Rabbit anti-Mouse TPSAB1 Polyclonal Antibody | anti-TPSAB1 antibody

TPSAB1, CT (TPSAB1, TPS1, TPS2, TPSB1, Tryptase alpha/beta-1, Tryptase I, Tryptase alpha-1) (MaxLight 650)

Cat. Num. AAA6357733

• Gene Names: TPSAB1; TPS1; TPS2; TPSB1
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: TPSAB1; Polyclonal Antibody; CT (TPSAB1; TPS1; TPS2; TPSB1; Tryptase alpha/beta-1; Tryptase I; Tryptase alpha-1) (MaxLight 650); Tryptase alpha-1; anti-TPSAB1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse TPSAB1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-TPSAB1 antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 249-275 in the C-terminal region of human TPSAB1, UniProt Accession #Q15661. Species sequence homology: human
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse liver tissue lysates (35ug/lane) usingMBS6006934. This demonstrates MBS6006934 detected the TPSAB1 protein (arrow).)

Related Product Information for anti-TPSAB1 antibody

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.

Product Categories/Family for anti-TPSAB1 antibody

Antibodies; Enzymes; Protease (Proteinase; Peptidase)

References

1. Lee, J.W., et al. Dig. Dis. Sci. 55(10):2922-2928(2010). 2. Trivedi, N.N., et al. J. Allergy Clin. Immunol. 124(5):1099-1105(2009). 3. Radhakrishnan, Y., et al. Biol. Reprod. 81(4):647-656(2009). 4. Schiemann, F., et al. J. Immunol. 183(4):2223-2231(2009). 5. Wei, Z.Y., et al. Fa Yi Xue Za Zhi 25(3):164-167(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 13775595
• NCBI GeneID: 7177
• NCBI Accession #: NP_003285
• NCBI GenBank Nucleotide #: NM_003294.3
• UniProt Accession #: Q15661; P15157; Q15663; Q6B052; Q9H2Y4; Q9H2Y5; Q9UQI1; D2E6R9; D2E6S1
• Molecular Weight: 30.1 kDa (270aa)
• NCBI Official Full Name: tryptase alpha/beta-1
• NCBI Official Synonym Full Names: tryptase alpha/beta 1
• NCBI Official Symbol: TPSAB1
• NCBI Official Synonym Symbols: TPS1; TPS2; TPSB1
• NCBI Protein Information: tryptase alpha/beta-1
• UniProt Protein Name: Tryptase alpha/beta-1
• Protein Family: Tryptase
• UniProt Gene Name: TPSAB1
• UniProt Synonym Gene Names: TPS1; TPS2; TPSB1; Tryptase-1
• UniProt Entry Name: TRYB1_HUMAN

NCBI Description

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]

Uniprot Description

TPSAB1: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Belongs to the peptidase S1 family. Tryptase subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.21.59; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: extracellular matrix; extracellular space; extracellular region

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; defense response; proteolysis