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Immunohistochemistry (IHC) (Anti-MID2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Goat TRIM1 / MID2 Polyclonal Antibody | anti-TRIM1 antibody

Goat Polyclonal to Human TRIM1 / MID2

Gene Names
MID2; FXY2; RNF60; TRIM1; MRX101
Reactivity
Gibbon, Gorilla, Human, Monkey, Mouse
Predicted Reactivity: Rat, Bat, Dog, Horse, Pig, Chicken (at least 90% immunogen sequence identity)
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Immunoaffinity Purified
Synonyms
TRIM1 / MID2; Polyclonal Antibody; Goat Polyclonal to Human TRIM1 / MID2; Anti-TRIM1 / MID2 Antibody (C-Terminus) IHC-plus; MID2; FXY2; Midin-2; Midline-2; Midin 2; Midline 2; Midline defect 2; RNF60; RING finger protein 60; TRIM1; Tripartite motif protein 1; Human TRIM1; anti-TRIM1 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Gibbon, Gorilla, Human, Monkey, Mouse
Predicted Reactivity: Rat, Bat, Dog, Horse, Pig, Chicken (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Specificity
Human MID2. This antibody is expected to recognise both human isoforms of this protein.
Purity/Purification
Immunoaffinity Purified
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
735
Applicable Applications for anti-TRIM1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA (1:32000), IHC-P (5 ug/ml), WB (1:32000, 1 - 3 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
Target Species
Human
Immunogen Description
Synthetic peptide QESPYVSGMKTCH from the C-terminus of human MID2 (NP_036348.2; NP_438112.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%).
Immunogen Type
Synthetic peptide
Immunogen
TRIM1 / MID2 antibody was raised against synthetic peptide QESPYVSGMKTCH from the C-terminus of human MID2 (NP_036348.2; NP_438112.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%); Mouse, Rat, Dog, Bat, Elephant, Panda, Horse, Pig, Opossum, Turkey, Chicken (92%); Hamster, Rabbit, Zebrafish, Stickleback (85%).
Antigen Modification
C-Terminus
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-MID2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC) (Anti-MID2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Antibody (1 ug/ml) staining of mouse heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.)

Western Blot (WB) (Antibody (1 ug/ml) staining of mouse heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
79,865 Da
NCBI Official Full Name
probable E3 ubiquitin-protein ligase MID2 isoform 1
NCBI Official Synonym Full Names
midline 2
NCBI Official Symbol
MID2
NCBI Official Synonym Symbols
FXY2; RNF60; TRIM1; MRX101
NCBI Protein Information
probable E3 ubiquitin-protein ligase MID2; midin 2; midin-2; midline-2; midline defect 2; RING finger protein 60; tripartite motif protein 1; tripartite motif-containing protein 1
UniProt Protein Name
Probable E3 ubiquitin-protein ligase MID2
UniProt Gene Name
MID2
UniProt Synonym Gene Names
FXY2; RNF60; TRIM1
UniProt Entry Name
TRIM1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009]

Uniprot Description

MID2: is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009]

Protein type: Microtubule-binding; EC 6.3.2.-; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: Xq22.3

Cellular Component: microtubule; cytoplasm

Molecular Function: protein homodimerization activity; zinc ion binding; protein heterodimerization activity; microtubule binding; phosphoprotein binding; ligase activity

Biological Process: negative regulation of viral transcription; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of virion penetration into host cell; protein ubiquitination; innate immune response; positive regulation of transcription factor activity; activation of NF-kappaB transcription factor

Disease: Mental Retardation, X-linked 101

Research Articles on TRIM1

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Product Notes

The TRIM1 mid2 (Catalog #AAA241947) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Polyclonal to Human TRIM1 / MID2 reacts with Gibbon, Gorilla, Human, Monkey, Mouse Predicted Reactivity: Rat, Bat, Dog, Horse, Pig, Chicken (at least 90% immunogen sequence identity) and may cross-react with other species as described in the data sheet. AAA Biotech's TRIM1 / MID2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA (1:32000), IHC-P (5 ug/ml), WB (1:32000, 1 - 3 ug/ml) Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the TRIM1 mid2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIM1 / MID2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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