Rabbit anti-Human TSC1 Polyclonal Antibody | anti-TSC1 antibody

TSC1, Phosphorylated (S598) (TSC1, KIAA0243, TSC, Hamartin, Tuberous sclerosis 1 protein) (FITC)

Cat. Num. AAA6359092

• Gene Names: TSC1; LAM; TSC
• Reactivity: Human
• Applications: Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TSC1; Polyclonal Antibody; Phosphorylated (S598) (TSC1; KIAA0243; TSC; Hamartin; Tuberous sclerosis 1 protein) (FITC); Tuberous sclerosis 1 protein; anti-TSC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-TSC1 antibody: FLISA, Dot Blot (DB)
• Application Notes: FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TSC1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S598 of human TSC1.
• Conjugate: FITC
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of TSC1 Antibody (Phospho S598) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

Related Product Information for anti-TSC1 antibody

This gene encodes a growth inhibitory protein thought to play a role in the stabilization of tuberin. Mutations in this gene have been associated with tuberous sclerosis. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-TSC1 antibody

Antibodies; Disease Markers

References

Hoogeveen-Westerveld, M., et al. Biochim. Biophys. Acta 1802(9):774-781(2010). Mehta, M.S., et al. Breast Cancer Res. Treat. (2010). In press: Mieulet, V., et al. Trends Mol Med 16(7):329-335(2010). Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010). Guo, L., et al. Acta Biochim. Biophys. Sin. (Shanghai) 42(4):266-273(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4507693
• NCBI GeneID: 7248
• NCBI Accession #: NP_000359.1
• NCBI GenBank Nucleotide #: NM_000368.4
• UniProt Accession #: Q92574; Q5VVN5; B7Z897
• Molecular Weight: 1164
• NCBI Official Full Name: hamartin isoform 1
• NCBI Official Synonym Full Names: tuberous sclerosis 1
• NCBI Official Symbol: TSC1
• NCBI Official Synonym Symbols: LAM; TSC
• NCBI Protein Information: hamartin; tumor suppressor; tuberous sclerosis 1 protein
• UniProt Protein Name: Hamartin
• Protein Family: Tuberous sclerosis 1 protein
• UniProt Gene Name: TSC1
• UniProt Synonym Gene Names: KIAA0243; TSC
• UniProt Entry Name: TSC1_HUMAN

Uniprot Description

TSC1: tuberous sclerosis protein 1 (TSC1). May have tumor suppressor activity. Together with tuberin (TSC2), inhibits mammalian target of rapamycin (mTOR)-mediated signaling to eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) and ribosomal protein S6 kinase. Regulated by the phosphatidylinositol 3-kinase/Akt pathway and phosphorylation of tuberin. Phosphorylated by cyclin-dependent kinase 1/cyclin B during the cell cycle.

Protein type: Tumor suppressor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: TSC1-TSC2 complex; growth cone; protein complex; membrane; intracellular membrane-bound organelle; lamellipodium; cytoplasm; plasma membrane; actin filament; cell cortex; cytosol

Molecular Function: protein binding; chaperone binding; protein N-terminus binding; GTPase activating protein binding; GTPase regulator activity

Biological Process: myelination; regulation of cell cycle; protein heterooligomerization; regulation of cell-matrix adhesion; cell-matrix adhesion; negative regulation of insulin receptor signaling pathway; negative regulation of cell size; regulation of phosphoprotein phosphatase activity; cardiac muscle cell differentiation; response to insulin stimulus; regulation of translation; negative regulation of cell proliferation; positive regulation of focal adhesion formation; cell projection organization and biogenesis; synapse organization and biogenesis; cell cycle arrest; kidney development; potassium ion transport; negative regulation of TOR signaling pathway; protein stabilization; hippocampus development; rRNA export from nucleus; negative regulation of translation; glucose import; regulation of stress fiber formation; neural tube closure; insulin receptor signaling pathway; cerebral cortex development; regulation of protein kinase activity

Disease: Lymphangioleiomyomatosis; Focal Cortical Dysplasia Of Taylor; Tuberous Sclerosis 1