Highly validated and characterized monoclonal/polyclonal
antibodies and recombinant
proteins
The majority of AAA Biotech’s antibodies are highly validated and can be use in multiple
applications such as ELISA, FC,
ICC, IF, IHC, IP, WB, etc. We have antibodies available for rare species, in multiple conjugated
forms or recombinant
antibodies.
As for our high quality proteins, the majority have 90% purity, detected by SDS-PAGE while some are
available in
different tags such as Flag, GST, His, MBP, etc. We also carry high quality native and biologically
active proteins.
AAA Biotech is constantly working to expand our capacity to provide recombinant proteins and
antibodies to most
target proteins.
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IHC (Immunohistochemistry)||Basigin/Emmprin/CD147 Antibody-Immunohistochemistry of paraffin-embedded Human esophageal tissue.||AAA21323_IHC7.jpg!!IHC (Immunohistchemistry)||Basigin/Emmprin/CD147 Antibody-Immunohistochemistry of paraffin-embedded human stomach cancer tissue.||AAA21323_IHC6.jpg!!IHC (Immunohistochemistry)||Basigin/Emmprin/CD147 Antibody-Immunohistochemistry of paraffin-embedded Human liver injury tissue.||AAA21323_IHC5.jpg!!IHC (Immunohistochemistry)||Basigin/Emmprin/CD147 Antibody-Immunohistochemistry of paraffin-embedded Human gastric tissue.||AAA21323_IHC4.jpg!!IHC (Immunohistochemistry)||Basigin/Emmprin/CD147 Antibody-Human Brain, Cortex, Capillaries: Formalin-Fixed, Paraffin-Embedded (FFPE)||AAA21323_IHC3.jpg!!IHC (Immunohistochemistry)||Basigin/Emmprin/CD147 Antibody-Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)||AAA21323_IHC2.jpg!!WB (Western Blot)||Basigin/Emmprin/CD147 Antibody-Western blot analysis of extracts of mouse heart, using BSG antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.||AAA21323_WB.jpg
⇄⧉etc_term1 => string (359) "Target||Human Basigin/Emmprin/CD147!!Immunogen||Recombinant fusion protein c...
$value['etc_term1']
Target||Human Basigin/Emmprin/CD147!!Immunogen||Recombinant fusion protein containing a sequence corresponding to amino acids 30-200 of human BSG (NP_940991.1). VEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQKTEFKVDSDDQWGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESVPPVTDWAWYKITDSEDKALMNGSESRFFVSSSQGRSELHIENLNMEADPGQYRCNGTSSKGSDQAIITL!!Conjugation||Unconjugated
⇄⧉products_description => string (208) "CD147 antibody is an unconjugated rabbit polyclonal antibody to CD147 (Basig...
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CD147 antibody is an unconjugated rabbit polyclonal antibody to CD147 (Basigin/Emmprin) from human. It is reactive with human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
IHC (Immunohistochemistry)||Basigin/Emmprin/CD147 Antibody-Immunohistochemistry of paraffin-embedded Human esophageal tissue.||AAA21323_IHC7.jpg!!IHC (Immunohistchemistry)||Basigin/Emmprin/CD147 Antibody-Immunohistochemistry of paraffin-embedded human stomach cancer tissue.||AAA21323_IHC6.jpg!!IHC (Immunohistochemistry)||Basigin/Emmprin/CD147 Antibody-Immunohistochemistry of paraffin-embedded Human liver injury tissue.||AAA21323_IHC5.jpg!!IHC (Immunohistochemistry)||Basigin/Emmprin/CD147 Antibody-Immunohistochemistry of paraffin-embedded Human gastric tissue.||AAA21323_IHC4.jpg!!IHC (Immunohistochemistry)||Basigin/Emmprin/CD147 Antibody-Human Brain, Cortex, Capillaries: Formalin-Fixed, Paraffin-Embedded (FFPE)||AAA21323_IHC3.jpg!!IHC (Immunohistochemistry)||Basigin/Emmprin/CD147 Antibody-Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)||AAA21323_IHC2.jpg!!WB (Western Blot)||Basigin/Emmprin/CD147 Antibody-Western blot analysis of extracts of mouse heart, using BSG antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.||AAA21323_WB.jpg
⇄⧉etc_term1 => string (359) "Target||Human Basigin/Emmprin/CD147!!Immunogen||Recombinant fusion protein c...
