Rabbit anti-Human TSEN2 Polyclonal Antibody | anti-TSEN2 antibody

TSEN2, NT (TSEN2, SEN2, tRNA-splicing endonuclease subunit Sen2, tRNA-intron endonuclease Sen2) (FITC)

Cat. Num. AAA6359422

• Gene Names: TSEN2; SEN2; PCH2B; SEN2L
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TSEN2; Polyclonal Antibody; NT (TSEN2; SEN2; tRNA-splicing endonuclease subunit Sen2; tRNA-intron endonuclease Sen2) (FITC); tRNA-intron endonuclease Sen2; anti-TSEN2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-TSEN2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TSEN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-43 amino acids from the N-terminal region of human TSEN2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TSEN2 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the TSEN2 antibody detected the TSEN2 protein (arrow).)

Related Product Information for anti-TSEN2 antibody

This gene encodes one of the subunits of the tRNA splicing endonuclease. This endonuclease catalyzes the first step in RNA splicing which is the removal of introns. Mutations in this gene have been associated with pontocerebellar hypoplasia type 2. A pseudogene has been identified on chromosome 4. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-TSEN2 antibody

Antibodies; Enzymes; Endonuclease

References

Weitzer, S., et al. Nature 447(7141):222-226(2007). Lamesch, P., et al. Genomics 89(3):307-315(2007). Ballif, B.A., et al. Mol. Cell Proteomics 3(11):1093-1101(2004). Paushkin, S.V., et al. Cell 117(3):311-321(2004). Nakayama, M., et al. Genome Res. 12(11):1773-1784(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 223972632
• NCBI GeneID: 80746
• NCBI Accession #: NP_001138864.1
• NCBI GenBank Nucleotide #: NM_001145392.1
• UniProt Accession #: Q8NCE0; Q8WTW7; Q9BPU7; B7Z6K1; C9IZI7; G5E9Q3
• Molecular Weight: 53,247 Da
• NCBI Official Full Name: tRNA-splicing endonuclease subunit Sen2 isoform 1
• NCBI Official Synonym Full Names: TSEN2 tRNA splicing endonuclease subunit
• NCBI Official Symbol: TSEN2
• NCBI Official Synonym Symbols: SEN2; PCH2B; SEN2L
• NCBI Protein Information: tRNA-splicing endonuclease subunit Sen2; hsSen2; tRNA-intron endonuclease Sen2
• UniProt Protein Name: tRNA-splicing endonuclease subunit Sen2
• UniProt Gene Name: TSEN2
• UniProt Synonym Gene Names: SEN2; HsSen2
• UniProt Entry Name: SEN2_HUMAN

Uniprot Description

Function: Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Isoform 1 probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. Isoform 2 is responsible for processing a yet unknown RNA substrate. The complex containing isoform 2 is not able to cleave pre-tRNAs properly, although it retains endonucleolytic activity. Ref.1

Catalytic activity: Endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxy and 2',3'-cyclic phosphate termini, and specifically removing the intron.

Subunit structure: tRNA splicing endonuclease is a heterotetramer composed of isoform 1 of SEN2, SEN15, SEN34/LENG5 and SEN54. tRNA splicing endonuclease complex also contains proteins of the pre-mRNA 3'-end processing machinery such as CLP1, CPSF1, CPSF4 and CSTF2. Isoform 2 belongs to a different complex that contains SEN54 but low level of SEN15 and SEN34/LENG5.

Subcellular location: Nucleus. Nucleus › nucleolus. Note: May be transiently localized in the nucleolus. Ref.1

Tissue specificity: Isoform 1 and isoform 2 are widely expressed at very low level. Ref.1

Involvement in disease: Pontocerebellar hypoplasia 2B (PCH2B) [MIM:612389]: A disorder characterized by an abnormally small cerebellum and brainstem, and progressive microcephaly from birth combined with extrapyramidal dyskinesia. Severe chorea occurs and epilepsy is frequent. There are no signs of spinal cord anterior horn cells degeneration.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.7

Sequence similarities: Belongs to the tRNA-intron endonuclease family.