Rabbit TRPV4 Polyclonal Antibody | anti-TRPV4 antibody

TRPV4 Antibody - N-terminal region

Cat. Num. AAA3202531

• Gene Names: TRPV4; SMAL; VRL2; BCYM3; CMT2C; SPSMA; TRP12; VROAC; HMSN2C; OTRPC4; SSQTL1
• Reactivity: Tested Reactivity: Human; Predicted Reactivity: Cow, Dog, Human, Mouse, Pig, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified
• Synonyms: TRPV4; Polyclonal Antibody; TRPV4 Antibody - N-terminal region; anti-TRPV4 antibody

• Host: Rabbit
• Reactivity: Tested Reactivity: Human; Predicted Reactivity: Cow, Dog, Human, Mouse, Pig, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity Purified
• Form/Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: Batch dependent within range: 100 ul at 0.5 - 1 mg/m1 (varies by lot)

• Applicable Applications for anti-TRPV4 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Protein Size (# AA): 552 amino acids
• Blocking Peptide: For anti-TRPV4 (MBS3202531) antibody is MBS3227508
• Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of human TRPV4
• Replacement Item: This antibody may replace item sc-16485 from Santa Cruz Biotechnology.
• Predicted Homology: Cow: 85%; Dog: 92%; Human: 100%; Mouse: 86%; Pig: 92%; Rat: 85%
• Preparation and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(Rabbit Anti-TRPV4 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Adult heartObserved Staining: CytoplasmicPrimary Antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy2/3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 secProtocol located in Reviews and Data.)

Western Blot (WB)

(Host: RabbitTarget Name: TRPV4Sample Type: HT1080 Whole cell lysatesAntibody Dilution: 1.0ug/ml)

Western Blot (WB)

(WB Suggested Anti-TRPV4 antibody Titration: 1 ug/mLSample Type: Human heart)

Related Product Information for anti-TRPV4 antibody

This gene encodes a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. The encoded protein is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure. Mutations in this gene are the cause of spondylometaphyseal and metatropic dysplasia and hereditary motor and sensory neuropathy type IIC. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-TRPV4 antibody

Polyclonal; Various

NCBI and Uniprot Product Information

• NCBI GI #: 294459965
• NCBI GeneID: 59341
• NCBI Accession #: NP_001170899.1
• NCBI GenBank Nucleotide #: NM_001177428.1
• UniProt Accession #: Q9HBA0
• Molecular Weight: 60kDa
• NCBI Official Full Name: transient receptor potential cation channel subfamily V member 4 isoform e
• NCBI Official Synonym Full Names: transient receptor potential cation channel subfamily V member 4
• NCBI Official Symbol: TRPV4
• NCBI Official Synonym Symbols: SMAL; VRL2; BCYM3; CMT2C; SPSMA; TRP12; VROAC; HMSN2C; OTRPC4; SSQTL1
• NCBI Protein Information: transient receptor potential cation channel subfamily V member 4
• UniProt Protein Name: Transient receptor potential cation channel subfamily V member 4
• Protein Family: Transient receptor potential cation channel subfamily
• UniProt Gene Name: TRPV4
• UniProt Synonym Gene Names: VRL2; VROAC; TrpV4; OTRPC4; TRP12; VRL-2; VR-OAC
• UniProt Entry Name: TRPV4_HUMAN

NCBI Description

This gene encodes a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. The encoded protein is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure. Mutations in this gene are the cause of spondylometaphyseal and metatropic dysplasia and hereditary motor and sensory neuropathy type IIC. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]

Uniprot Description

TRPV4: a non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8. Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV4 sub-subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, cation; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q24.1

Cellular Component: cortical actin cytoskeleton; cell surface; focal adhesion; growth cone; adherens junction; lamellipodium; cytoplasmic microtubule; plasma membrane; integral to membrane; cytoplasmic vesicle; filopodium; cilium

Molecular Function: calcium channel activity; microtubule binding; beta-tubulin binding; actin binding; protein kinase binding; calmodulin binding; actin filament binding; protein binding; protein kinase C binding; osmosensor activity; SH2 domain binding; cation channel activity; alpha-tubulin binding; ATP binding

Biological Process: intercellular junction assembly; actin filament organization; regulation of response to osmotic stress; osmosensory signaling pathway; cellular calcium ion homeostasis; vasopressin secretion; positive regulation of microtubule depolymerization; elevation of cytosolic calcium ion concentration; hyperosmotic salinity response; response to mechanical stimulus; calcium ion transport; actin cytoskeleton reorganization; cell volume homeostasis; cortical microtubule organization and biogenesis; transmembrane transport; microtubule polymerization

Disease: Brachyolmia Type 3; Scapuloperoneal Spinal Muscular Atrophy; Hereditary Motor And Sensory Neuropathy, Type Iic; Sodium Serum Level Quantitative Trait Locus 1; Spondyloepiphyseal Dysplasia, Maroteaux Type; Parastremmatic Dwarfism; Spinal Muscular Atrophy, Distal, Congenital Nonprogressive; Digital Arthropathy-brachydactyly, Familial; Metatropic Dysplasia; Spondylometaphyseal Dysplasia, Kozlowski Type