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Target||Human Basigin/Emmprin/CD147!!Immunogen||Recombinant fusion protein containing a sequence corresponding to amino acids 30-200 of human BSG (NP_940991.1). VEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQKTEFKVDSDDQWGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESVPPVTDWAWYKITDSEDKALMNGSESRFFVSSSQGRSELHIENLNMEADPGQYRCNGTSSKGSDQAIITL!!Conjugation||Unconjugated
⇄⧉products_description => string (208) "CD147 antibody is an unconjugated rabbit polyclonal antibody to CD147 (Basig...
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CD147 antibody is an unconjugated rabbit polyclonal antibody to CD147 (Basigin/Emmprin) from human. It is reactive with human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
IHC (Immunohistochemistry)||Basigin/Emmprin/CD147 Antibody-Immunohistochemistry of paraffin-embedded Human esophageal tissue.||AAA21323_IHC7.jpg!!IHC (Immunohistchemistry)||Basigin/Emmprin/CD147 Antibody-Immunohistochemistry of paraffin-embedded human stomach cancer tissue.||AAA21323_IHC6.jpg!!IHC (Immunohistochemistry)||Basigin/Emmprin/CD147 Antibody-Immunohistochemistry of paraffin-embedded Human liver injury tissue.||AAA21323_IHC5.jpg!!IHC (Immunohistochemistry)||Basigin/Emmprin/CD147 Antibody-Immunohistochemistry of paraffin-embedded Human gastric tissue.||AAA21323_IHC4.jpg!!IHC (Immunohistochemistry)||Basigin/Emmprin/CD147 Antibody-Human Brain, Cortex, Capillaries: Formalin-Fixed, Paraffin-Embedded (FFPE)||AAA21323_IHC3.jpg!!IHC (Immunohistochemistry)||Basigin/Emmprin/CD147 Antibody-Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)||AAA21323_IHC2.jpg!!WB (Western Blot)||Basigin/Emmprin/CD147 Antibody-Western blot analysis of extracts of mouse heart, using BSG antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.||AAA21323_WB.jpg
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Target||Human Basigin/Emmprin/CD147!!Immunogen||Recombinant fusion protein containing a sequence corresponding to amino acids 30-200 of human BSG (NP_940991.1). VEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQKTEFKVDSDDQWGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESVPPVTDWAWYKITDSEDKALMNGSESRFFVSSSQGRSELHIENLNMEADPGQYRCNGTSSKGSDQAIITL!!Conjugation||Unconjugated
⇄⧉products_description => string (208) "CD147 antibody is an unconjugated rabbit polyclonal antibody to CD147 (Basig...
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CD147 antibody is an unconjugated rabbit polyclonal antibody to CD147 (Basigin/Emmprin) from human. It is reactive with human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Assay Type||Quantitative Sandwich!!Samples||Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids!!Detection Range||0.2-70ng/ml!!Sensitivity||0.092ng/ml
⇄⧉etc_term2 => string (403) "Intra-assay Precision||Intra-Assay Precision (Precision within an assay) Thr...
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Intra-assay Precision||Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%!!Inter-assay Precision||Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
⇄⧉products_description => string (885) "Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (EL...
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Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Fish ACE antibody. ACE present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Fish ACE Antibody is added and binds to ACE in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ACE antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Fish ACE. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.<br><br>Intended Uses: This sandwich kit is for the accurate quantitative detection of Fish Angiotensin converting enzyme (also known as ACE) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
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aaa11311 fish typical testing data standard curve for reference only aaa11311_sc elisa kit angiotensin converting enzyme ace isoform b ance anon est:fe3d10 bg:ds08220.3 br31 cg8827 dmelcg8827 l 2 34eb l34eb race 70,914 da dipeptidyl carboxypeptidase i kininase ii 24584232 np_723852.1 q10714 nm_165070.3 q27572 q9nke4 q9tx66 q9vjv3 a4v0p3 samples serum plasma cell culture supernates lysates tissue homogenates assay type quantitative sandwich detection range 0.2ng ml 70ng sensitivity 0.092ng intra cv<8 inter cv<10 l2
⇄⧉specificity => string (238) "This assay has high sensitivity and excellent specificity for detection of A...
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This assay has high sensitivity and excellent specificity for detection of Angiotensin I Converting Enzyme (ACE).<br>No significant cross-reactivity or interference between Angiotensin I Converting Enzyme (ACE) and analogues was observed.
⇄purity => string (3) "N/A"
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⇄⧉storage_stability => string (475) "The stability of kit is determined by the loss rate of activity. The loss ra...
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. <br>To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay Type||Double-antibody Sandwich!!Samples||Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids!!Detection Range||0.78-50ng/mL!!Sensitivity||0.28ng/mL
⇄⧉etc_term2 => string (508) "Application||Enzyme-linked immunosorbent assay for Antigen Detection.!!Intra...
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Application||Enzyme-linked immunosorbent assay for Antigen Detection.!!Intra-assay Precision (Precision within an assay)||3 samples with low, middle and high level Angiotensin I Converting Enzyme (ACE) were tested 20 times on one plate, respectively.!!Inter-assay Precision (Precision between assays)||3 samples with low, middle and high level Angiotensin I Converting Enzyme (ACE) were tested on 3 different plates, 8 replicates in each plate.!!CV(%) =||SD/meanX100!!Intra-Assay||CV<10%!!Inter-Assay||CV<12%
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⇄products_name => string (37) "Angiotensin I Converting Enzyme (ACE)"
⇄⧉products_description => string (999) "The test principle applied in this kit is Sandwich enzyme immunoassay. The m...
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin I Converting Enzyme (ACE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiotensin I Converting Enzyme (ACE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiotensin I Converting Enzyme (ACE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiotensin I Converting Enzyme (ACE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
⇄⧉search_terms => string (926) "aaa20522 human this assay has high sensitivity and excellent specificity for...
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aaa20522 human this assay has high sensitivity and excellent specificity for detection of angiotensin i converting enzyme ace no significant cross reactivity or interference between analogues was observed typical standard curve testing data aaa20522_td elisa kit cd143 ace1 dcp1 acei kininase ii peptidyl dipeptidase a dipeptidyl carboxypeptidase 1 soluble form partial dcp ich mvcd3 78,694 da cd_antigen ace_human 76057114 aba39229.1 p12821 p22966 q53yx9 q59gy8 q7m4l4 b0lpf0 b4dxi3 e7eu16 106180 kinase cardiovascular biology type double antibody sandwich samples serum plasma tissue homogenates cell lysates culture supernates other biological fluids range 0.39 25ng ml < 0.16ng application linked immunosorbent antigen intra precision within an 3 with low middle level were tested 20 times on one plate respectively inter assays different plates 8 replicates in each cv = sd meanx100 carboxypeptidase1 an3 tested20 plates8
⇄⧉products_name_syn => string (124) "Mouse Angiotensin II Converting Enzyme ELISA Kit; Angiotensin II Converting ...
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Mouse Angiotensin II Converting Enzyme ELISA Kit; Angiotensin II Converting Enzyme; Angiotensin II Converting Enzyme (Mouse)
⇄products_gene_name => string (6) "ACE II"
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⇄products_gene_name_syn => string (3) "N/A"
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⇄⧉products_description => string (1455) "Intended Uses: This ACE2 ELISA kit is a 1.5 hour solid-phase ELISA designed ...
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Intended Uses: This ACE2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ACE2. This ELISA kit for research use only!<br><br>Principle of the Assay: ACE2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ACE2. Standards or samples are then added to the microtiter plate wells and ACE2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ACE2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ACE2 are added to each well to "sandwich" the ACE2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ACE2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACE2 concentration in each sample is interpolated from this standard curve.
ACE Inhibitor Pathway||198763!!Protein Digestion And Absorption Pathway||172847!!Protein Digestion And Absorption Pathway||171868!!Renin-angiotensin System Pathway||83075!!Renin-angiotensin System Pathway||486
⇄⧉search_terms => string (500) "aaa16254 mouse typical testing data standard curve for reference only aaa162...
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aaa16254 mouse typical testing data standard curve for reference only aaa16254_td elisa kit angiotensin ii converting enzyme ace 2 i peptidyl dipeptidase a ace2 aceh dkfzp434a014 otthump00000022963 metalloprotease mprot15 related carboxypeptidase homolog 92,463 da ace2_human 11225609 np_068576.1 q9byf1 nm_021804.2 q6uwp0 q86wt0 q9nra7 q9ufz6 300335 samples serum plasma cell culture supernatants body fluid and tissue homogenate assay type quantitative sandwich sensitivity 1.0 pg ml sensitivity1.0
⇄⧉specificity => string (175) "This assay has high sensitivity and excellent specificity for detection of r...
