Western Blot (WB)
TNF alpha Polyclonal Antibody | anti-TNF alpha antibody
TNF alpha Rabbit Polyclonal Antibody
IHC: 1:100-200
Western Blot (WB)
Western Blot (WB)
Western Blot (WB)
(Western blot analysis of LPS Treated HepG2 with TNF alpha Rabbit pAb E12-447 diluted at 1: 2, 000.Western blot analysis of Recombinant Human TNF a Protein with TNF alpha Rabbit pAb E12-447 diluted at 1: 2, 000.)
Western Blot (WB)
(Western blot analysis of LPS Treated HepG2 with TNF alpha Rabbit pAb E12-447 diluted at 1: 2, 000.Western blot analysis of Recombinant Human TNF a Protein with TNF alpha Rabbit pAb E12-447 diluted at 1: 2, 000.)
Immunohistochemisry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma using TNF a (E12-447)Rabbit pAb diluted at 1: 200.)
Immunohistochemisry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma using TNF a (E12-447)Rabbit pAb diluted at 1: 200.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
Uniprot Description
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918).
Research Articles on TNF alpha
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Product Notes
The TNF alpha tnf (Catalog #AAA8510469) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The TNF alpha Rabbit Polyclonal Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TNF alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1,000-2,000 IHC: 1:100-200. Researchers should empirically determine the suitability of the TNF alpha tnf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNF alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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