Rabbit anti-Human TRIB1 Polyclonal Antibody | anti-TRIB1 antibody

TRIB1, CT (Tribbles Homolog 1, TRB-1, SKIP1, G-protein-coupled Receptor-induced Protein 2, GIG-2, TRIB1, C8FW, GIG2) (AP)

Cat. Num. AAA6503553

• Gene Names: TRIB1; C8FW; GIG2; TRB1; GIG-2; SKIP1; TRB-1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: TRIB1; Polyclonal Antibody; CT (Tribbles Homolog 1; TRB-1; SKIP1; G-protein-coupled Receptor-induced Protein 2; GIG-2; C8FW; GIG2) (AP); anti-TRIB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TRIB1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-TRIB1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human TRIB1 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-TRIB1 Pab (AP7726b) in T47D cell line lysate (35ug/lane). TRIB1(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-TRIB1 antibody

Antibodies; G Protein Receptors

References

1. Kiss-Toth, E., et al., J. Biol. Chem. 279(41):42703-42708 (2004). 2. Wu, M., et al., J. Biol. Chem. 278(29):27072-27079 (2003). 3. Wilkin, F., et al., Eur. J. Biochem. 248(3):660-668 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 13399328
• NCBI GeneID: 10221
• NCBI Accession #: NP_079471
• NCBI GenBank Nucleotide #: NM_025195
• UniProt Accession #: Q96RU8; O15180; Q9H2Y8; B4DMM6; C5HU08
• Molecular Weight: 23,473 Da
• NCBI Official Full Name: tribbles homolog 1 isoform 1
• NCBI Official Synonym Full Names: tribbles pseudokinase 1
• NCBI Official Symbol: TRIB1
• NCBI Official Synonym Symbols: C8FW; GIG2; TRB1; GIG-2; SKIP1; TRB-1
• NCBI Protein Information: tribbles homolog 1; G-protein-coupled receptor induced protein; G-protein-coupled receptor-induced gene 2 protein; G-protein-coupled receptor-induced protein 2; TRIB1; phosphoprotein regulated by mitogenic pathways; tribbles-like protein 1
• UniProt Protein Name: Tribbles homolog 1
• Protein Family: Tribbles
• UniProt Gene Name: TRIB1
• UniProt Synonym Gene Names: TRB-1; GIG-2
• UniProt Entry Name: TRIB1_HUMAN

Uniprot Description

TRB1: Interacts with MAPK kinases and regulates activation of MAP kinases. May not display kinase activity. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CAMK; CAMK group; Trbl family

Chromosomal Location of Human Ortholog: 8q24.13

Cellular Component: cytoplasm; nucleus

Molecular Function: protein kinase inhibitor activity; mitogen-activated protein kinase kinase binding; ubiquitin protein ligase binding; ubiquitin-protein ligase regulator activity; transcription factor binding; ATP binding; protein kinase activity

Biological Process: negative regulation of lipopolysaccharide-mediated signaling pathway; negative regulation of smooth muscle cell proliferation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; regulation of MAP kinase activity; negative regulation of transcription factor activity; JNK cascade; negative regulation of protein kinase activity; response to lipopolysaccharide; negative regulation of smooth muscle cell migration; protein amino acid phosphorylation