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This assay has high sensitivity and excellent specificity for detection of rat ACE. No significant cross-reactivity or interference between rat ACE and analogues was observed.
⇄purity => string (3) "N/A"
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⇄form => string (3) "N/A"
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⇄concentration => string (3) "N/A"
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⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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⇄products_name => string (58) "angiotensin I converting enzyme (peptidyl-dipeptidase A) 1"
Rat Angiotensin converting enzyme; ACE ELISA Kit; ACE1; CD143; DCP; DCP1; MGC26566; MVCD3; CD143 antigen; angiotensin I converting enzyme 1; angiotensin converting enzyme; somatic isoform; carboxycathepsin; dipeptidyl carboxypeptidase 1; kininase II; pep; angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
⇄products_gene_name => string (3) "ACE"
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⇄products_gene_name_syn => string (3) "N/A"
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⇄⧉products_description => string (616) "Principle of the Assay: This assay employs the competitive inhibition enzyme...
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Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ACE. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for ACE. The competitive inhibition reaction is launched between with pre-coated ACE and ACE in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of ACE in the samples. The color development is stopped and the intensity of the color is measured.
Dipeptidyl carboxypeptidase I; Kininase II; CD_antigen: CD143Cleaved into the following chain:Angiotensin-converting enzyme, soluble form
⇄sp_gene_name => string (3) "ACE"
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⇄sp_gene_name_syn => string (9) "DCP1; ACE"
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⇄sp_entry_name => string (9) "ACE_RABIT"
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⇄sp_interactions => string (8) "FLNA||12"
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⇄⧉search_terms => string (779) "aaa15408 rat this assay has high sensitivity and excellent specificity for d...
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aaa15408 rat this assay has high sensitivity and excellent specificity for detection of ace no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15408_td elisa kit angiotensin i converting enzyme peptidyl dipeptidase a 1 ace1 cd143 dcp dcp1 mgc26566 mvcd3 antigen somatic isoform carboxycathepsin dipeptidyl carboxypeptidase kininase ii pep 150,406 da cd_antigen cd143cleaved into the following chain:angiotensin soluble form ace_rabit 126723380 np_001075864.1 p12822 nm_001082395.1 o02852 p22968 samples serum plasma tissue homogenates range 78 pg ml 5000 <19.5 intra precision within an cv <8 three known concentration were tested twenty times on one plate to assess inter assays <10 in a1 range78
⇄⧉specificity => string (376) "This assay has high sensitivity and excellent specificity for detection of A...
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This assay has high sensitivity and excellent specificity for detection of ACE2. No significant cross-reactivity or interference between ACE2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACE2 and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
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⇄storage_stability => string (34) "Store all reagents at 2-8 degree C"
⇄⧉products_name_syn => string (124) "Human Angiotensin II Converting Enzyme ELISA Kit; Angiotensin II Converting ...
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Human Angiotensin II Converting Enzyme ELISA Kit; Angiotensin II Converting Enzyme; Angiotensin II Converting Enzyme (Human)
⇄products_gene_name => string (6) "ACE II"
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⇄⧉products_description => string (1497) "Intended Uses: This ACE2 ELISA kit is a 1.5 hour solid-phase ELISA designed ...
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Intended Uses: This ACE2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ACE2. This ELISA kit for research use only, not for therapeutic or test applications!<br><br>Principle of the Assay: ACE2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ACE2. Standards or samples are then added to the microtiter plate wells and ACE2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ACE2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ACE2 are added to each well to "sandwich" the ACE2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ACE2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACE2 concentration in each sample is interpolated from this standard curve.
ACE Inhibitor Pathway||198763!!Protein Digestion And Absorption Pathway||172847!!Protein Digestion And Absorption Pathway||171868!!Renin-angiotensin System Pathway||83075!!Renin-angiotensin System Pathway||486
⇄⧉search_terms => string (742) "aaa16136 human this assay has high sensitivity and excellent specificity for...
