Rabbit anti-Human TPM4 Polyclonal Antibody | anti-TPM4 antibody

TPM4, NT (TPM4, Tropomyosin alpha-4 chain, TM30p1, Tropomyosin-4)

Cat. Num. AAA6012584

• Reactivity: Human
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: TPM4; Polyclonal Antibody; NT (TPM4; Tropomyosin alpha-4 chain; TM30p1; Tropomyosin-4); Anti -TPM4; anti-TPM4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-TPM4 antibody: ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Flow Cytometry: 1:10-50
• Immunogen: TPM4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-62 amino acids from the N-terminal region of human TPM4.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TPM4 Antibody (N-term) western blot analysis in U251,A549,Jurkat cell line lysates (35ug/lane).This demonstrates the TPM4 antibody detected the TPM4 protein (arrow).)

Flow Cytometry (FC/FACS)

(TPM4 Antibody (N-term) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TPM4 antibody

This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Product Categories/Family for anti-TPM4 antibody

Antibodies; Abs to Actin Related Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 223555975
• NCBI GeneID: 7171
• NCBI Accession #: NP_001138632.1
• NCBI GenBank Nucleotide #: NM_001145160.1
• UniProt Accession #: P67936; P07226; Q15659; Q5U0D9; Q9BU85; Q9H8Q3; Q9UCS1; Q9UCS2; Q9UCS3; Q9UCS4
• Molecular Weight: 28,522 Da
• NCBI Official Full Name: tropomyosin alpha-4 chain isoform 1
• NCBI Official Synonym Full Names: tropomyosin 4
• NCBI Official Symbol: TPM4
• NCBI Protein Information: tropomyosin alpha-4 chain; TM30p1; tropomyosin-4
• UniProt Protein Name: Tropomyosin alpha-4 chain
• Protein Family: Tropomyosin
• UniProt Gene Name: TPM4
• UniProt Entry Name: TPM4_HUMAN

NCBI Description

This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2009]

Uniprot Description

TPM4: a cytoskeletal protein that binds to actin filaments in muscle and nonmuscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In nonmuscle cells is implicated in stabilizing cytoskeleton actin filaments and endocytosis.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: cortical cytoskeleton; filamentous actin; cytoskeleton; focal adhesion; membrane; stress fiber; podosome; cytosol; muscle thin filament tropomyosin

Molecular Function: structural constituent of muscle; calcium ion binding; actin binding

Biological Process: osteoblast differentiation; muscle contraction; cell motility; muscle filament sliding