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Western Blot (WB) (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-5: Hela cells, K562 cells, human fetal muscle tissue, A375 cells, hepg2 cells, Primary antibody: 862368(TRIM35 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)

Rabbit anti-Human TRIM35 Polyclonal Antibody | anti-TRIM35 antibody

TRIM35 Rabbit pAb

Gene Names
TRIM35; HLS5; MAIR
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
TRIM35; Polyclonal Antibody; TRIM35 Rabbit pAb; HLS5; MAIR; anti-TRIM35 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-TRIM35 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
Immunogen
Synthetic peptide of human TRIM35
Conjugation
Unconjugated
Molecular Function
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The function of this protein has not been identified.
Modification
Unmodified
Cell Position
Cytoplasm, Nucleus
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-5: Hela cells, K562 cells, human fetal muscle tissue, A375 cells, hepg2 cells, Primary antibody: 862368(TRIM35 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)

Western Blot (WB) (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-5: Hela cells, K562 cells, human fetal muscle tissue, A375 cells, hepg2 cells, Primary antibody: 862368(TRIM35 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 862368(TRIM35 Antibody) at dilution 1/25. (Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 862368(TRIM35 Antibody) at dilution 1/25. (Original magnification: ×200))
Product Categories/Family for anti-TRIM35 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
56kDa
NCBI Official Full Name
tripartite motif-containing protein 35 isoform 1
NCBI Official Synonym Full Names
tripartite motif containing 35
NCBI Official Symbol
TRIM35
NCBI Official Synonym Symbols
HLS5; MAIR
NCBI Protein Information
tripartite motif-containing protein 35
UniProt Protein Name
Tripartite motif-containing protein 35
UniProt Gene Name
TRIM35
UniProt Synonym Gene Names
HLS5; KIAA1098

NCBI Description

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The function of this protein has not been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

TRIM35: May play a role as a tumor suppressor. Implicated in the cell death mechanism. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 8p21.2

Cellular Component: cytoplasm; nucleus

Biological Process: apoptosis; innate immune response; negative regulation of mitotic cell cycle; positive regulation of apoptosis

Research Articles on TRIM35

Similar Products

Product Notes

The TRIM35 trim35 (Catalog #AAA4758559) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRIM35 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRIM35 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the TRIM35 trim35 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIM35, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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