Rabbit anti-Human TRMT11 Polyclonal Antibody | anti-TRMT11 antibody

TRMT11, CT (TRMT11, C6orf75, tRNA (guanine(10)-N2)-methyltransferase homolog, tRNA guanosine-2'-O-methyltransferase TRM11 homolog) (Azide free) (HRP)

Cat. Num. AAA6358730

• Gene Names: TRMT11; TRM11; MDS024; C6orf75; TRMT11-1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TRMT11; Polyclonal Antibody; CT (TRMT11; C6orf75; tRNA (guanine(10)-N2)-methyltransferase homolog; tRNA guanosine-2'-O-methyltransferase TRM11 homolog) (Azide free) (HRP); tRNA guanosine-2'-O-methyltransferase TRM11 homolog; anti-TRMT11 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-TRMT11 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TRMT11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 310-340 amino acids from the C-terminal region of human TRMT11.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TRMT11 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the TRMT11 antibody detected the TRMT11 protein (arrow).)

Flow Cytometry (FC/FACS)

(TRMT11 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-TRMT11 antibody

Antibodies; Enzymes; Methyltransferase

References

Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005). Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005). Mungall, A.J., et al. Nature 425(6960):805-811(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 94420683
• NCBI GeneID: 60487
• NCBI Accession #: NP_001026882.2
• NCBI GenBank Nucleotide #: NM_001031712.2
• UniProt Accession #: Q7Z4G4; Q5JY11; Q6PGQ5; Q9HC13; E1P570
• Molecular Weight: 29,193 Da
• NCBI Official Full Name: tRNA (guanine(10)-N2)-methyltransferase homolog isoform a
• NCBI Official Synonym Full Names: tRNA methyltransferase 11 homolog
• NCBI Official Symbol: TRMT11
• NCBI Official Synonym Symbols: TRM11; MDS024; C6orf75; TRMT11-1
• NCBI Protein Information: tRNA (guanine(10)-N2)-methyltransferase homolog
• UniProt Protein Name: tRNA (guanine(10)-N2)-methyltransferase homolog
• Protein Family: tRNA
• UniProt Gene Name: TRMT11
• UniProt Synonym Gene Names: C6orf75

Uniprot Description

Catalytic subunit of an S-adenosyl-L-methionine-dependent tRNA methyltransferase complex that mediates the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs.