Rabbit anti-Human TRRAP Polyclonal Antibody | anti-TRRAP antibody

TRRAP Antibody (C-term)

Cat. Num. AAA9215621

• Reactivity: Human
• Applications: Western Blot
• Synonyms: TRRAP; Polyclonal Antibody; TRRAP Antibody (C-term); Purified Rabbit Polyclonal Antibody (Pab); Transformation/transcription domain-associated protein; 350/400 kDa PCAF-associated factor; PAF350/400; STAF40; Tra1 homolog; PAF400; anti-TRRAP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This TRRAP antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 3015-3049 amino acids from the C-terminal region of human TRRAP.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 3859

• Applicable Applications for anti-TRRAP antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:2000; WB ~~ 1:2000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-TRRAP Antibody (C-term)at 1:2000 dilution + K562 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 438 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(All lanes : Anti-TRRAP Antibody (C-term) at 1:2000 dilutionLane 1: K562 whole cell lysatesLane 2: Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 438 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-TRRAP antibody

Adapter protein, which is found in various multiprotein chromatin complexes with histone acetyltransferase activity (HAT), which gives a specific tag for epigenetic transcription activation. Component of the NuA4 histone acetyltransferase complex which is responsible for acetylation of nucleosomal histones H4 and H2A. Plays a central role in MYC transcription activation, and also participates in cell transformation by MYC. Required for p53/TP53-, E2F1- and E2F4-mediated transcription activation. Also involved in transcription activation mediated by the adenovirus E1A, a viral oncoprotein that deregulates transcription of key genes. Probably acts by linking transcription factors such as E1A, MYC or E2F1 to HAT complexes such as STAGA thereby allowing transcription activation. Probably not required in the steps following histone acetylation in processes of transcription activation. May be required for the mitotic checkpoint and normal cell cycle progression. Component of a SWR1- like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome.

Product Categories/Family for anti-TRRAP antibody

Crown Antibodies

References

McMahon S.B.,et al.Cell 94:363-374(1998). Vassilev A.,et al.Mol. Cell 2:869-875(1998). Hillier L.W.,et al.Nature 424:157-164(2003). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. Scherer S.W.,et al.Science 300:767-772(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 347360922
• NCBI GeneID: 8295
• NCBI Accession #: NP_001231509.1
• NCBI GenBank Nucleotide #: NM_001244580.1
• UniProt Accession #: Q9Y4A5; O75218; Q9Y631; Q9Y6H4; A4D265
• Molecular Weight: 437600
• NCBI Official Full Name: transformation/transcription domain-associated protein isoform 1
• UniProt Protein Name: Transformation/transcription domain-associated protein
• Protein Family: Transformation/transcription domain-associated protein
• UniProt Gene Name: TRRAP
• UniProt Synonym Gene Names: PAF400; PAF350/400
• UniProt Entry Name: TRRAP_HUMAN

NCBI Description

TRRAP: Adapter protein, which is found in various multiprotein chromatin complexes with histone acetyltransferase activity (HAT), which gives a specific tag for epigenetic transcription activation. Component of the NuA4 histone acetyltransferase complex which is responsible for acetylation of nucleosomal histones H4 and H2A. Plays a central role in MYC transcription activation, and also participates in cell transformation by MYC. Required for p53/TP53-, E2F1- and E2F4-mediated transcription activation. Also involved in transcription activation mediated by the adenovirus E1A, a viral oncoprotein that deregulates transcription of key genes. Probably acts by linking transcription factors such as E1A, MYC or E2F1 to HAT complexes such as STAGA thereby allowing transcription activation. Probably not required in the steps following histone acetylation in processes of transcription activation. May be required for the mitotic checkpoint and normal cell cycle progression. Interacts with MYC, E2F1 and E2F4 transcription factors. Interacts directly with p53/TP53. Interacts with GCN5L2. Component of various HAT complexes. Component of the PCAF complex, at least composed of TADA2L/ADA2, SUPT3H, TADA3L/ADA3, TAF5L/PAF65-beta, TAF6L/PAF65-alpha, TAF10/TAFII30, TAF12/TAFII20, TAF9/TAFII31 and TRRAP. Component of the TFTC-HAT complex, at least composed of TAF5L, TAF6L, TADA3L, SUPT3H/SPT3, TAF2/TAFII150, TAF4/TAFII135, TAF5/TAFII100, GCN5L2/GCN5, TAF10 and TRRAP. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. Component of the STAGA complex, at least composed of SUPT3H, GCN5L2, SUPT7L, TAF5L, TAF6L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9. The STAGA core complex is associated with a subcomplex required for histone deubiquitination composed of ATXN7L3, ENY2 and USP22. Component of the BAF53 complex, at least composed of BAF53A, RUVBL1, SMARCA4/BRG1, and TRRAP, which preferentially acetylates histone H4 (and H2A) within nucleosomes. Interacts with NPAT. Interaction with TELO2 AND TTI1. Belongs to the PI3/PI4-kinase family. TRA1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Protein kinase, atypical; Kinase, protein; Nuclear receptor co-regulator; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; PIKK family; TRRAP subfamily

