Western Blot (WB)
(TRIM50 Antibody (C-term) western blot analysis in Jurkat cell line lysates (15ug/lane).This demonstrates the TRIM50 antibody detected the TRIM50 protein (arrow).)
Rabbit anti-Human TRIM50 Polyclonal Antibody | anti-TRIM50 antibody
TRIM50 Antibody (C-term)
Gene Names
TRIM50; TRIM50A
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TRIM50; Polyclonal Antibody; TRIM50 Antibody (C-term); E3 ubiquitin-protein ligase TRIM50; 632-; Tripartite motif-containing protein 50; TRIM50A; anti-TRIM50 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TRIM50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 354-383 amino acids from the C-terminal region of human TRIM50.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
354-383
Sequence Length
486
Applicable Applications for anti-TRIM50 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(TRIM50 Antibody (C-term) western blot analysis in Jurkat cell line lysates (15ug/lane).This demonstrates the TRIM50 antibody detected the TRIM50 protein (arrow).)
Western Blot (WB)
(TRIM50 Antibody (C-term) western blot analysis in Jurkat cell line lysates (15ug/lane).This demonstrates the TRIM50 antibody detected the TRIM50 protein (arrow).)
Immunohistochemistry (IHC)
(TRIM50 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TRIM50 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(TRIM50 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TRIM50 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(TRIM50 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(TRIM50 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-TRIM50 antibody
TRIM50 belongs to the TRIM/RBCC family. It contains 1 B box-type zinc finger, 1 B30.2/SPRY domain and 1 RING-type zinc finger. TRIM50 is an E3 ubiquitin-protein ligase.
Product Categories/Family for anti-TRIM50 antibody
References
Micale, L., et al. Eur. J. Hum. Genet. 16(9):1038-1049(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54774
NCBI Official Full Name
E3 ubiquitin-protein ligase TRIM50 isoform b
NCBI Official Synonym Full Names
tripartite motif containing 50
NCBI Official Symbol
TRIM50
NCBI Official Synonym Symbols
TRIM50A
NCBI Protein Information
E3 ubiquitin-protein ligase TRIM50
UniProt Protein Name
E3 ubiquitin-protein ligase TRIM50
Protein Family
UniProt Gene Name
TRIM50
UniProt Synonym Gene Names
TRIM50A
UniProt Entry Name
TRI50_HUMAN
Uniprot Description
TRIM50: E3 ubiquitin-protein ligase. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin conjugating system; Ligase; EC 6.3.2.-; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 7q11.23
Cellular Component: cytoplasm
Molecular Function: zinc ion binding; ligase activity
Biological Process: metabolic process
Research Articles on TRIM50
Similar Products
Product Notes
The TRIM50 trim50 (Catalog #AAA9203136) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 354-383. The TRIM50 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRIM50 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the TRIM50 trim50 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIM50, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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