Western Blot (WB)
(Figure 1. Western blot analysis of TRAP/CD40L/CD40LG using anti-TRAP/CD40L/CD40LG antibody (MBS1753397).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human HL-60 whole cell lysatesLane 2: human K562 whole cell lysatesLane 3: human Raji whole cell lysatesLane 4: human Jurkat whole cell lysatesLane 5: rat RH35 whole cell lysatesLane 6: mouse spleen tissue lysatesLane 7: mouse ANA-1 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-TRAP/CD40L/CD40LG antigen affinity purified polyclonal antibody (Catalog # MBS1753397) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for TRAP/CD40L/CD40LG at approximately 36KD. The expected band size for TRAP/CD40L/CD40LG is at 36KD. )
Rabbit TRAP/CD40L/CD40LG Polyclonal Antibody | anti-CD40LG antibody
Anti-TRAP/CD40L/CD40LG Antibody
; anti-CD40LG antibody
FC/FACS/FCM: 1-3ug/1x106 cells|Human, Rat|
Direct ELISA: 0.1-0.5ug/ml|Human|
Western Blot (WB)
(Figure 1. Western blot analysis of TRAP/CD40L/CD40LG using anti-TRAP/CD40L/CD40LG antibody (MBS1753397).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human HL-60 whole cell lysatesLane 2: human K562 whole cell lysatesLane 3: human Raji whole cell lysatesLane 4: human Jurkat whole cell lysatesLane 5: rat RH35 whole cell lysatesLane 6: mouse spleen tissue lysatesLane 7: mouse ANA-1 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-TRAP/CD40L/CD40LG antigen affinity purified polyclonal antibody (Catalog # MBS1753397) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for TRAP/CD40L/CD40LG at approximately 36KD. The expected band size for TRAP/CD40L/CD40LG is at 36KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of TRAP/CD40L/CD40LG using anti-TRAP/CD40L/CD40LG antibody (MBS1753397).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human HL-60 whole cell lysatesLane 2: human K562 whole cell lysatesLane 3: human Raji whole cell lysatesLane 4: human Jurkat whole cell lysatesLane 5: rat RH35 whole cell lysatesLane 6: mouse spleen tissue lysatesLane 7: mouse ANA-1 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-TRAP/CD40L/CD40LG antigen affinity purified polyclonal antibody (Catalog # MBS1753397) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for TRAP/CD40L/CD40LG at approximately 36KD. The expected band size for TRAP/CD40L/CD40LG is at 36KD. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of C6 cells using anti-TRAP/CD40L/CD40LG antibody (MBS1753397).Overlay histogram showing C6 cells stained with MBS1753397 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAP/CD40L/CD40LG Antibody (MBS1753397, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of C6 cells using anti-TRAP/CD40L/CD40LG antibody (MBS1753397).Overlay histogram showing C6 cells stained with MBS1753397 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAP/CD40L/CD40LG Antibody (MBS1753397, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of human PBMC cells using anti-TRAP/CD40L/CD40LG antibody (MBS1753397).Overlay histogram showing human PBMC cells stained with MBS1753397 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAP/CD40L/CD40LG Antibody (MBS1753397, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of human PBMC cells using anti-TRAP/CD40L/CD40LG antibody (MBS1753397).Overlay histogram showing human PBMC cells stained with MBS1753397 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAP/CD40L/CD40LG Antibody (MBS1753397, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
2. Cipollone,F.;Ferri,C.;Desideri,G.;Paloscia,L.;Materazzo,G.;Mascellanti,M.;Fazia,M.;Iezzi,A.;Cuccurullo,C.;Pini,B.;Bucci,M.;Santucci,A.;Cuccurullo,F.; Mezzetti,A. :Preprocedural level of soluble CD40L is predictive of enhanced inflammatory response and restenosis after coronary angioplasty. Circulation 108: 2776-2782, 2003.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]
Uniprot Description
CD40LG: Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1); also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. Belongs to the tumor necrosis factor family.
Protein type: Membrane protein, integral; Cell adhesion
Chromosomal Location of Human Ortholog: Xq26
Cellular Component: extracellular space; integral to plasma membrane; integral to membrane; plasma membrane; external side of plasma membrane
Molecular Function: CD40 receptor binding; cytokine activity; tumor necrosis factor receptor binding
Biological Process: B cell proliferation; platelet activation; leukocyte adhesion; regulation of immune response; positive regulation of interleukin-12 production; immunoglobulin secretion; B cell differentiation; isotype switching; inflammatory response; regulation of immunoglobulin secretion; negative regulation of apoptosis
Disease: Immunodeficiency With Hyper-igm, Type 1
Research Articles on CD40LG
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Product Notes
The CD40LG cd40lg (Catalog #AAA1753397) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TRAP/CD40L/CD40LG Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TRAP/CD40L/CD40LG can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Human, Mouse, Rat| FC/FACS/FCM: 1-3ug/1x106 cells|Human, Rat| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the CD40LG cd40lg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRAP/CD40L/CD40LG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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