Chromosomal Location of Human Ortholog: 7q21.2-q22.1

Cellular Component: nucleoplasm; Golgi apparatus; PCAF complex; NuA4 histone acetyltransferase complex; nucleus

Molecular Function: protein binding; transcription coactivator activity; transcription cofactor activity; kinase activity; phosphotransferase activity, alcohol group as acceptor

Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of transcription, DNA-dependent; mitotic cell cycle checkpoint; histone acetylation; DNA repair; phosphorylation; histone deubiquitination

Uniprot Description

TRRAP: Adapter protein, which is found in various multiprotein chromatin complexes with histone acetyltransferase activity (HAT), which gives a specific tag for epigenetic transcription activation. Component of the NuA4 histone acetyltransferase complex which is responsible for acetylation of nucleosomal histones H4 and H2A. Plays a central role in MYC transcription activation, and also participates in cell transformation by MYC. Required for p53/TP53-, E2F1- and E2F4-mediated transcription activation. Also involved in transcription activation mediated by the adenovirus E1A, a viral oncoprotein that deregulates transcription of key genes. Probably acts by linking transcription factors such as E1A, MYC or E2F1 to HAT complexes such as STAGA thereby allowing transcription activation. Probably not required in the steps following histone acetylation in processes of transcription activation. May be required for the mitotic checkpoint and normal cell cycle progression. Interacts with MYC, E2F1 and E2F4 transcription factors. Interacts directly with p53/TP53. Interacts with GCN5L2. Component of various HAT complexes. Component of the PCAF complex, at least composed of TADA2L/ADA2, SUPT3H, TADA3L/ADA3, TAF5L/PAF65-beta, TAF6L/PAF65-alpha, TAF10/TAFII30, TAF12/TAFII20, TAF9/TAFII31 and TRRAP. Component of the TFTC-HAT complex, at least composed of TAF5L, TAF6L, TADA3L, SUPT3H/SPT3, TAF2/TAFII150, TAF4/TAFII135, TAF5/TAFII100, GCN5L2/GCN5, TAF10 and TRRAP. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. Component of the STAGA complex, at least composed of SUPT3H, GCN5L2, SUPT7L, TAF5L, TAF6L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9. The STAGA core complex is associated with a subcomplex required for histone deubiquitination composed of ATXN7L3, ENY2 and USP22. Component of the BAF53 complex, at least composed of BAF53A, RUVBL1, SMARCA4/BRG1, and TRRAP, which preferentially acetylates histone H4 (and H2A) within nucleosomes. Interacts with NPAT. Interaction with TELO2 AND TTI1. Belongs to the PI3/PI4-kinase family. TRA1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, atypical; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Transcription, coactivator/corepressor; Nuclear receptor co-regulator; ATYPICAL group; PIKK family; TRRAP subfamily

Chromosomal Location of Human Ortholog: 7q21.2-q22.1

Cellular Component: Golgi apparatus; NuA4 histone acetyltransferase complex; nucleoplasm; nucleus; PCAF complex

Molecular Function: kinase activity; phosphotransferase activity, alcohol group as acceptor; protein binding; transcription coactivator activity; transcription cofactor activity

Biological Process: DNA repair; establishment and/or maintenance of chromatin architecture; histone acetylation; histone deubiquitination; mitotic cell cycle checkpoint; phosphorylation; regulation of transcription, DNA-dependent; transcription, DNA-dependent