Rabbit Treacle Polyclonal Antibody | anti-Q55 antibody

Treacle (Q55) Polyclonal Antibody

Cat. Num. AAA3002105

• Gene Names: TCOF1; TCS; MFD1; TCS1; treacle
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Treacle; Polyclonal Antibody; Treacle (Q55) Polyclonal Antibody; Treacle protein; Treacher Collins syndrome protein; TCOF1; anti-Q55 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Treacle (Q55) polyclonal antibody detects endogenous levels of Treacle protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1411

• Applicable Applications for anti-Q55 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 22-73 of Human Treacle.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Treacle (Q55) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Treacle (Q55) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-Q55 antibody

Treacle is a serine/alanine-rich 1, 411 amino acid nuclear phosphoprotein that interacts with upstream binding factor (UBF) and affects transcription of the ribosomal DNA gene. Treacle is also involved in early embryonic development, particularly in the craniofacial complex, and may play a role in nucleolarcytoplasmic transport. The Treacle protein contains three domains with unique N- and C-termini and a large central repeat domain. Mutations in TCOF1, the gene that encodes for Treacle, cause Treacher Collins-Franceschetti syndrome (TCS), a disorder characterized by defects in craniofacial development. Symptoms of TCS include conductive hearing loss, hypoplasia of the mandible and maxilla, downward sloping palpebral fissures and cleft palate.

NCBI and Uniprot Product Information

• NCBI GI #: 57164975
• NCBI GeneID: 6949
• NCBI Accession #: NP_000347.2
• NCBI GenBank Nucleotide #: NP_000347.2
• UniProt Accession #: Q13428
• Molecular Weight: ~ 152kDa
• NCBI Official Full Name: treacle protein isoform b
• NCBI Official Synonym Full Names: treacle ribosome biogenesis factor 1
• NCBI Official Symbol: TCOF1
• NCBI Official Synonym Symbols: TCS; MFD1; TCS1; treacle
• NCBI Protein Information: treacle protein
• UniProt Protein Name: Treacle protein
• Protein Family: Treacle protein
• UniProt Gene Name: TCOF1
• UniProt Entry Name: TCOF_HUMAN

NCBI Description

This gene encodes a nucleolar protein with a LIS1 homology domain. The protein is involved in ribosomal DNA gene transcription through its interaction with upstream binding factor (UBF). Mutations in this gene have been associated with Treacher Collins syndrome, a disorder which includes abnormal craniofacial development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

treacle: May be involved in nucleolar-cytoplasmic transport. May play a fundamental role in early embryonic development, particularly in development of the craniofacial complex. May participate in certain stages of ribosome biogenesis. Defects in TCOF1 are the cause of Treacher Collins syndrome type 1 (TCS1). It is a form of Treacher Collins syndrome, a disorder of craniofacial development. Treacher Collins syndrome is characterized by a combination of bilateral downward slanting of the palpebral fissures, colobomas of the lower eyelids with a paucity of eyelashes medial to the defect, hypoplasia of the facial bones, cleft palate, malformation of the external ears, atresia of the external auditory canals, and bilateral conductive hearing loss. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; RNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; transporter activity

Biological Process: transport; skeletal development; transcription of nuclear rRNA large RNA polymerase I transcript

Disease: Treacher Collins Syndrome 1