Rabbit TMPRSS15 Polyclonal Antibody | anti-TMPRSS15 antibody

TMPRSS15 Polyclonal Antibody

Cat. Num. AAA3007026

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TMPRSS15; Polyclonal Antibody; TMPRSS15 Polyclonal Antibody; Enteropeptidase; Enterokinase; Serine protease 7; Transmembrane protease serine 15; ENTK; PRSS7; anti-TMPRSS15 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TMPRSS15 polyclonal antibody detects endogenous levels of TMPRSS15 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1019

• Applicable Applications for anti-TMPRSS15 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human TMPRSS15
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of TMPRSS15 polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysateLane2:Raw264. 7 whole cell lysate Lane3:PC12 whole cell lysate)

Related Product Information for anti-TMPRSS15 antibody

Enterokinase, also known as enteropeptidase or serine protease 7, belongs to the peptidase S1 family and localizes to the intestinal brush border in the proximal small intestine. It exists as a heterodimer of a catalytic light chain (LC) and a non-catalytic heavy chain (HC) linked together by a disulfide bond. Enterokinase HC plays a role in macromolecular substrate recognition and specificity. Duodenase is the serine protease responsible for the release and activation of Enterokinase from its inactive precursor. Active Enterokinase recognizes the target sequence, Asp-Asp-Asp-Asp-Lys, and is responsible for catalyzing the conversion of pancreatic trypsinogen to activated trypsin. Activated trypsin then further activates digestive enzymes such as chymotrypsin, carboxypeptidases, elastases and lipases, releasing them from their inactive precursors. Enterokinase is important for proper digestion of proteins. Improper functioning of Enterokinase may result in congenital enteropeptidase deficiency. This recessively inherited disorder leads to severe protein malabsorption and can result in low serum protein, chronic diarrhea and, in infants, a failure to thrive.

NCBI and Uniprot Product Information

• NCBI GI #: 223942069
• NCBI Accession #: NP_002763.2
• NCBI GenBank Nucleotide #: NP_002763.2
• UniProt Accession #: P98073
• Molecular Weight: ~ 113kDa
• NCBI Official Full Name: enteropeptidase
• UniProt Protein Name: Enteropeptidase
• Protein Family: Enteropeptidase
• UniProt Gene Name: TMPRSS15
• UniProt Synonym Gene Names: ENTK; PRSS7
• UniProt Entry Name: ENTK_HUMAN

Uniprot Description

PRSS7: Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases. Defects in TMPRSS15 are a cause of enterokinase deficiency (ENTKD); a life-threatening intestinal malabsorption disorder characterized by diarrhea and failure to thrive. Belongs to the peptidase S1 family.

Protein type: EC 3.4.21.9; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 21q21.1

Cellular Component: brush border

Molecular Function: protein binding

Disease: Enterokinase Deficiency