Rabbit anti-Human Transketolase Polyclonal Antibody | anti-TK antibody

Transketolase (TK, TKT, TKT1) (Biotin)

Cat. Num. AAA6396612

• Gene Names: TKT; TK; TKT1; HEL107
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Transketolase; Polyclonal Antibody; Transketolase (TK; TKT; TKT1) (Biotin); EC=2.2.1.1; anti-TK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TKT.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TK antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human TKT, aa1-623 (NP_001055.1).
• Immunogen Sequence: MESYHKPDQQKLQALKDTANRLRISSIQATTAAGSGHPTSCCSAAEIMAVLFFHTMRYKSQDPRNPHNDRFVLSKGHAAPILYAVWAEAGFLAEAELLNLRKISSDLDGHPVPKQAFTDVATGSLGQGLGAACGMAYTGKYFDKASYRVYCLLGDGELSEGSVWEAMAFASIYKLDNLVAILDINRLGQSDPAPLQHQMDIYQKRCEAFGWHAIIVDGHSVEELCKAFGQAKHQPTAIIAKTFKGRGITGVEDKESWHGKPLPKNMAEQIIQEIYSQIQSKKKILATPPQEDAPSVDIANIRMPSLPSYKVGDKIATRKAYGQALAKLGHASDRIIALDGDTKNSTFSEIFKKEHPDRFIECYIAEQNMVSIAVGCATRNRTVPFCSTFAAFFTRAFDQIRMAAISESNINLCGSHCGVSIGEDGPSQMALEDLAMFRSVPTSTVFYPSDGVATEKAVELAANTKGICFIRTSRPENAIIYNNNEDFQVGQAKVVLKSKDDQVTVIGAGVTLHEALAAAELLKKEKINIRVLDPFTIKPLDRKLILDSARATKGRILTVEDHYYEGGIGEAVSSAVVGEPGITVTHLAVNRVPRSGKPAELLKMFGIDRDAIAQAVRGLITKA
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TKT rabbit polyclonal antibody. Western Blot analysis of TKT expression in human pancreas.)

Western Blot (WB)

(TKT rabbit polyclonal antibody. Western Blot analysis of TKT expression in Jurkat.)

Western Blot (WB)

(Western Blot analysis of TKT expression in transfected 293T cell line by TKT polyclonal antibody. Lane 1: TKT transfected lysate (67.9kD). Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-TK antibody

Antibodies; Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 4507521
• NCBI GeneID: 7086
• NCBI Accession #: NP_001055.1
• NCBI GenBank Nucleotide #: NM_001064.1
• UniProt Accession #: P29401; Q8TBA3; Q96HH3; A8K089; B4DE31; E7EPA7
• Molecular Weight: 68,814 Da
• NCBI Official Full Name: transketolase isoform 1
• NCBI Official Synonym Full Names: transketolase
• NCBI Official Symbol: TKT
• NCBI Official Synonym Symbols: TK; TKT1; HEL107
• NCBI Protein Information: transketolase; epididymis luminal protein 107
• UniProt Protein Name: Transketolase
• Protein Family: Transketolase
• UniProt Gene Name: TKT
• UniProt Synonym Gene Names: TK
• UniProt Entry Name: TKT_HUMAN

NCBI Description

This gene encodes a thiamine-dependent enzyme which plays a role in the channeling of excess sugar phosphates to glycolysis in the pentose phosphate pathway. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

TKT: Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate. Homodimer. Belongs to the transketolase family.

Protein type: Transferase; EC 2.2.1.1; Carbohydrate Metabolism - pentose phosphate pathway

Chromosomal Location of Human Ortholog: 3p14.3

Cellular Component: nucleoplasm; peroxisome; cytosol; nucleus; vesicle

Molecular Function: transketolase activity; protein homodimerization activity; metal ion binding; cofactor binding

Biological Process: pentose-phosphate shunt; regulation of growth; carbohydrate metabolic process; energy reserve metabolic process; glyceraldehyde-3-phosphate biosynthetic process; pentose-phosphate shunt, non-oxidative branch; pathogenesis; xylulose biosynthetic process

Disease: Wernicke-korsakoff Syndrome