Rabbit TORC2 Polyclonal Antibody | anti-TORC2 antibody

TORC2 Antibody

Cat. Num. AAA9613050

• Gene Names: CRTC2; TORC2; TORC-2
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Zebrafish (82%), Bovine (100%), Horse (100%), Rabbit (100%)
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: TORC2; Polyclonal Antibody; TORC2 Antibody; CREB regulated transcription coactivator 2; CREB-regulated transcription coactivator 2; CRTC2; CRTC2_HUMAN; RP11-422P24.6; TORC-2; torc2; Transducer of CREB protein 2; Transducer of regulated cAMP response element-binding protein; Transducer of regulated cAMP response element-binding protein (CREB) 2; Transducer of regulated cAMP response element-binding protein 2; Transducer of regulated CREB protein 2; anti-TORC2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Zebrafish (82%), Bovine (100%), Horse (100%), Rabbit (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: TORC2 Antibody detects endogenous levels of total TORC2.; Tissue Specificity: Most abundantly expressed in the thymus. Present in both B and T-lymphocytes. Highly expressed in HEK293T cells and in insulinomas. High levels also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels found in brain, colon, heart, kidney, prostate, small intestine and stomach. Weak expression in liver and pancreas.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-TORC2 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human TORC2, corresponding to a region within N-terminal amino acids.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylation/dephosphorylation states of Ser-171 are required for regulating transduction of CREB activity. CRTCs/TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation, is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs (SIK1 and SIK2) by LKB1. Following adenylyl cyclase activation, dephosphorylated at Ser-171 by PPP3CA/calcineurin A resulting in CRTC2 dissociation from 14-3-3 proteins and PPP3CA (By similarity). Both insulin and AMPK increase this phosphorylation of CRTC2 while glucagon suppresses it. Phosphorylation at Ser-274 by MARK2 is induced under low glucose conditions and dephosphorylated in response to glucose influx. Phosphorylation at Ser-274 promotes interaction with 14-3-3 proteins and translocation to the cytoplasm. Asymmetric dimethylation of arginine resisues by PRMT6 enhances the association of CRTC2 with CREB on the promoters of gluconeogenic genes.
• Subunit Structure: Binds, as a tetramer, through its N-terminal region, with the bZIP domain of CREB1. 'Arg-314' in the bZIP domain of CREB1 is essential for this interaction. Interaction, via its C-terminal, with TAF4, enhances recruitment of TAF4 to CREB1. Interacts with SIK2. Interacts with 14-3-3 proteins, YWHAB and YWHAG. Interacts (probably when phosphorylated at Ser-171) with YWHAE. Interacts with calmodulin-dependent catalytic subunit PPP3CA/calcineurin A. Interaction with COP1 mediates nuclear export and degradation of CRTC2 (By similarity). (Microbial infection) Interaction with the human T-cell leukemia virus type 1 (HTLV-1) Tax protein is essential for optimal transcription activation by Tax.
• Similarity: Belongs to the TORC family.
• Subcellular Location: Cytoplasm. Nucleus.; Note: Translocated from the nucleus to the cytoplasm on interaction of the phosphorylated form with 14-3-3 protein (PubMed:15454081). In response to cAMP levels and glucagon, relocated to the nucleus (PubMed:15454081).
• Preparation and Storage: Store at -20 degree C. Stable for 15 months from date of receipt.

Western Blot (WB)

(Western blot analysis of TORC2 antibody expression in A431 cells lysates.The lane on the left was treated with the antigen-specific peptide.)

Related Product Information for anti-TORC2 antibody

Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).

NCBI and Uniprot Product Information

• NCBI GI #: 32171215
• NCBI GeneID: 200186
• NCBI Accession #: NP_859066
• NCBI GenBank Nucleotide #: NM_181715.2
• UniProt Accession #: Q53ET0; Q6UUV8; Q7Z3X7; Q8N332
• Molecular Weight: Predicted: 67; 76 kDa; ; Observed: 70 kDa
• NCBI Official Full Name: CREB-regulated transcription coactivator 2
• NCBI Official Synonym Full Names: CREB regulated transcription coactivator 2
• NCBI Official Symbol: CRTC2
• NCBI Official Synonym Symbols: TORC2; TORC-2
• NCBI Protein Information: CREB-regulated transcription coactivator 2; transducer of regulated cAMP response element-binding protein (CREB) 2
• UniProt Protein Name: CREB-regulated transcription coactivator 2
• UniProt Gene Name: CRTC2
• UniProt Synonym Gene Names: TORC2; TORC-2; Transducer of CREB protein 2
• UniProt Entry Name: CRTC2_HUMAN

NCBI Description

This gene encodes a member of the transducers of regulated cAMP response element-binding protein activity family of transcription coactivators. These proteins promote the transcription of genes targeted by the cAMP response element-binding protein, and therefore play an important role in many cellular processes. Under basal conditions the encoded protein is phosphorylated by AMP-activated protein kinase or the salt-inducible kinases and is sequestered in the cytoplasm. Upon activation by elevated cAMP or calcium, the encoded protein translocates to the nucleus and increases target gene expression. Single nucleotide polymorphisms in this gene may increase the risk of type 2 diabetes. A pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2010]

Uniprot Description

TORC2: the transducer of regulated CREB protein 2 (TORC2) is a cAMP responsive coactivator that, in concert with LKB1 and AMPK, controls glucose homeostasis in the liver. Under fasting conditions, cytoplasmic TORC2 is transported to the nucleus where it binds to CREB and stimulates gluconeogenisis. Re-feeding and the ensuing insulin response inhibits gluconeogenic gene expression by promoting the phosphorylation, cytoplasmic export and degradation of TORC2. TORC2 is phosphorylated by the kinase QIK which in turn is activated by phosphorylation at Ser358 by insulin-activated Akt2. Phosphorylated TORC2 is subsequently ubiquitinylated at Lys 628 and degraded by the 26S proteasome. TORC2 protein levels and activity are increased in diabetes owing to a block in TORC2 phosphorylation, implicating this pathway in the pathogenesis of diabetes. The CREB/TORC2 regulatory axis controls the normal pattern of germinal center (GC) B cell gene activation/repression that promotes B cell development and circumvents GC lymphomagenesis.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding; cAMP response element binding protein binding

Biological Process: viral reproduction; transcription, DNA-dependent; activation of CREB transcription factor; positive regulation of transcription from RNA polymerase II promoter; glucose homeostasis; gluconeogenesis; protein homotetramerization