Rabbit TPM1 Polyclonal Antibody | anti-TPM1 antibody

TPM1 Polyclonal Antibody

Cat. Num. AAA127505

• Gene Names: TPM1; CMH3; TMSA; CMD1Y; LVNC9; C15orf13; HTM-alpha
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: TPM1; Polyclonal Antibody; TPM1 Polyclonal Antibody; CMH3; TMSA; CMD1Y; C15orf13; HTM-alpha; anti-TPM1 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 284

• Applicable Applications for anti-TPM1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
• Species reactivity/Cross reactivity: Human, Mouse, Rat, Zebrafish
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human Tropomyosin 1 (NP_001018008.1)
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using TPM1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells using TPM1 antibody. Blue: DAPI for nuclear staining.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin embedded human lung using Tropomyosin 1 antibody (MBS127505) at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin embedded human appendix using Tropomyosin 1 antibody (MBS127505) at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffinembedded human uterus using Tropomyosin 1 antibody (MBS127505) at dilution of 1:100 (40x lens).)

Related Product Information for anti-TPM1 antibody

Tropomyosin-1 (TPM1) belongs to the high molecular weight members of tropomyosin family (1,2). The protein exists in an alpha-helical coiled-coil conformation and binds multiple acting monomers in a tight manner to stabilize and regulate the actin filament (3). Tropomyosins fullfill functions in muscle and non-muscle cells. In muscle cells, tropomyosins associate with the troponin complex and play a central role in the calcium-dependent regulation of striated muscle contraction in vertebrates. In non-muscle cells, tropomyosins are implicated in the formation and stabilization of cytoskeletal actin filaments to ensure normal cellular processes (1,2). Mutations of tropomysin-1 have been reported as a cause of dilated cardiac myopathies (4). Tropomyosin-1 also functions as a tumor suppressor, and many malignant tumors demonstrate downregulation of tropomyosin-1 expression (5-8). Tropomyosin-1 is phosphorylated at Ser283 through the Erk/DAPK pathway, which promotes stress fiber formation in response to oxidative stress (9-10).

Product Categories/Family for anti-TPM1 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 136092
• NCBI GeneID: 7168
• UniProt Accession #: P09493; P09494; P10469; Q6DV89; Q6DV90; Q7Z6L8; Q86W64; Q96IK2; B7Z5T7; D9YZV2; D9YZV3; D9YZV8
• Molecular Weight: Observed MW: 36kDa; Calculated MW: 26kDa/28kDa/32kDa
• NCBI Official Full Name: Tropomyosin alpha-1 chain
• NCBI Official Synonym Full Names: tropomyosin 1 (alpha)
• NCBI Official Symbol: TPM1
• NCBI Official Synonym Symbols: CMH3; TMSA; CMD1Y; LVNC9; C15orf13; HTM-alpha
• NCBI Protein Information: tropomyosin alpha-1 chain; alpha-tropomyosin; sarcomeric tropomyosin kappa; cardiomyopathy, hypertrophic 3
• UniProt Protein Name: Tropomyosin alpha-1 chain
• Protein Family: Tropomyosin
• UniProt Gene Name: TPM1
• UniProt Synonym Gene Names: C15orf13; TMSA
• UniProt Entry Name: TPM1_HUMAN

NCBI Description

This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

Uniprot Description

TPM1: a cytoskeletal protein that binds to actin filaments in muscle and nonmuscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Defects in TPM3 are a cause of nemaline myopathy type 1 (NEM1). Three alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Actin-binding; Motor

Chromosomal Location of Human Ortholog: 15q22.1

Cellular Component: filamentous actin; sarcomere; cytoskeleton; stress fiber; cytosol; muscle thin filament tropomyosin

Molecular Function: structural constituent of cytoskeleton; structural constituent of muscle; cytoskeletal protein binding; actin binding

Biological Process: positive regulation of cell adhesion; wound healing; regulation of muscle contraction; in utero embryonic development; cytoskeleton organization and biogenesis; sarcomere organization; regulation of heart contraction; muscle filament sliding; muscle contraction; positive regulation of ATPase activity; positive regulation of stress fiber formation; ruffle organization and biogenesis; ventricular cardiac muscle morphogenesis; cell motility; negative regulation of cell migration; positive regulation of heart rate by epinephrine; cardiac muscle contraction

Disease: Cardiomyopathy, Dilated, 1y; Cardiomyopathy, Familial Hypertrophic, 3