$value[4]['_source']['search_terms']
aaa16136 human this assay has high sensitivity and excellent specificity for detection of ace2 no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa16136_sc elisa kit angiotensin ii converting enzyme ace 2 i peptidyl dipeptidase a aceh dkfzp434a014 otthump00000022963 metalloprotease mprot15 related carboxypeptidase homolog 92,463 da ace2_human 11225609 np_068576.1 q9byf1 nm_021804.2 q6uwp0 q86wt0 q9nra7 q9ufz6 300335 samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative sandwich 1.0pg ml
⇄⧉specificity => string (376) "This assay has high sensitivity and excellent specificity for detection of A...
$value[5]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of ACE1. No significant cross-reactivity or interference between ACE1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACE1 and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[5]['_source']['purity']
⇄form => string (3) "N/A"
$value[5]['_source']['form']
⇄concentration => string (3) "N/A"
$value[5]['_source']['concentration']
⇄storage_stability => string (34) "Store all reagents at 2-8 degree C"
⇄⧉products_name_syn => string (121) "Human Angiotensin I Converting Enzyme ELISA Kit; Angiotensin I Converting En...
$value[5]['_source']['products_name_syn']
Human Angiotensin I Converting Enzyme ELISA Kit; Angiotensin I Converting Enzyme; Angiotensin I Converting Enzyme (Human)
⇄products_gene_name => string (5) "ACE I"
$value[5]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[5]['_source']['products_gene_name_syn']
⇄⧉products_description => string (1389) "Principle of the Assay: ACE1 ELISA kit applies the competitive enzyme immuno...
$value[5]['_source']['products_description']
Principle of the Assay: ACE1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ACE1 antibody and an ACE1-HRP conjugate. The assay sample and buffer are incubated together with ACE1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACE1 concentration since ACE1 from samples and ACE1-HRP conjugate compete for the anti-ACE1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACE1 from the sample, fewer sites are left to bind ACE1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACE1 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This ACE1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ACE1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉search_terms => string (817) "aaa16351 human this assay has high sensitivity and excellent specificity for...
$value[5]['_source']['search_terms']
aaa16351 human this assay has high sensitivity and excellent specificity for detection of ace1 no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa16351_sc elisa kit angiotensin i converting enzyme ace peptidyl dipeptidase a 1 dcp ich dcp1 cd143 mvcd3 kininase ii peptidase p antigen testicular eca carboxycathepsin dipeptidyl carboxypeptidase somatic isoform transcript 149,715 da ace_human 37790804 aar03504 p12821 p22966 q53yx9 q59gy8 q7m4l4 b0lpf0 b4dxi3 e7eu16 106180 samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 1.0 pg ml a1 competitive1.0
⇄⧉specificity => string (184) "The Human ACE2 ELISA Kit allows for the detection and quantification of endo...
$value[6]['_source']['specificity']
The Human ACE2 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human ACE2 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
⇄purity => string (3) "N/A"
$value[6]['_source']['purity']
⇄form => string (3) "N/A"
$value[6]['_source']['form']
⇄concentration => string (3) "N/A"
$value[6]['_source']['concentration']
⇄⧉storage_stability => string (107) "Shipped and store at 4 degree C for 6 months, store at -20 degree C for one ...
$value[6]['_source']['storage_stability']
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
⇄⧉products_description => string (1610) "Background: AngiotensinI-converting enzyme 2 (ACE 2) is a protein belongs to...
$value[6]['_source']['products_description']
Background: AngiotensinI-converting enzyme 2 (ACE 2) is a protein belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. By sequence similarity to a sequence in GenBank, this gene is mapped to Xp22.2. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.<br><br>Principle of the Assay: The Human ACE2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human ACE2 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human ACE2 coated on a 96-well plate. Standards and samples are pipetted into the wells and ACE2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human ACE2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ACE2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
angiotensin-converting enzyme 2; peptidyl-dipeptidase A; metalloprotease MPROT15; ACE-related carboxypeptidase; angiotensin-converting enzyme homolog; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
⇄ncbi_chrom_loc => string (3) "N/A"
$value[6]['_source']['ncbi_chrom_loc']
⇄ncbi_gene_id => string (5) "59272"
$value[6]['_source']['ncbi_gene_id']
⇄ncbi_mol_weight => string (9) "63,912 Da"
$value[6]['_source']['ncbi_mol_weight']
⇄⧉ncbi_pathways => string (356) "ACE Inhibitor Pathway||198763!!Metabolism Of Angiotensinogen To Angiotensins...
