Western Blot (WB)
(Western blot analysis of extracts from HUVEC cells treated with PMA using TNAP1 antibody.)
Rabbit TNAP1 Polyclonal Antibody | anti-TNAP1 antibody
TNAP1 Antibody
Gene Names
TNFAIP1; B12; B61; EDP1; BTBD34; hBACURD2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
TNAP1; Polyclonal Antibody; TNAP1 Antibody; alpha-induced protein 1; BACD2_HUMAN; BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2; BTB/POZ domain-containing protein TNFAIP1; endothelial; hBACURD2; Protein B12; tnfaip1; Tumor necrosis factor alpha induced protein 1 (endothelial); Tumor necrosis factor alpha induced protein 1; Tumor necrosis factor; anti-TNAP1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
TNAP1 antibody detects endogenous levels of total TNAP1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
316
Applicable Applications for anti-TNAP1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Cytoplasm. Nucleus. Endosome. Colocalizes with RHOB in endosomes.
Tissue Specificity
By TNF, IL1B/interleukin-1 beta and bacterial lipopolysaccharides (LPS).
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (90%), Bovine (90%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (80%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from HUVEC cells treated with PMA using TNAP1 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HUVEC cells treated with PMA using TNAP1 antibody.)
Immunofluorescene (IF)
(MBS9604733 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9604733 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-TNAP1 antibody
Function: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(TNFAIP1) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Its interaction with RHOB may regulate apoptosis. May enhance the PCNA-dependent DNA polymerase delta activity.
Subunit Structure: Component of the BCR(TNFAIP1) E3 ubiquitin ligase complex, at least composed of CUL3, TNFAIP1/BACURD2 and RBX1. Interacts with RHOA; with a preference for RhoA-GDP. Interacts with RHOB. Interacts with PCNA. Interacts with CSNK2B.
Post-translational Modifications: Phosphorylation at Ser-280 by CK2 facilitates the nucleus localization and increases interaction with PCNA.
Similarity: Belongs to the BACURD family.
Subunit Structure: Component of the BCR(TNFAIP1) E3 ubiquitin ligase complex, at least composed of CUL3, TNFAIP1/BACURD2 and RBX1. Interacts with RHOA; with a preference for RhoA-GDP. Interacts with RHOB. Interacts with PCNA. Interacts with CSNK2B.
Post-translational Modifications: Phosphorylation at Ser-280 by CK2 facilitates the nucleus localization and increases interaction with PCNA.
Similarity: Belongs to the BACURD family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 34 kD
Predicted: 37 kDa
Predicted: 37 kDa
NCBI Official Full Name
BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
NCBI Official Synonym Full Names
TNF alpha induced protein 1
NCBI Official Symbol
TNFAIP1
NCBI Official Synonym Symbols
B12; B61; EDP1; BTBD34; hBACURD2
NCBI Protein Information
BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
UniProt Protein Name
BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
UniProt Gene Name
TNFAIP1
UniProt Synonym Gene Names
BACURD2; EDP1; hBACURD2
NCBI Description
This gene was identified as a gene whose expression can be induced by the tumor necrosis factor alpha (TNF) in umbilical vein endothelial cells. Studies of a similar gene in mouse suggest that the expression of this gene is developmentally regulated in a tissue-specific manner. [provided by RefSeq, Jul 2008]
Uniprot Description
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(TNFAIP1) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Its interaction with RHOB may regulate apoptosis. May enhance the PCNA-dependent DNA polymerase delta activity.
Research Articles on TNAP1
Similar Products
Product Notes
The TNAP1 tnfaip1 (Catalog #AAA9604733) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TNAP1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TNAP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the TNAP1 tnfaip1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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