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Western Blot (WB) ((0.3ug/ml) staining of Human Bone Marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat TRIB1/SKIP1 Polyclonal Antibody | anti-TRIB1 antibody

Goat anti-TRIB1/SKIP1 Antibody

Gene Names
TRIB1; C8FW; GIG2; TRB1; GIG-2; SKIP1; TRB-1
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
TRIB1/SKIP1; Polyclonal Antibody; Goat anti-TRIB1/SKIP1 Antibody; Goat Anti-Trib1 (mouse) (aa304-317) Antibody; TRIB1; tribbles homolog 1 (Drosophila); C8FW; GIG2; SKIP1; G-protein-coupled receptor induced protein; phosphoprotein regulated by mitogenic pathways; TRIB1 antibody; tribbles homolog 1 (Drosophila) antibody; C8FW antibody; GIG2 antibody; SKIP1 antibody; G-protein-coupled receptor induced protein antibody; phosphoprotein regulated by mitogenic pathways antibody; TRIB1 / SKIP1; anti-TRIB1 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
KHYQDKIRPYIQLPS
Sequence Length
372
Applicable Applications for anti-TRIB1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 40kDa band observed in Human Bone Marrow lysates (calculated MW of 41. 0kDa according to NP_079471.1). Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence C-KHYQDKIRPYIQLPS, from the internal region of the protein sequence according to NP_079471.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of Human Bone Marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0.3ug/ml) staining of Human Bone Marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,473 Da
NCBI Official Full Name
tribbles homolog 1 isoform 1
NCBI Official Synonym Full Names
tribbles pseudokinase 1
NCBI Official Symbol
TRIB1
NCBI Official Synonym Symbols
C8FW; GIG2; TRB1; GIG-2; SKIP1; TRB-1
NCBI Protein Information
tribbles homolog 1
UniProt Protein Name
Tribbles homolog 1
Protein Family
UniProt Gene Name
TRIB1
UniProt Synonym Gene Names
TRB-1; GIG-2
UniProt Entry Name
TRIB1_HUMAN

Uniprot Description

TRB1: Interacts with MAPK kinases and regulates activation of MAP kinases. May not display kinase activity. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.

Protein type: Kinase, protein; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); CAMK group; Trbl family

Chromosomal Location of Human Ortholog: 8q24.13

Cellular Component: cytoplasm; nucleus

Molecular Function: ATP binding; mitogen-activated protein kinase kinase binding; protein binding; protein kinase activity; protein kinase inhibitor activity; transcription factor binding; ubiquitin protein ligase binding; ubiquitin-protein ligase regulator activity

Biological Process: JNK cascade; negative regulation of lipopolysaccharide-mediated signaling pathway; negative regulation of neutrophil differentiation; negative regulation of protein kinase activity; negative regulation of smooth muscle cell migration; negative regulation of smooth muscle cell proliferation; negative regulation of transcription factor activity; positive regulation of eosinophil differentiation; positive regulation of macrophage differentiation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein amino acid phosphorylation; regulation of MAP kinase activity; response to lipopolysaccharide

Research Articles on TRIB1

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Product Notes

The TRIB1 trib1 (Catalog #AAA423331) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-TRIB1/SKIP1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's TRIB1/SKIP1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 40kDa band observed in Human Bone Marrow lysates (calculated MW of 41. 0kDa according to NP_079471.1). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the TRIB1 trib1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KHYQDKIRPY IQLPS. It is sometimes possible for the material contained within the vial of "TRIB1/SKIP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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