Rabbit TRAP220/MED1 Polyclonal Antibody | anti-TRAP220/MED1 antibody

Phospho-TRAP220/MED1 (Thr1032) Antibody

Cat. Num. AAA9614303

• Gene Names: MED1; PBP; CRSP1; RB18A; TRIP2; PPARBP; CRSP200; DRIP205; DRIP230; PPARGBP; TRAP220
• Reactivity: Human, Mouse; Predicted Reactivity: Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (86%)
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: TRAP220/MED1; Polyclonal Antibody; Phospho-TRAP220/MED1 (Thr1032) Antibody; Activator-recruited cofactor 205kDa component; ARC205; CRSP1; CRSP200; DRIP205; DRIP230; MED1; MED1_HUMAN; Mediator complex subunit 1; Mediator of RNA polymerase II transcription subunit 1; p53 regulatory protein RB18A; PBP; Peroxisome proliferator-activated receptor-binding protein; PPAR binding protein; PPAR-binding protein; PPARBP; PPARGBP; RB18A; Thyroid hormone receptor-associated protein complex 220kDa component; Thyroid receptor-interacting protein 2; TR-interacting protein 2; Trap220; TRIP-2; TRIP2; Vitamin D receptor-interacting protein complex component DRIP205; anti-TRAP220/MED1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse; Predicted Reactivity: Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (86%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-TRAP220/MED1 (Thr1032) Antibody detects endogenous levels of TRAP220/MED1 only when phosphorylated at Thr1032.; Tissue Specificity: Ubiquitously expressed.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-TRAP220/MED1 antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
• Application Notes: WB: 1:1000-3000; IF/ICC: 1:100-1:500; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human TRAP220/MED1 around the phosphorylation site of Thr1032.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylation increases its interaction with PSIP1.
• Subunit Structure: Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. This subunit specifically interacts with a number of nuclear receptors in a ligand-dependent fashion including AR, ESR1, ESR2, PPARA, PPARG, RORA, RXRA, RXRG, THRA, THRB and VDR. Interacts with CTNNB1, GABPA, GLI3, PPARGC1A and TP53. Interacts with YWHAH. Interacts with CLOCK; this interaction requires the presence of THRAP3 (By similarity). Interacts with GATA1 and CCAR1. Interacts with NR4A3 (By similarity). Interacts (via IBM motif) with PSIP1 (via IBD domain); phosphorylation increases its affinity for PSIP1.
• Similarity: Belongs to the Mediator complex subunit 1 family.
• Subcellular Location: Nucleus.; Note: A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of PPARBP (Phospho-Thr1032) using Na3VO4 treated K562 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(Staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Related Product Information for anti-TRAP220/MED1 antibody

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells.

NCBI and Uniprot Product Information

• NCBI GI #: 28559039
• NCBI GeneID: 5469
• NCBI Accession #: NP_004765.2
• NCBI GenBank Nucleotide #: NM_004774.3
• UniProt Accession #: Q15648; O43810; O75447; Q6P9H7; Q6PK58; Q9HD39; A2RRQ6
• Molecular Weight: 168,478 Da
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 1
• NCBI Official Synonym Full Names: mediator complex subunit 1
• NCBI Official Symbol: MED1
• NCBI Official Synonym Symbols: PBP; CRSP1; RB18A; TRIP2; PPARBP; CRSP200; DRIP205; DRIP230; PPARGBP; TRAP220
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 1; ARC205; TRIP-2; PPAR binding protein; PPAR-binding protein; PPARG binding protein; TR-interacting protein 2; p53 regulatory protein RB18A; thyroid receptor interacting protein 2; thyroid receptor-inte
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 1
• UniProt Gene Name: MED1
• UniProt Synonym Gene Names: ARC205; CRSP1; CRSP200; DRIP205; DRIP230; PBP; PPARBP; PPARGBP; RB18A; TRAP220; TRIP2; ARC205; PBP; PPAR-binding protein; Trap220; TR-interacting protein 2; TRIP-2
• UniProt Entry Name: MED1_HUMAN

NCBI Description

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. [provided by RefSeq, Jul 2008]

Uniprot Description

MED1: a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. Also is a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. May regulates p53-dependent apoptosis. Is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. Two splice-variant isoforms have been described.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: nucleoplasm; membrane; nucleolus; Srb-mediator complex; nucleus; chromatin

Molecular Function: ligand-dependent nuclear receptor binding; peroxisome proliferator activated receptor binding; retinoic acid receptor binding; chromatin DNA binding; transcription coactivator activity; receptor activity; transcription factor binding; thyroid hormone receptor coactivator activity; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; vitamin D receptor binding; transcription cofactor activity; LBD domain binding; estrogen receptor binding; chromatin binding; thyroid hormone receptor binding; nuclear hormone receptor binding

Biological Process: fat cell differentiation; lactation; lens development in camera-type eye; embryonic placenta development; erythrocyte development; regulation of cell cycle; positive regulation of transcription, DNA-dependent; positive regulation of estrogen receptor signaling pathway; positive regulation of mammary gland epithelial cell proliferation; cell morphogenesis; enucleate erythrocyte development; negative regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process; androgen biosynthetic process; embryonic hindlimb morphogenesis; angiogenesis; positive regulation of keratinocyte differentiation; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; regulation of transcription from RNA polymerase I promoter; organ regeneration; positive regulation of protein import into nucleus, translocation; monocyte differentiation; embryonic hemopoiesis; thyroid hormone generation; liver development; mRNA transcription from RNA polymerase II promoter; keratinocyte differentiation; negative regulation of neuron differentiation; androgen receptor signaling pathway; embryonic heart tube development; gene expression; positive regulation of transcription from RNA polymerase II promoter; brain development; negative regulation of apoptosis