Rabbit anti-Human TRPM6 Polyclonal Antibody | anti-TRPM6 antibody

TRPM6, ID (Transient Receptor Potential Cation Channel Subfamily M Member 6, Channel-kinase 2, Melastatin-related TRP Cation Channel 6, CHAK2)

Cat. Num. AAA628253

• Gene Names: TRPM6; HSH; HMGX; HOMG; CHAK2; HOMG1
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: TRPM6; Polyclonal Antibody; ID (Transient Receptor Potential Cation Channel Subfamily M Member 6; Channel-kinase 2; Melastatin-related TRP Cation Channel 6; CHAK2); Anti -TRPM6; anti-TRPM6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CHAK2.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-TRPM6 antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the center region of human TRPM6 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CHAK2 Antibody (H332) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the CHAK2 antibody detected the CHAK2 protein (arrow).)

Immunohistochemistry (IHC)

(TRPM6(CHAK2) Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRPM6 Antibody (CHAK2) (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-TRPM6 antibody

CHAK2 is a member of the ion channel proteins. It regulates the calcium concentration in cells, which is imperative for such functions as muscle contractions and neuronal firing. It is also upregulated in certain forms of cancer and could play a role in diabetes.

Product Categories/Family for anti-TRPM6 antibody

Antibodies; Abs to Ion Channel

NCBI and Uniprot Product Information

• NCBI GI #: 293597572
• NCBI GeneID: 140803
• NCBI Accession #: NP_001170781.1
• NCBI GenBank Nucleotide #: NM_001177310.1
• UniProt Accession #: Q5VYG5; Q96LV9; Q9BX84; Q9H637; Q9NXR9
• Molecular Weight: 131,263 Da
• NCBI Official Full Name: transient receptor potential cation channel subfamily M member 6 isoform b
• NCBI Official Synonym Full Names: transient receptor potential cation channel, subfamily M, member 6
• NCBI Official Symbol: TRPM6
• NCBI Official Synonym Symbols: HSH; HMGX; HOMG; CHAK2; HOMG1
• NCBI Protein Information: transient receptor potential cation channel subfamily M member 6; channel kinase 2; OTTHUMP00000021487; melastatin-related TRP cation channel 6
• UniProt Protein Name: Transient receptor potential cation channel, subfamily M, member 6
• Protein Family: Transient receptor potential cation channel subfamily
• UniProt Gene Name: TRPM6
• UniProt Entry Name: Q5VYG5_HUMAN

NCBI Description

This gene is predominantly expressed in the kidney and colon, and encodes a protein containing an ion channel domain and a protein kinase domain. It is crucial for magnesium homeostasis, and plays an essential role in epithelial magnesium transport and in the active magnesium absorption in the gut and kidney. Mutations in this gene are associated with hypomagnesemia with secondary hypocalcemia. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq]

Uniprot Description

ChaK2: is an unusual bifunctional protein containing a transient receptor potential (TRP) ion channel fused to a protein kinase of the Atypical alpha group. Crucial for magnesium homeostasis. Has an important role in epithelial magnesium transport and in the active magnesium absorption in the gut and kidney. ChaK2 mutations cause hereditary hypomagnesemia and secondary hypocalcemia (HSH). The TRP voltage-independent calcium-permeable cation channels are divided into six subfa milies, two of which (TRPC and TRPM) have members that are widely expressed and activated by oxidative stress. Related to ChaK1, another Atypical alpha kinase fused to a TRP ion channel protein. Forms homodimers or heterodimers with ChaK1. Six alternatively spliced isoforms have been described, three of which lack the ion channel region.

Protein type: Protein kinase, atypical; Channel, cation; Membrane protein, integral; Kinase, protein; Membrane protein, multi-pass; EC 2.7.11.1; ATYPICAL group; Alpha family; ChaK subfamily

Chromosomal Location of Human Ortholog: 9q21.13

Cellular Component: brush border membrane; apical plasma membrane; integral to membrane; plasma membrane

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; calcium channel activity; ATP binding

Biological Process: response to toxin; transmembrane transport; protein amino acid phosphorylation

Disease: Hypomagnesemia 1, Intestinal