Rabbit TRPV6 Polyclonal Antibody | anti-TRPV6 antibody

TRPV6 Antibody

Cat. Num. AAA9611535

• Reactivity: Human, Mouse, Rat; Predicted: Horse, Rabbit, Dog
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: TRPV6; Polyclonal Antibody; TRPV6 Antibody; anti-TRPV6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Horse, Rabbit, Dog
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TRPV6 Antibody detects endogenous levels of TRPV6.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 725

• Applicable Applications for anti-TRPV6 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human TRPV6.
• Tissue Specificity: Expressed at high levels in the gastrointestinal tract, including esophagus, stomach, duodenum, jejunum, ileum and colon, and in pancreas, placenta, prostate and salivary gland. Expressed at moderate levels in liver, kidney and testis. Expressed in trophoblasts of placenta villus trees (at protein level)(PubMed:23612980). Expressed in locally advanced prostate cancer, metastatic and androgen-insensitive prostatic lesions but not detected in healthy prostate tissue and benign prostatic hyperplasia.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Plasma Membrane
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Rat brain, using TRPV6 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 70 kDa.)

Western Blot (WB)

(Western blot analysis of extracts from PC-3 cells, using TRPV6 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-TRPV6 antibody

Description: Calcium selective cation channel that mediates Ca2+ uptake in various tissues, including the intestine (PubMed:11097838, PubMed:11278579, PubMed:11248124 PubMed:15184369, PubMed:23612980, PubMed:29258289). Important for normal Ca2+ ion homeostasis in the body, including bone and skin (By similarity). The channel is activated by low internal calcium level, probably including intracellular calcium store depletion, and the current exhibits an inward rectification (PubMed:15184369). Inactivation includes both a rapid Ca2+-dependent and a slower Ca2+-calmodulin-dependent mechanism; the latter may be regulated by phosphorylation. In vitro, is slowly inhibited by Mg2+ in a voltage-independent manner. Heteromeric assembly with TRPV5 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating.

Post Translational Modifications: Glycosylated. Phosphorylation at Tyr-201 by SRC leads to an increased calcium influx through the channel. Probably dephosphorylated at this site by PTPN1 (By similarity). Phosphorylation by PRKCA at the calmodulin binding site delays channel inactivation (PubMed:11248124).

Subunit Structure: Homotetramer (PubMed:29258289). Probably forms also heterotetramers with TRPV5. Interacts with TRPV5. Interacts with S100A10 and probably with the ANAX2-S100A10 heterotetramer. The interaction with S100A10 is required for the trafficking to the plasma membrane. Interacts with BSPRY (By similarity). Interacts with TCAF1 and TCAF2 isoform 2 (PubMed:25559186). Interacts with calmodulin (PubMed:11248124).

Similarity: Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV6 sub-subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 62901469
• UniProt Accession #: Q9H1D0
• Molecular Weight: Observed: 83 kDa; Predicted: 88 kDa
• NCBI Official Full Name: Transient receptor potential cation channel subfamily V member 6
• UniProt Protein Name: Transient receptor potential cation channel subfamily V member 6
• Protein Family: Transient receptor potential cation channel subfamily
• UniProt Gene Name: TRPV6
• UniProt Synonym Gene Names: ECAC2; TrpV6; CaT-L; CaT1; ECaC2
• UniProt Entry Name: TRPV6_HUMAN

Uniprot Description

TRPV6: Calcium selective cation channel probably involved in Ca(2+) uptake in various tissues, including Ca(2+) reabsorption in intestine. The channel is activated by low internal calcium level, probably including intracellular calcium store depletion, and the current exhibits an inward rectification. Inactivation includes both, a rapid Ca(2+)-dependent and a slower Ca(2+)-calmodulin- dependent mechanism, the latter may be regulated by phosphorylation. In vitro, is slowly inhibited by Mg(2+) in a voltage-independent manner. Heteromeric assembly with TRPV5 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating. Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV6 sub-subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Channel, calcium

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: calmodulin binding; protein binding; calcium channel activity

Biological Process: calcium ion transport; transmembrane transport; regulation of calcium ion-dependent exocytosis