Rabbit anti-Human, Rat Topoisomerase II Polyclonal Antibody
Topoisomerase II (DNA) alpha (DNA Topoisomerase 2 alpha, DNA Topoisomerase II alpha Isozyme, TOP2, TOP2A, TP2A)
Reactivity
Human, Rat
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Topoisomerase II; Polyclonal Antibody; Topoisomerase II (DNA) alpha (DNA Topoisomerase 2 alpha; DNA Topoisomerase II alpha Isozyme; TOP2; TOP2A; TP2A); Anti -Topoisomerase II (DNA) alpha (DNA Topoisomerase 2 alpha; anti-Topoisomerase II antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human DNA topoisomerase 2-alpha at 174kD. Species Crossreactivity: rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitute with 100ul of sterile dH2O.
Applicable Applications for anti-Topoisomerase II antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1-2ug/ml
Dilution: Western Blot: 1-2ug/ml
Immunogen
Synthetic peptide corresponding to aa1466-1480, RKPSTSDDSDSNFEK to human DNA topoisomerase 2-alpha.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Topoisomerase II antibody
DNA topoisomerase 2 catalyzes the transient breaking and rejoining of double stranded DNA during events such as DNA synthesis and transcription. Two forms of this enzyme exist, alpha and beta. DNA topoisomerase 2 alpha (TOP2A) is localized to chromosome 17 and the beta gene is localized to chromosome 3. At least 4 isoforms of TOP2A are produced by alternate splicing.
Product Categories/Family for anti-Topoisomerase II antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
topoisomerase II
Similar Products
Product Notes
The Topoisomerase II (Catalog #AAA621221) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Topoisomerase II (DNA) alpha (DNA Topoisomerase 2 alpha, DNA Topoisomerase II alpha Isozyme, TOP2, TOP2A, TP2A) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Topoisomerase II can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1-2ug/ml. Researchers should empirically determine the suitability of the Topoisomerase II for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Topoisomerase II, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
