Rabbit anti-Human TRIM16 Polyclonal Antibody | anti-TRIM16 antibody

TRIM16, NT (TRIM16, EBBP, Tripartite motif-containing protein 16, Estrogen-responsive B box protein) (PE)

Cat. Num. AAA6358175

• Gene Names: TRIM16; EBBP
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TRIM16; Polyclonal Antibody; NT (TRIM16; EBBP; Tripartite motif-containing protein 16; Estrogen-responsive B box protein) (PE); Estrogen-responsive B box protein; anti-TRIM16 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-TRIM16 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TRIM16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-67 amino acids from the N-terminal region of human TRIM16.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TRIM16 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the TRIM16 antibody detected the TRIM16 protein (arrow).)

Related Product Information for anti-TRIM16 antibody

This gene was identified as an estrogen and anti-estrogen regulated gene in epithelial cells stably expressing estrogen receptor. The protein encoded by this gene contains two B box domains and a coiled-coiled region that are characteristic of the B box zinc finger protein family. The proteins of this family have been reported to be involved in a variety of biological processes including cell growth, differentiation and pathogenesis. Expression of this gene was detected in most tissues. Its function, however, has not yet been determined.

Product Categories/Family for anti-TRIM16 antibody

Antibodies; Transcription Factors; TRIM (Tripartite Motif)

References

Raif, A., et al. Cancer Lett. 277(1):82-90(2009). Olsen, J.V., et al. Cell 127(3):635-648(2006). Munding, C., et al. Cell Death Differ. 13(11):1938-1949(2006). Cheung, B.B., et al. J. Biol. Chem. 281(26):18246-18256(2006). Gwinn, M.R., et al. Environ. Health Perspect. 113(8):1046-1051(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 48255913
• NCBI GeneID: 10626
• NCBI Accession #: NP_006461.3
• NCBI GenBank Nucleotide #: NM_006470.3
• UniProt Accession #: O95361; Q6IAL8; Q7Z6I2; Q96BE8; Q96J43
• Molecular Weight: 63,955 Da
• NCBI Official Full Name: tripartite motif-containing protein 16
• NCBI Official Synonym Full Names: tripartite motif containing 16
• NCBI Official Symbol: TRIM16
• NCBI Official Synonym Symbols: EBBP
• NCBI Protein Information: tripartite motif-containing protein 16; tripartite motif-containing 16; estrogen-responsive B box protein
• UniProt Protein Name: Tripartite motif-containing protein 16
• Protein Family: Tripartite motif-containing protein
• UniProt Gene Name: TRIM16
• UniProt Synonym Gene Names: EBBP
• UniProt Entry Name: TRI16_HUMAN

NCBI Description

This gene was identified as an estrogen and anti-estrogen regulated gene in epithelial cells stably expressing estrogen receptor. The protein encoded by this gene contains two B box domains and a coiled-coiled region that are characteristic of the B box zinc finger protein family. The proteins of this family have been reported to be involved in a variety of biological processes including cell growth, differentiation and pathogenesis. Expression of this gene was detected in most tissues. Its function, however, has not yet been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TRIM16: May play a role in the regulation of keratinocyte differentiation. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: PML body; cytoplasm

Molecular Function: protein binding; NACHT domain binding; DNA binding; zinc ion binding; interleukin-1 binding

Biological Process: response to organophosphorus; response to retinoic acid; positive regulation of transcription, DNA-dependent; positive regulation of retinoic acid receptor signaling pathway; positive regulation of interleukin-1 beta secretion; positive regulation of keratinocyte differentiation