Rabbit TPSAB1 Polyclonal Antibody | anti-TPSAB1 antibody

TPSAB1 Rabbit Polyclonal

Cat. Num. AAA7605136

• Gene Names: TPSAB1; TPS1; TPS2; TPSB1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: TPSAB1; Polyclonal Antibody; TPSAB1 Rabbit Polyclonal; anti-TPSAB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 275

• Applicable Applications for anti-TPSAB1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: Tryptase alpha/beta 1
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human appendix using MBS7605136 (TPSAB1 antibody) at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of (-20 degree C Ethanol) fixed HepG2 cells using MBS7605136 (TPSAB1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L))

Western Blot (WB)

(mouse liver tissue were subjected to SDS PAGE followed by western blot with MBS7605136 (TPSAB1 antibody) at dilution of 1:800)

NCBI and Uniprot Product Information

• NCBI GI #: 13775595
• NCBI GeneID: 7177
• NCBI Accession #: NP_003285.2
• NCBI GenBank Nucleotide #: NM_003294.3
• Molecular Weight: 29,533 Da
• NCBI Official Full Name: tryptase alpha/beta-1
• NCBI Official Synonym Full Names: tryptase alpha/beta 1
• NCBI Official Symbol: TPSAB1
• NCBI Official Synonym Symbols: TPS1; TPS2; TPSB1
• NCBI Protein Information: tryptase alpha/beta-1
• UniProt Protein Name: Tryptase alpha/beta-1
• Protein Family: Tryptase
• UniProt Gene Name: TPSAB1
• UniProt Synonym Gene Names: TPS1; TPS2; TPSB1; Tryptase-1
• UniProt Entry Name: TRYB1_HUMAN

NCBI Description

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]

Uniprot Description

TPSAB1: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Belongs to the peptidase S1 family. Tryptase subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.59; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: extracellular matrix; extracellular region; extracellular space

Molecular Function: protein binding; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: defense response; extracellular matrix disassembly; proteolysis