Western Blot (WB)
((1ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat TPTE/PTEN2 Polyclonal Antibody | anti-TPTE antibody
Goat anti-TPTE/PTEN2 Antibody
Gene Names
TPTE; CT44; PTEN2
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
TPTE/PTEN2; Polyclonal Antibody; Goat anti-TPTE/PTEN2 Antibody; TPTE; PTEN2; transmembrane phosphatase with tensin homology; tensin; putative protein-tyrosine phosphatase; TPTE antibody; PTEN2 antibody; transmembrane phosphatase with tensin homology antibody; putative protein-tyrosine phosphatase antibody; TPTE / PTEN2; anti-TPTE antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
ERRTDKTHSEKFQ
Sequence Length
551
Applicable Applications for anti-TPTE antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 50-55kDa band observed in Human Testis lysates (calculated MW of 60.2kDa according to NP_954869.1). Recommended concentration: 1-3ug/ml.
Western Blot: Approx 50-55kDa band observed in Human Testis lysates (calculated MW of 60.2kDa according to NP_954869.1). Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence C-ERRTDKTHSEKFQ, from the internal region of the protein sequence according to NP_954870.2; NP_954868.1; NP_954869.1.
Epitope
Internal region
Note
This antibody is expected to recognise all reported isoforms (NP_954870.2, NP_954868.1 and NP_954869.1). It may cross-react with the almost identical TPTE2 (GeneID 93492; NP_570141.2, NP_954864.1, NP_954863.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((1ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49,344 Da
NCBI Official Full Name
putative tyrosine-protein phosphatase TPTE isoform alpha
NCBI Official Synonym Full Names
transmembrane phosphatase with tensin homology
NCBI Official Symbol
TPTE
NCBI Official Synonym Symbols
CT44; PTEN2
NCBI Protein Information
putative tyrosine-protein phosphatase TPTE
UniProt Protein Name
Putative tyrosine-protein phosphatase TPTE
Protein Family
UniProt Gene Name
TPTE
UniProt Synonym Gene Names
CT44
UniProt Entry Name
TPTE_HUMAN
NCBI Description
This gene encodes a PTEN-related tyrosine phosphatase which may play a role in the signal transduction pathways of the endocrine or spermatogenic function of the testis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
Uniprot Description
TPTE: Could be involved in signal transduction. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Cancer Testis Antigen (CTA); Motility/polarity/chemotaxis; Membrane protein, multi-pass; Membrane protein, integral; EC 3.1.3.48; Receptor protein phosphatase, tyrosine
Chromosomal Location of Human Ortholog: 21p11
Cellular Component: integral to membrane
Molecular Function: protein tyrosine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity
Biological Process: protein amino acid dephosphorylation; signal transduction
Research Articles on TPTE
Similar Products
Product Notes
The TPTE tpte (Catalog #AAA420300) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-TPTE/PTEN2 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's TPTE/PTEN2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 50-55kDa band observed in Human Testis lysates (calculated MW of 60.2kDa according to NP_954869.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the TPTE tpte for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ERRTDKTHSE KFQ. It is sometimes possible for the material contained within the vial of "TPTE/PTEN2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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