Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TPPP3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5min.)
Rabbit TPPP3 Polyclonal Antibody | anti-TPPP3 antibody
TPPP3 Polyclonal Antibody
Gene Names
TPPP3; p20; CGI-38; TPPP/p20; p25gamma
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Synonyms
TPPP3; Polyclonal Antibody; TPPP3 Polyclonal Antibody; p20; CGI-38; TPPP/p20; p25gamma; anti-TPPP3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
176
Applicable Applications for anti-TPPP3 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IHC: 1:50 - 1:200
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human TPPP3
Calculated Molecular Weight
18kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TPPP3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5min.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TPPP3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5min.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human gastric cancer using TPPP3 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human gastric cancer using TPPP3 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human colon carcinoma using TPPP3 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human colon carcinoma using TPPP3 antibody at dilution of 1:100 (40x lens).)
Product Categories/Family for anti-TPPP3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
176
NCBI Official Full Name
Tubulin polymerization-promoting protein family member 3
NCBI Official Synonym Full Names
tubulin polymerization-promoting protein family member 3
NCBI Official Symbol
TPPP3
NCBI Official Synonym Symbols
p20; CGI-38; TPPP/p20; p25gamma
NCBI Protein Information
tubulin polymerization-promoting protein family member 3; brain specific protein
UniProt Protein Name
Tubulin polymerization-promoting protein family member 3
Protein Family
UniProt Gene Name
TPPP3
UniProt Entry Name
TPPP3_HUMAN
Uniprot Description
CGI-38: Binds tubulin and has microtubule bundling activity. May play a role in cell proliferation and mitosis. Belongs to the TPPP family.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 16q22.1
Cellular Component: microtubule; cytoplasm
Molecular Function: tubulin binding
Biological Process: microtubule bundle formation
Research Articles on TPPP3
Similar Products
Product Notes
The TPPP3 tppp3 (Catalog #AAA9125909) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TPPP3 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TPPP3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the TPPP3 tppp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TPPP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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