Rabbit anti-Human, Mouse TRIB3 Polyclonal Antibody | anti-TRIB3 antibody

TRIB3, CT (Tribbles Homolog 3, Neuronal Cell Death Inducible Putative Kinase, p65-interacting Inhibitor of NF-kappa-B, SINK, TRB-3, C20orf97, NIPK, SKIP3, TRB3) (FITC)

Cat. Num. AAA6503589

• Gene Names: TRIB3; NIPK; SINK; TRB3; SKIP3; C20orf97
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: TRIB3; Polyclonal Antibody; CT (Tribbles Homolog 3; Neuronal Cell Death Inducible Putative Kinase; p65-interacting Inhibitor of NF-kappa-B; SINK; TRB-3; C20orf97; NIPK; SKIP3; TRB3) (FITC); anti-TRIB3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NPK. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-TRIB3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human NPK (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-NPK Pab is used in Western blot to detect NPK in mouse skeletal muscle tissue lysate.)

Product Categories/Family for anti-TRIB3 antibody

Antibodies; Protein Kinases

References

1. Wu, M., et al., J. Biol. Chem. 278(29):27072-27079 (2003). 2. Du, K., et al., Science 300(5625):1574-1577 (2003). 3. Bowers, A.J., et al., Oncogene 22(18):2823-2835 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 28201830
• NCBI GeneID: 57761
• UniProt Accession #: Q96RU7; Q53GU4; Q53ZW7; Q6I9Y9; Q8TAI6; Q9H5M8; Q9NUD2
• Molecular Weight: 358
• NCBI Official Full Name: Tribbles homolog 3
• NCBI Official Synonym Full Names: tribbles pseudokinase 3
• NCBI Official Symbol: TRIB3
• NCBI Official Synonym Symbols: NIPK; SINK; TRB3; SKIP3; C20orf97
• NCBI Protein Information: tribbles homolog 3; TRB-3; p65-interacting inhibitor of NF-kappaB; p65-interacting inhibitor of NF-kappa-B; neuronal cell death inducible putative kinase; neuronal cell death-inducible putative kinase
• UniProt Protein Name: Tribbles homolog 3
• Protein Family: Tribbles
• UniProt Gene Name: TRIB3
• UniProt Synonym Gene Names: C20orf97; NIPK; SKIP3; TRB3; TRB-3
• UniProt Entry Name: TRIB3_HUMAN

NCBI Description

The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. Differential promoter usage and alternate splicing result in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

TRB3: a Ca(2)/calmodulin-dependent protein kinase of the Trbl family. Induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. May negatively regulate the cell survival kinase AKT1.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CAMK; CAMK group; Trbl family

Chromosomal Location of Human Ortholog: 20p13-p12.2

Cellular Component: nucleoplasm; plasma membrane; cytosol; nucleus

Molecular Function: protein binding; protein kinase inhibitor activity; ubiquitin protein ligase binding; ubiquitin-protein ligase regulator activity; kinase activity; transcription corepressor activity; protein kinase binding; ATP binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; transcription, DNA-dependent; nerve growth factor receptor signaling pathway; positive regulation of protein binding; cellular lipid metabolic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of fat cell differentiation; protein amino acid phosphorylation; cellular response to insulin stimulus; regulation of MAP kinase activity; positive regulation of ubiquitin-protein ligase activity; insulin receptor signaling pathway; negative regulation of protein kinase activity; innate immune response; negative regulation of transcription, DNA-dependent; negative regulation of fatty acid biosynthetic process