Goat TMPRSS3 Polyclonal Antibody | anti-TMPRSS3 antibody

Goat anti-TMPRSS3 Antibody

Cat. Num. AAA421245

• Gene Names: TMPRSS3; DFNB8; DFNB10; ECHOS1; TADG12
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: TMPRSS3; Polyclonal Antibody; Goat anti-TMPRSS3 Antibody; transmembrane protease; serine 3; DFNB10; DFNB8; ECHOS1; TADG12; serine protease TADG12; serine 3 antibody; DFNB10 antibody; DFNB8 antibody; ECHOS1 antibody; TADG12 antibody; serine protease TADG12 antibody; anti-TMPRSS3 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: EKIVYHSKYKPKR
• Sequence Length: 454

• Applicable Applications for anti-TMPRSS3 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Western Blot: Approx 48kDa band observed in A431 lysates (calculated MW of 49.4kDa according to NP_076927.1). Recommended concentration: 0.1-0.3ug/ml.
• Immunogen: Peptide with sequence C-EKIVYHSKYKPKR, from the internal region of the protein sequence according to NP_076927.1; NP_115777.1; NP_115780.1; NP_115781.1.
• Epitope: Internal region
• Note: This antibody is expected to recognise all four reported isoforms (NP_076927.1; NP_115777.1; NP_115780.1; NP_115781.1)
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.1ug/ml) staining of A431 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 13173471
• NCBI GeneID: 64699
• NCBI Accession #: NP_076927.1; NP_115777.1; NP_115780.1; NP_115781.1
• NCBI GenBank Nucleotide #: NM_024022.2
• Molecular Weight: 58,324 Da
• NCBI Official Full Name: transmembrane protease serine 3 isoform 1
• NCBI Official Synonym Full Names: transmembrane protease, serine 3
• NCBI Official Symbol: TMPRSS3
• NCBI Official Synonym Symbols: DFNB8; DFNB10; ECHOS1; TADG12
• NCBI Protein Information: transmembrane protease serine 3
• UniProt Protein Name: Transmembrane protease serine 3
• Protein Family: Transmembrane protease serine
• UniProt Gene Name: TMPRSS3
• UniProt Synonym Gene Names: ECHOS1; TADG12
• UniProt Entry Name: TMPS3_HUMAN

NCBI Description

This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a serine protease domain, a transmembrane domain, an LDL receptor-like domain, and a scavenger receptor cysteine-rich domain. Serine proteases are known to be involved in a variety of biological processes, whose malfunction often leads to human diseases and disorders. This gene was identified by its association with both congenital and childhood onset autosomal recessive deafness. This gene is expressed in fetal cochlea and many other tissues, and is thought to be involved in the development and maintenance of the inner ear or the contents of the perilymph and endolymph. This gene was also identified as a tumor-associated gene that is overexpressed in ovarian tumors. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2012]

Uniprot Description

TMPRSS3: Probable serine protease that play a role in hearing. Acts as a permissive factor for cochlear hair cells survival and activation at the onset of hearing and is required for saccular hair cell survival. Activates ENaC (in vitro). Defects in TMPRSS3 are the cause of deafness autosomal recessive type 8 (DFNB8). DFNA8 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Defects in TMPRSS3 are the cause of deafness autosomal recessive type 10 (DFNB10). Belongs to the peptidase S1 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Endoplasmic reticulum; Membrane protein, integral; Protease; EC 3.4.21.-

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cell soma; endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane

Molecular Function: scavenger receptor activity; serine-type endopeptidase activity; sodium channel regulator activity

Biological Process: cellular sodium ion homeostasis; proteolysis; receptor-mediated endocytosis; sensory perception of sound

Disease: Deafness, Autosomal Recessive 8