Goat TRIM3 Polyclonal Antibody | anti-TRIM3 antibody

Goat Polyclonal to Human TRIM3

Cat. Num. AAA242587

• Gene Names: TRIM3; BERP; HAC1; RNF22; RNF97
• Reactivity: Bovine, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Rabbit, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: TRIM3; Polyclonal Antibody; Goat Polyclonal to Human TRIM3; Anti-TRIM3 Antibody (N-Terminus) IHC-plus; BERP; Brain expressed ring finger; Tripartite motif containing 3; Tripartite motif protein TRIM3; RING finger protein 97; RNF22; RNF97; HAC1; RING finger protein 22; Tripartite motif-containing 3; Human TRIM3; anti-TRIM3 antibody

• Host: Goat
• Reactivity: Bovine, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Rabbit, Rat
• Clonality: Polyclonal
• Specificity: Human TRIM3. Both variants (NP_006449.2; NP_150594.2;) represent the same protein. This antibody is expected to recognise all three human isoforms of this protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 744

• Applicable Applications for anti-TRIM3 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:64000), IHC-P (5 ug/ml), WB (0.1 - 1 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide AKREDSPGPEVQP-C from the N-terminus of human TRIM3 (NP_006449.2; NP_150594.2). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Rabbit (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: TRIM3 antibody was raised against synthetic peptide AKREDSPGPEVQP-C from the N-terminus of human TRIM3 (NP_006449.2; NP_150594.2). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Rabbit (100%).
• Antigen Modification: N-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-TRIM3 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Antibody staining (1 ug/ml) of Mouse Brain (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.)

Product Categories/Family for anti-TRIM3 antibody

Family: Tripartite Motif

NCBI and Uniprot Product Information

• NCBI GI #: 32454739
• NCBI GeneID: 10612
• NCBI Accession #: NP_006449.2
• NCBI GenBank Nucleotide #: NM_006458.3
• UniProt Accession #: O75382; Q4V9L4; Q9C038; Q9C039; B7Z5E6; F5H2Q8
• Molecular Weight: 67,726 Da
• NCBI Official Full Name: tripartite motif-containing protein 3 isoform 1
• NCBI Official Synonym Full Names: tripartite motif containing 3
• NCBI Official Symbol: TRIM3
• NCBI Official Synonym Symbols: BERP; HAC1; RNF22; RNF97
• NCBI Protein Information: tripartite motif-containing protein 3; RING finger protein 97; ring finger protein 22; brain expressed ring finger; tripartite motif-containing 3; tripartite motif protein TRIM3; brain-expressed RING finger protein
• UniProt Protein Name: Tripartite motif-containing protein 3
• Protein Family: Tripartite motif-containing protein
• UniProt Gene Name: TRIM3
• UniProt Synonym Gene Names: BERP; RNF22; RNF97
• UniProt Entry Name: TRIM3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic filaments. It is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin V-mediated cargo transport. Alternatively spliced transcript variants encoding the same isoform have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

TRIM3: Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Belongs to the TRIM/RBCC family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Cytoskeletal

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: early endosome; cytoplasm

Molecular Function: protein C-terminus binding; protein binding; zinc ion binding; ubiquitin-protein ligase activity

Biological Process: nervous system development; protein transport; protein ubiquitination