Rabbit anti-Human TRPM8 Polyclonal Antibody | anti-TRPM8 antibody

TRPM8, ID (TRPM8, LTRPC6, TRPP8, Transient receptor potential cation channel subfamily M member 8, Long transient receptor potential channel 6, Transient receptor potential p8) (AP)

Cat. Num. AAA6358869

• Gene Names: TRPM8; TRPP8; LTRPC6
• Reactivity: Human
• Applications: Immunofluorescence, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: TRPM8; Polyclonal Antibody; ID (TRPM8; LTRPC6; TRPP8; Transient receptor potential cation channel subfamily M member 8; Long transient receptor potential channel 6; Transient receptor potential p8) (AP); Transient receptor potential p8; anti-TRPM8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-TRPM8 antibody: Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: TRPM8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-300 amino acids from the Central region of human TRPM8.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TRPM8 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TRPM8 antibody detected the TRPM8 protein (arrow).)

Immunohistochemistry (IHC)

(TRPM8 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRPM8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of TRPM8 Antibody (Center) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-TRPM8 antibody

Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.

Product Categories/Family for anti-TRPM8 antibody

Antibodies; Receptors

References

Yee, N.S., et al. Cancer Lett. 297(1):49-55(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Kuhn, F.J., et al. J. Biol. Chem. 285(35):26806-26814(2010). Gkika, D., et al. Oncogene 29(32):4611-4616(2010). Van Haute, C., et al. ScientificWorldJournal 10, 1597-1611 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 109689695
• NCBI GeneID: 79054
• NCBI Accession #: NP_076985.4
• NCBI GenBank Nucleotide #: NM_024080.4
• UniProt Accession #: Q7Z2W7; Q3YFM7; Q6QNH9; Q8TAC3; Q8TDX8; Q9BVK1; A0AVG2
• Molecular Weight: 79,096 Da
• NCBI Official Full Name: transient receptor potential cation channel subfamily M member 8
• NCBI Official Synonym Full Names: transient receptor potential cation channel subfamily M member 8
• NCBI Official Symbol: TRPM8
• NCBI Official Synonym Symbols: TRPP8; LTRPC6
• NCBI Protein Information: transient receptor potential cation channel subfamily M member 8
• UniProt Protein Name: Transient receptor potential cation channel subfamily M member 8
• Protein Family: TRPM8 channel-associated factor
• UniProt Gene Name: TRPM8
• UniProt Synonym Gene Names: LTRPC6; TRPP8; LTrpC-6; LTrpC6; Trp-p8
• UniProt Entry Name: TRPM8_HUMAN

Uniprot Description

TRPM8: Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Channel, cation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: endoplasmic reticulum membrane; external side of plasma membrane; integral to membrane; lipid raft; plasma membrane

Molecular Function: calcium channel activity; protein binding; protein homodimerization activity

Biological Process: cellular calcium ion homeostasis; detection of temperature stimulus; protein homotetramerization; response to cold; thermoception