Rabbit anti-Human TRIM27 Polyclonal Antibody | anti-TRIM27 antibody

TRIM27 Rabbit pAb

Cat. Num. AAA4754513

• Gene Names: TRIM27; RFP; RNF76
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: TRIM27; Polyclonal Antibody; TRIM27 Rabbit pAb; RFP; RNF76; anti-TRIM27 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: -20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

• Applicable Applications for anti-TRIM27 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Fusion protein of human TRIM27
• Conjugation: Unconjugated
• Molecular Function: This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein.
• Modification: Unmodified
• Cell Position: Cytoplasm, Endosome, Nucleus
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-3: Human testis tissue, 293T and A172 cell lysates, Primary antibody: 123342(TRIM27 Antibody) at dilution 1/600, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 123342(TRIM27 Antibody) at dilution 1/65. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 123342(TRIM27 Antibody) at dilution 1/65. (Original magnification: ×200))

Product Categories/Family for anti-TRIM27 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 15488900
• NCBI GeneID: 5987
• UniProt Accession #: P14373; Q5RJA8; Q5ST26; Q6LA73; Q6NXR9; Q9BZY6; Q9UJL3; A2BE15
• Molecular Weight: 41,439 Da
• NCBI Official Full Name: Homo sapiens tripartite motif-containing 27, mRNA
• NCBI Official Synonym Full Names: tripartite motif containing 27
• NCBI Official Symbol: TRIM27
• NCBI Official Synonym Symbols: RFP; RNF76
• NCBI Protein Information: zinc finger protein RFP
• UniProt Protein Name: Zinc finger protein RFP
• Protein Family: Zinc finger protein
• UniProt Gene Name: TRIM27
• UniProt Synonym Gene Names: RFP; RNF76
• UniProt Entry Name: TRI27_HUMAN

NCBI Description

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. [provided by RefSeq, Jul 2008]