced levels of this protein are found in the synovial fluid of patients with osteoarthritis and rheumatoid arthritis.[provided by RefSeq, Dec 2010]

Uniprot Description

TNFAIP6: Possibly involved in cell-cell and cell-matrix interactions during inflammation and tumorigenesis.

Chromosomal Location of Human Ortholog: 2q23.3

Molecular Function: protein binding

Biological Process: cell-cell signaling; inflammatory response; negative regulation of inflammatory response; signal transduction

Research Articles on TSG-6

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Product Notes

The TSG-6 tnfaip6 (Catalog #AAA3002899) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TSG-6 (V37) Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TSG-6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:1000 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the TSG-6 tnfaip6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TSG-6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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