Rabbit TRB-3 Polyclonal Antibody | anti-TRIB3 antibody

TRB-3 (Tribbles Homolog 3, Neuronal Cell death-inducible Putative Kinase, SINK, p65-interacting Inhibitor of NF-kappaB)

Cat. Num. AAA6001176

• Gene Names: TRIB3; NIPK; SINK; TRB3; SKIP3; C20orf97
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: TRB-3; Polyclonal Antibody; TRB-3 (Tribbles Homolog 3; Neuronal Cell death-inducible Putative Kinase; SINK; p65-interacting Inhibitor of NF-kappaB); Anti -TRB-3 (Tribbles Homolog 3; anti-TRIB3 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Specificity: Recognizes human TRB-3. Detects a band of ~28kD by Western blot (predicted 39kD).
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.

• Applicable Applications for anti-TRIB3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Immunohistochemistry, Western Blot
• Immunogen: Synthetic peptide corresponding to aa 112-125 (A112RLPPHKHVARPTE125) of human TRB-3.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Immunohistochemical staining of of human tissue using MBS6001176 at 1:2000 dilution. 1: Immunoperoxidase staining (cytoplasmic) of formalin-fixed, paraffin-embedded human salivary glands (200x, brown color).)

Western Blot (WB)

(Western blot analysis of human tissue lysate using MBS6001176 at 1:2000 dilution. 1. Human spleen tissue lysate (10ug). 2. Human liver tissue lysate (10ug) 3. Human colon tissue lysate (10ug) 4. Human Thpok (His-tagged) (negative control).)

Related Product Information for anti-TRIB3 antibody

Tribbles homolog 3 (TRB-3) is a pseudokinase, shown to act as a potent negative regulator of PPAR?, a master regulator of adipocyte differentiation, and to control adipogenesis. It disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. It interacts also with the NF-kappaB transactivator p65 RELA. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells.

Product Categories/Family for anti-TRIB3 antibody

Antibodies; Abs to Protein Kinase Inhibitors

NCBI and Uniprot Product Information

• NCBI GI #: 31542265
• NCBI GeneID: 57761
• NCBI Accession #: NP_066981.2
• NCBI GenBank Nucleotide #: NM_021158.3
• UniProt Accession #: Q96RU7; Q53GU4; Q53ZW7; Q6I9Y9; Q8TAI6; Q9H5M8; Q9NUD2
• Molecular Weight: 39,578 Da
• NCBI Official Full Name: tribbles homolog 3
• NCBI Official Synonym Full Names: tribbles homolog 3 (Drosophila)
• NCBI Official Symbol: TRIB3
• NCBI Official Synonym Symbols: NIPK; SINK; TRB3; SKIP3; C20orf97
• NCBI Protein Information: tribbles homolog 3; TRB-3; p65-interacting inhibitor of NF-kappaB; p65-interacting inhibitor of NF-kappa-B; neuronal cell death inducible putative kinase; neuronal cell death-inducible putative kinase
• UniProt Protein Name: Tribbles homolog 3
• Protein Family: Tribbles
• UniProt Gene Name: TRIB3
• UniProt Synonym Gene Names: C20orf97; NIPK; SKIP3; TRB3; TRB-3
• UniProt Entry Name: TRIB3_HUMAN

NCBI Description

The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. [provided by RefSeq, Jul 2008]

Uniprot Description

TRB3: a Ca(2)/calmodulin-dependent protein kinase of the Trbl family. Induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. May negatively regulate the cell survival kinase AKT1.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; CAMK group; Trbl family

Chromosomal Location of Human Ortholog: 20p13-p12.2

Cellular Component: nucleoplasm; plasma membrane; cytosol; nucleus

Molecular Function: protein binding; protein kinase inhibitor activity; ubiquitin-protein ligase regulator activity; ubiquitin protein ligase binding; kinase activity; transcription corepressor activity; protein kinase binding; ATP binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; transcription, DNA-dependent; nerve growth factor receptor signaling pathway; positive regulation of protein binding; cellular lipid metabolic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of fat cell differentiation; protein amino acid phosphorylation; cellular response to insulin stimulus; regulation of MAP kinase activity; positive regulation of ubiquitin-protein ligase activity; insulin receptor signaling pathway; innate immune response; negative regulation of protein kinase activity; negative regulation of transcription, DNA-dependent; negative regulation of fatty acid biosynthetic process