Western Blot (WB)
(TRIM59 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the TRIM59 antibody detected the TRIM59 protein (arrow).)
Rabbit anti-Human TRIM59 Polyclonal Antibody | anti-TRIM59 antibody
TRIM59, NT (TRIM59, RNF104, TRIM57, TSBF1, Tripartite motif-containing protein 59, RING finger protein 104, Tumor suppressor TSBF-1) (APC)
Gene Names
TRIM59; MRF1; TSBF1; RNF104; TRIM57
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
TRIM59; Polyclonal Antibody; NT (TRIM59; RNF104; TRIM57; TSBF1; Tripartite motif-containing protein 59; RING finger protein 104; Tumor suppressor TSBF-1) (APC); Tumor suppressor TSBF-1; anti-TRIM59 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-TRIM59 antibody
Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:10-50
FLISA: 1:1,000
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
TRIM59 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-105 amino acids from the N-terminal region of human TRIM59.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(TRIM59 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the TRIM59 antibody detected the TRIM59 protein (arrow).)
Western Blot (WB)
(TRIM59 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the TRIM59 antibody detected the TRIM59 protein (arrow).)
Immunohistochemistry (IHC)
(TRIM59 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRIM59 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(TRIM59 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRIM59 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of TRIM59 Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of TRIM59 Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
Product Categories/Family for anti-TRIM59 antibody
References
Georgescu, S.P., et al. Mol. Endocrinol. 19(10):2491-2501(2005). Chang, R., et al. Gene 291 (1-2), 241-249 (2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,114 Da
NCBI Official Full Name
tripartite motif-containing protein 59
NCBI Official Synonym Full Names
tripartite motif containing 59
NCBI Official Symbol
TRIM59
NCBI Official Synonym Symbols
MRF1; TSBF1; RNF104; TRIM57
NCBI Protein Information
tripartite motif-containing protein 59; tumor suppressor TSBF1; RING finger protein 104; tumor suppressor TSBF-1; tripartite motif-containing 57; tripartite motif-containing 59
UniProt Protein Name
Tripartite motif-containing protein 59
Protein Family
UniProt Gene Name
TRIM59
UniProt Synonym Gene Names
RNF104; TRIM57; TSBF1
UniProt Entry Name
TRI59_HUMAN
Uniprot Description
TRIM59: Belongs to the TRIM/RBCC family.
Protein type: Ubiquitin conjugating system; Membrane protein, integral
Chromosomal Location of Human Ortholog: 3q25.33
Cellular Component: endoplasmic reticulum; integral to membrane
Molecular Function: zinc ion binding
Biological Process: negative regulation of I-kappaB kinase/NF-kappaB cascade
Research Articles on TRIM59
Similar Products
Product Notes
The TRIM59 trim59 (Catalog #AAA6358518) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRIM59, NT (TRIM59, RNF104, TRIM57, TSBF1, Tripartite motif-containing protein 59, RING finger protein 104, Tumor suppressor TSBF-1) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRIM59 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Western Blot (WB). IF: 1:10-50 FLISA: 1:1,000 IHC: 1:10-50 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TRIM59 trim59 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIM59, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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