Rabbit anti-Human TOM1L1 Polyclonal Antibody | anti-TOM1L1 antibody

TOM1L1, CT (TOM1L1, SRCASM, TOM1-like protein 1, Src-activating and signaling molecule protein, Target of Myb-like protein 1) (APC)

Cat. Num. AAA6357308

• Gene Names: TOM1L1; SRCASM; OK/KNS-CL.3
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TOM1L1; Polyclonal Antibody; CT (TOM1L1; SRCASM; TOM1-like protein 1; Src-activating and signaling molecule protein; Target of Myb-like protein 1) (APC); Target of Myb-like protein 1; anti-TOM1L1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-TOM1L1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TOM1L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444-474 amino acids from the C-terminal region of human TOM1L1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TOM1L1 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TOM1L1 antibody detected the TOM1L1 protein (arrow).)

Related Product Information for anti-TOM1L1 antibody

Probable adapter protein involved in signaling pathways. Interacts with the SH2 and SH3 domains of various signaling proteins when it is phosphorylated. May promotes FYN activation, possibly by disrupting intramolecular SH3-dependent interactions (By similarity).

Product Categories/Family for anti-TOM1L1 antibody

Antibodies; Signal Transduction

References

Qi, Y., et al. Anticancer Res. 30(9):3535-3539(2010). Liu, N.S., et al. EMBO J. 28(22):3485-3499(2009). Meulener, M.C., et al. J. Cutan. Pathol. 36(3):291-295(2009). Elmarghani, A., et al. Mediators Inflamm. 2009, 416298 (2009): Thalappilly, S., et al. Proteomics 8(15):3071-3081(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 191252812
• NCBI GeneID: 10040
• NCBI Accession #: NP_005477.2
• NCBI GenBank Nucleotide #: NM_005486.2
• UniProt Accession #: O75674; Q53G06; Q8N749
• Molecular Weight: 38,713 Da
• NCBI Official Full Name: TOM1-like protein 1
• NCBI Official Synonym Full Names: target of myb1 (chicken)-like 1
• NCBI Official Symbol: TOM1L1
• NCBI Official Synonym Symbols: SRCASM; OK/KNS-CL.3
• NCBI Protein Information: TOM1-like protein 1; target of myb1-like 1; target of myb 1-like 1; target of Myb-like protein 1; Src activating and signaling molecule; src-activating and signaling molecule protein
• UniProt Protein Name: TOM1-like protein 1
• Protein Family: TOM1-like protein
• UniProt Gene Name: TOM1L1
• UniProt Synonym Gene Names: SRCASM
• UniProt Entry Name: TM1L1_HUMAN

Uniprot Description

Srcasm: an adapter protein that is a src-family kinase substrate. When phosphorylated, it binds src-family kinases and targets them for lysosomal-dependent degradation. EGF triggers a transient association of phosphorylated Srcasm with EGFR via Grb2 and Shc initiating the endocytosis of the ligand-receptor complex. Interacts with the SH2 and SH3 domains of various signaling proteins when it is phosphorylated. Involved in multivesicular body (MVB) formation, viral egress from the cell, and cytokinesis. Belongs to the TOM1 family. Interacts with FYN, GRB2 and PIK3R1 when phosphorylated. The PTAP sequence in the GAT domain and the PSAP sequence in the C-terminal region are responsible for its interaction with the UEV domain of Tsg101, a component of the ESCRT-1 complex. Localizes to the multivesicular body (MVB) that is involved in targeting endosomal proteins for lysosomal degradation.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q23.2

Cellular Component: Golgi stack; lysosome; cytoplasm; endosome membrane; cytosol; endosome

Molecular Function: clathrin binding; protein binding; ubiquitin binding; SH3 domain binding; protein kinase activator activity; protein kinase binding

Biological Process: intracellular protein transport; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; negative regulation of mitosis; activation of protein kinase activity; signal transduction; positive regulation of protein amino acid autophosphorylation