Rabbit anti-Human, Mouse TPX2 Polyclonal Antibody | anti-TPX2 antibody

Rabbit Polyclonal (IgG) to Human TPX2

Cat. Num. AAA244670

• Gene Names: TPX2; DIL2; p100; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: TPX2; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human TPX2; Anti-TPX2 Antibody (aa301-350) IHC-plus; C20orf2; DIL-2; DIL2; HCA519; GD:C20orf1; p100; Protein fls353; Targeting protein for Xklp2; C20orf1; FLS353; HCTP4; REPP86; Human TPX2; anti-TPX2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TPX2 Antibody detects endogenous levels of total TPX2 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 747

• Applicable Applications for anti-TPX2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: IHC-P (1:100); WB (1:500 - 1:1000); Peptide Enzyme-Linked Immunosorbent Assay (1:1000); ; (optimal dilution to be determined by the researcher)
• Target Species: Human
• Immunogen: TPX2 antibody was raised against synthetic peptide from human DIL-2. (AA Range: 301-350)
• Antigen Modification: aa301-350
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-TPX2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-TPX2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Western blot of extracts from Jurkat cells, using TPX2 Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-TPX2 antibody

TPX2 is a spindle assembly factor, required for normal assembly of mitotic spindles, for normal assembly of microtubules during apoptosis, and for chromatin and/or kinetochore dependent microtubule nucleation. TPX2 mediates AURKA localization to spindle microtubules, activates AURKA by promoting its autophosphorylation at 'Thr-288', and protects this residue against dephosphorylation.

NCBI and Uniprot Product Information

• NCBI GI #: 20127519
• NCBI GeneID: 22974
• NCBI Accession #: NP_036244.2
• NCBI GenBank Nucleotide #: NM_012112.4
• UniProt Accession #: Q9ULW0; Q96RR5; Q9H1R4; Q9NRA3; Q9UFN9; Q9UL00; Q9Y2M1
• Molecular Weight: Western Blot: Predicted MW 85 kDa
• NCBI Official Full Name: targeting protein for Xklp2
• NCBI Official Synonym Full Names: TPX2, microtubule-associated
• NCBI Official Symbol: TPX2
• NCBI Official Synonym Symbols: DIL2; p100; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1
• NCBI Protein Information: targeting protein for Xklp2; protein fls353; TPX2, microtubule-associated, homolog; differentially expressed in lung cells; TPX2, microtubule-associated protein homolog; preferentially expressed in colorectal cancer; hepatocellular carcinoma-associated antigen 519; restricted expression proliferation associated protein 100; restricted expression proliferation-associated protein 100; differentially expressed in cancerous and non-cancerous lung cells 2
• UniProt Protein Name: Targeting protein for Xklp2
• Protein Family: Targeting protein
• UniProt Gene Name: TPX2
• UniProt Synonym Gene Names: C20orf1; C20orf2; DIL2; HCA519; DIL-2; p100
• UniProt Entry Name: TPX2_HUMAN

Uniprot Description

TPX2: a microtubule-associated protein required for meiotic and mitotic spindle assembly. Targets a plus end-directed motor (XKLP2) to the minus ends of microtubules in the mitotic spindle. Required for microtubule nucleation around chromosomes in response to the local generation of RanGTP. Binds to and activates Aurora A kinase and targets it to the mitotic spindle.

Protein type: Microtubule-binding; Cell cycle regulation

Chromosomal Location of Human Ortholog: 20q11.2

Cellular Component: axon hillock; nucleoplasm; microtubule cytoskeleton; spindle pole; microtubule; cytoplasm; spindle; nucleus

Molecular Function: protein binding; GTP binding; protein kinase binding; ATP binding

Biological Process: mitosis; cell proliferation; cell division; apoptosis; activation of protein kinase activity