Rabbit anti-Human TNFRSF25 Polyclonal Antibody | anti-TNFRSF25 antibody

TNFRSF25 Polyclonal Antibody

Cat. Num. AAA3007668

• Gene Names: TNFRSF25; DR3; TR3; DDR3; LARD; APO-3; TRAMP; WSL-1; GEF720; WSL-LR; PLEKHG5; TNFRSF12
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TNFRSF25; Polyclonal Antibody; TNFRSF25 Polyclonal Antibody; Tumor necrosis factor receptor superfamily member 25; Apo-3; Apoptosis-inducing receptor AIR; Apoptosis-mediating receptor DR3; Apoptosis-mediating receptor TRAMP; Death receptor 3; Lymphocyte-associated receptor of deat; LARD; Protein WSL; Protein WSL-1; APO3; DDR3; DR3; TNFRSF12; WSL; WSL1; UNQ455; PRO779; anti-TNFRSF25 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TNFRSF25 polyclonal antibody detects endogenous levels of TNFRSF25 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 372

• Applicable Applications for anti-TNFRSF25 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 250-300 of Human TNFRSF25.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of TNFRSF25 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:PC3 whole cell lysate (40ug) Lane4:U-87MG whole cell lysate (40ug))

Related Product Information for anti-TNFRSF25 antibody

The tumor necrosis factor receptor family, which includes TNF-RI, Fas, DR3, DR4, DR5, and DR6, plays an important role in the regulation of apoptosis in various physiological systems. The receptors are activated by a family of cytokines that include TNF, FasL, and TRAIL. They are characterized by a highly conserved extracellular region containing cysteine-rich repeats and a conserved intracellular region of about 80 amino acids termed the death domain (DD). The DD is important for transducing the death signal by recruiting other DD containing adaptor proteins (FADD, TRADD, RIP) to the death-inducing signaling complex (DISC), resulting in activation of caspases. DR3/WSL-1/Apo-3/TRAMP/LARD is a TNFR family member containing the characteristic extracellular cysteine-repeats, transmembrane region, and an intracellular DD. DR3 is activated by its ligand Apo-3L/TWEAK to induce apoptosis and activation of NF-kappaB. Like TNF-R1, DR3 binds to the DD adaptor protein TRADD, which can then associate with other DD proteins like FADD and RIP as well as members of the TRAF family. Tissue expression of DR3 is very restricted, primarily seen on the surface of activated thymocytes and lymphocytes and plays an important role in thymocyte negative selection. Studies have also indicated an association with DR3 and rheumatoid arthritis.

NCBI and Uniprot Product Information

• NCBI GI #: 23200025
• NCBI GeneID: 8718
• NCBI Accession #: NP_683868.1
• NCBI GenBank Nucleotide #: NP_683868.1
• UniProt Accession #: Q93038
• Molecular Weight: ~ 55kDa
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 25 isoform 4
• NCBI Official Synonym Full Names: TNF receptor superfamily member 25
• NCBI Official Symbol: TNFRSF25
• NCBI Official Synonym Symbols: DR3; TR3; DDR3; LARD; APO-3; TRAMP; WSL-1; GEF720; WSL-LR; PLEKHG5; TNFRSF12
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 25
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 25
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF25
• UniProt Synonym Gene Names: APO3; DDR3; DR3; TNFRSF12; WSL; WSL1; LARD
• UniProt Entry Name: TNR25_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed preferentially in the tissues enriched in lymphocytes, and it may play a role in regulating lymphocyte homeostasis. This receptor has been shown to stimulate NF-kappa B activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domain containing adaptor proteins. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFRSF25: Receptor for TNFSF12/APO3L/TWEAK. Interacts directly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis. Homodimer. Interacts strongly via the death domains with TNFRSF1 and TRADD to activate at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. Interacts with BAG4. Abundantly expressed in thymocytes and lymphocytes. Detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. Also found in the prostate. 12 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Apoptosis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: integral to plasma membrane; plasma membrane; extracellular region; cytosol

Molecular Function: tumor necrosis factor receptor activity; receptor activity

Biological Process: regulation of apoptosis; cell surface receptor linked signal transduction; tumor necrosis factor-mediated signaling pathway; apoptosis; signal transduction