Rabbit anti-Mouse TSC2 Polyclonal Antibody | anti-TSC2 antibody

TSC2, Phosphorylated (S1412) (Tsc2, Tuberin, Tuberous sclerosis 2 protein homolog) (PE)

Cat. Num. AAA6359363

• Gene Names: Tsc2; Tcs2; Nafld
• Reactivity: Mouse
• Applications: Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TSC2; Polyclonal Antibody; Phosphorylated (S1412) (Tsc2; Tuberin; Tuberous sclerosis 2 protein homolog) (PE); Tsc2; Tuberous sclerosis 2 protein homolog; anti-TSC2 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-TSC2 antibody: FLISA, Dot Blot (DB)
• Application Notes: FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: mouse TSC2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1412 of mouse TSC2.
• Conjugate: PE
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of mouse TSC2 Antibody (Phospho S1412) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

Related Product Information for anti-TSC2 antibody

In complex with TSC1, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling (By similarity). Specifically stimulates the intrinsic GTPase activity of the Ras-related protein RAP1A and RAB5. Suggesting a possible mechanism for its role in regulating cellular growth (By similarity).

Product Categories/Family for anti-TSC2 antibody

Antibodies; Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 9297076
• NCBI GeneID: 22084
• UniProt Accession #: Q61037; P97723; P97724; P97725; P97727; Q61007; Q61008; Q9WUF6
• Molecular Weight: 189,855 Da
• NCBI Official Full Name: Tuberin
• NCBI Official Synonym Full Names: tuberous sclerosis 2
• NCBI Official Symbol: Tsc2
• NCBI Official Synonym Symbols: Tcs2; Nafld
• NCBI Protein Information: tuberin
• UniProt Protein Name: Tuberin
• Protein Family: Tuberin
• UniProt Gene Name: Tsc2

Uniprot Description

In complex with TSC1, this tumor suppressor inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. May also play a role in microtubule-mediated protein transport. Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5.