Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western blot analysis of 293T using Topo IIIalpha antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000)

Rabbit anti-Human, Mouse TOP3A Polyclonal Antibody | anti-TOP3A antibody

TOP3A Antibody

Gene Names
TOP3A; TOP3
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
TOP3A; Polyclonal Antibody; TOP3A Antibody; TOP3; DNA topoisomerase 3-alpha; DNA topoisomerase III alpha; anti-TOP3A antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid; In Phosphate Buffered Saline, 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Applicable Applications for anti-TOP3A antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA: 1:10000
Immunogen
Synthesized peptide derived from the Internal region of human Topo IIIalpha. at AA rangle: 350-430
Immunogen Species
Human
Conjugate
Non-conjugated
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blot analysis of 293T using Topo IIIalpha antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000)

Western Blot (WB) (Western blot analysis of 293T using Topo IIIalpha antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000)
Product Categories/Family for anti-TOP3A antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
112,372 Da
NCBI Official Full Name
DNA topoisomerase 3-alpha
NCBI Official Synonym Full Names
topoisomerase (DNA) III alpha
NCBI Official Symbol
TOP3A
NCBI Official Synonym Symbols
TOP3
NCBI Protein Information
DNA topoisomerase 3-alpha; topo III-alpha; DNA topoisomerase III alpha
UniProt Protein Name
DNA topoisomerase 3-alpha
Protein Family
UniProt Gene Name
TOP3A
UniProt Synonym Gene Names
TOP3
UniProt Entry Name
TOP3A_HUMAN

NCBI Description

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. [provided by RefSeq, Jul 2008]

Uniprot Description

TOP3A: a DNA topoisomerase. Reduces the number of supercoils in a highly negatively supercoiled DNA. Two alternatively spliced human isoforms have been described.

Protein type: EC 5.99.1.2; Isomerase

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: PML body; chromosome; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; DNA topoisomerase type I activity

Biological Process: meiosis; DNA topological change

Research Articles on TOP3A

Similar Products

Product Notes

The TOP3A top3a (Catalog #AAA7122728) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TOP3A Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TOP3A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000 ELISA: 1:10000. Researchers should empirically determine the suitability of the TOP3A top3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TOP3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.