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Immunohistochemistry (IHC) (Anti-TPRA1 / GPR175 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Rabbit TPRA1 / GPR175 Polyclonal Antibody | anti-TPRA1 antibody

Rabbit Polyclonal to Human TPRA1 / GPR175

Gene Names
TPRA1; GPR175; TPRA40; TMEM227
Reactivity
Bat, Gibbon, Bovine, Dog, Gorilla, Hamster, Horse, Human, Mouse, Pig, Rat
Predicted Reactivity: Monkey, Rabbit, Zebrafish (at least 90% immunogen sequence identity)
Applications
Immunohistochemistry
Purity
Immunoaffinity Purified
Synonyms
TPRA1 / GPR175; Polyclonal Antibody; Rabbit Polyclonal to Human TPRA1 / GPR175; Anti-TPRA1 / GPR175 Antibody (Cytoplasmic Domain) IHC-plus; TPRA1; G protein-coupled receptor 175; Transmembrane protein 227; TMEM227; TPRA40; GPR175; Human TPRA1; anti-TPRA1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Bat, Gibbon, Bovine, Dog, Gorilla, Hamster, Horse, Human, Mouse, Pig, Rat
Predicted Reactivity: Monkey, Rabbit, Zebrafish (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Specificity
Human TPRA1 / GPR175. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS, 0.1% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
293
Applicable Applications for anti-TPRA1 antibody
Immunohistochemistry (IHC - Paraffin)
Application Notes
IHC-P (10 - 20 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
Target Species
Human
Immunogen Description
Synthetic 17 amino acid peptide from 2nd cytoplasmic domain of human TPRA1 / GPR175.
Immunogen Type
Synthetic peptide
Immunogen
TPRA1 / GPR175 antibody was raised against synthetic 17 amino acid peptide from 2nd cytoplasmic domain of human TPRA1 / GPR175. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Dog, Bovine, Bat, Hamster, Elephant, Panda, Horse, Pig, Platypus (100%); Monkey, Rabbit, Opossum, Turkey, Lizard, Zebrafish (94%); Chicken, Seabass, Salmon, Stickleback (88%).
Antigen Modification
Cytoplasmic Domain
Preparation and Storage
Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC)

(Anti-TPRA1 / GPR175 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC) (Anti-TPRA1 / GPR175 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-TPRA1 / GPR175 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC) (Anti-TPRA1 / GPR175 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-TPRA1 / GPR175 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC) (Anti-TPRA1 / GPR175 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-TPRA1 / GPR175 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC) (Anti-TPRA1 / GPR175 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-TPRA1 / GPR175 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC) (Anti-TPRA1 / GPR175 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC) (Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE))
Related Product Information for anti-TPRA1 antibody
GPR175 is an Orphan-U GPCR with an unknown ligand. LOC51210 has been implicated in the effects of ischemic hypoxia and reoxygenation.
Product Categories/Family for anti-TPRA1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32,117 Da
NCBI Official Synonym Full Names
transmembrane protein, adipocyte asscociated 1
NCBI Official Symbol
TPRA1
NCBI Official Synonym Symbols
GPR175; TPRA40; TMEM227
NCBI Protein Information
transmembrane protein adipocyte-associated 1
UniProt Protein Name
Transmembrane protein adipocyte-associated 1
UniProt Gene Name
TPRA1
UniProt Synonym Gene Names
GPR175; TMEM227
UniProt Entry Name
TPRA1_HUMAN

Uniprot Description

GPR175: Belongs to the UPF0359 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 3q21.2

Cellular Component: integral to membrane

Molecular Function: G-protein coupled receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; lipid metabolic process; aging

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Product Notes

The TPRA1 tpra1 (Catalog #AAA240912) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human TPRA1 / GPR175 reacts with Bat, Gibbon, Bovine, Dog, Gorilla, Hamster, Horse, Human, Mouse, Pig, Rat Predicted Reactivity: Monkey, Rabbit, Zebrafish (at least 90% immunogen sequence identity) and may cross-react with other species as described in the data sheet. AAA Biotech's TPRA1 / GPR175 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin). IHC-P (10 - 20 ug/ml) Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the TPRA1 tpra1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TPRA1 / GPR175, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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