Rabbit TRIM5 Polyclonal Antibody | anti-TRIM5 antibody

Rabbit Polyclonal to Monkey TRIM5

Cat. Num. AAA247079

• Gene Names: TRIM5; RNF88; TRIM5alpha
• Reactivity: Human, Monkey
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: TRIM5; Polyclonal Antibody; Rabbit Polyclonal to Monkey TRIM5; Anti-TRIM5 Antibody IHC-plus; RNF88; RING finger protein 88; Tripartite motif containing 5; TRIM5alpha; Tripartite motif protein TRIM5; Tripartite motif-containing 5; Monkey TRIM5; anti-TRIM5 antibody

• Host: Rabbit
• Reactivity: Human, Monkey
• Clonality: Polyclonal
• Specificity: synthetic peptide corresponding to amino acids near the mid-region of rhesus monkey TRIM5a
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 135006516

• Applicable Applications for anti-TRIM5 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (5 ug/ml), WB (1 - 2 ug/ml)
• Target Species: Monkey
• Immunogen Description: Synthetic peptide from monkey TRIM5.
• Immunogen Type: Synthetic peptide
• Immunogen: TRIM5 antibody was raised against synthetic peptide from monkey TRIM5.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-TRIM5 antibody IHC of human prostate, stroma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-TRIM5 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of TRIM5a expression in human stomach (A), thymus (B), and uterus (C) cell lysate with TRIM5a antibody at 2 ug /ml.)

Related Product Information for anti-TRIM5 antibody

TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has six alternately spliced isoforms, the longest of which is the a variant which also contains a carboxy-terminal B30.2 (SPRY) domain (1).

Product Categories/Family for anti-TRIM5 antibody

Family: Tripartite Motif

NCBI and Uniprot Product Information

• NCBI GI #: 224589802
• NCBI GeneID: 85363
• NCBI Accession #: NC_000011.9
• NCBI GenBank Nucleotide #: NC_000011.9
• Molecular Weight: 29,677 Da
• NCBI Official Full Name: Homo sapiens chromosome 11, GRCh37.p13 Primary Assembly
• NCBI Official Synonym Full Names: tripartite motif containing 5
• NCBI Official Symbol: TRIM5
• NCBI Official Synonym Symbols: RNF88; TRIM5alpha
• NCBI Protein Information: tripartite motif-containing protein 5; ring finger protein 88; tripartite motif protein TRIM5; tripartite motif containing 5 transcript variant iota; tripartite motif containing 5 transcript variant kappa
• UniProt Protein Name: Tripartite motif-containing protein 5
• Protein Family: Tripartite motif-containing protein
• UniProt Gene Name: TRIM5
• UniProt Synonym Gene Names: RNF88
• UniProt Entry Name: TRIM5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2009]

Uniprot Description

TRIM5: Isoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity. Belongs to the TRIM/RBCC family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; Ubiquitin ligase; EC 6.3.2.19; EC 6.3.2.-; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: cytoplasm; cytosol

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; zinc ion binding; ubiquitin-protein ligase activity; pattern recognition receptor activity; ligase activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of MAPKKK cascade; viral reproduction; negative regulation of virion penetration into host cell; cytokine and chemokine mediated signaling pathway; innate immune response; positive regulation of transcription factor activity; pattern recognition receptor signaling pathway; activation of innate immune response; defense response to virus; regulation of lipopolysaccharide-mediated signaling pathway; activation of NF-kappaB transcription factor