$value[6]['_source']['ncbi_pathways']
ACE Inhibitor Pathway||198763!!Metabolism Of Angiotensinogen To Angiotensins Pathway||685555!!Metabolism Of Proteins Pathway||106230!!Peptide Hormone Metabolism Pathway||771603!!Protein Digestion And Absorption Pathway||172847!!Protein Digestion And Absorption Pathway||171868!!Renin-angiotensin System Pathway||83075!!Renin-angiotensin System Pathway||486
ACE-related carboxypeptidase; Angiotensin-converting enzyme homolog; ACEH; Metalloprotease MPROT15Cleaved into the following chain:Processed angiotensin-converting enzyme 2
⇄⧉search_terms => string (578) "aaa17882 human the ace2 elisa kit allows for detection and quantification of...
$value[6]['_source']['search_terms']
aaa17882 human the ace2 elisa kit allows for detection and quantification of endogenous levels natural or recombinant proteins within range 62.5 pg ml 4000 sandwich se typical testing data standard curve reference only aaa17882_sc angiotensin converting enzyme 2 ace related carboxypeptidase homolog aceh metalloprotease mprot15 i peptidyl dipeptidase a 63,912 da mprot15cleaved into following chain:processed ace2_human 11225609 np_068576.1 q9byf1 nm_021804.2 q6uwp0 q86wt0 q9nra7 q9ufz6 c7ecu1 300335 samples cell culture supernatants serum plasma sensitivity < 10 enzyme2 <10
⇄⧉purity => string (86) "Antigen-specific affinity chromatography followed by Protein A affinity chro...
$value[7]['_source']['purity']
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
⇄⧉form => string (90) "Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300,...
$value[7]['_source']['form']
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
⇄concentration => string (8) "500ug/mL"
$value[7]['_source']['concentration']
⇄⧉storage_stability => string (496) "Storage:<br>Avoid repeated freeze/thaw cycles.<br>Store at 4 degree C for fr...
$value[7]['_source']['storage_stability']
Storage:<br>Avoid repeated freeze/thaw cycles.<br>Store at 4 degree C for frequent use.<br>Aliquot and store at -20 degree C for 24 months.<br><br>Stability Test:<br>The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western blotting: 0.5-2ug/mL<br>Immunohistochemistry: 5-20ug/mL<br>Immunocytochemistry: 5-20ug/mL<br>Optimal working dilutions must be determined by end user.
⇄⧉testing_protocols => string (933) "IHC (Immunohistochemistry)||DAB staining on IHC-P;<br>Samples: Human Breast ...
$value[7]['_source']['testing_protocols']
IHC (Immunohistochemistry)||DAB staining on IHC-P;<br>Samples: Human Breast cancer Tissue;<br>Primary Ab: 10ug/ml Rabbit Anti-Human ACE Antibody<br>Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Immunohistchemistry)||DAB staining on IHC-P;<br>Samples: Human Stomach Tissue;<br>Primary Ab: 10ug/ml Rabbit Anti-Human ACE Antibody<br>Second Ab: 2ug/mL HRP-Linked<br>Caprine Anti-Rabbit IgG Polyclonal Antibody (Immunohistochemistry)||DAB staining on IHC-P; Samples: Human Prostate Gland Tissue.||AAA20954_IHC5.jpg!!IHC (Immunohistochemistry)||DAB staining on IHC-P; Samples: Human Skin Cancer Tissue.||AAA20954_IHC4.jpg!!IHC (Immunohistochemistry)||DAB staining on IHC-P; Samples: Human Rectum Cancer Tissue.||AAA20954_IHC3.jpg!!IHC (Immunohistochemistry)||DAB staining on IHC-P; Samples: Human Liver Tissue||AAA20954_IHC2.jpg!!WB (Western Blot)||Western Blot: Sample: Recombinant ACE, Human.||AAA20954_WB.jpg
⇄⧉search_terms => string (1025) "aaa20954 rabbit human polyclonal antigen specific affinity chromatography fo...
$value[7]['_source']['search_terms']
aaa20954 rabbit human polyclonal antigen specific affinity chromatography followed by protein a supplied as solution form in 0.01m pbs ph7.4 containing 0.05 proclin 300 50 glycerol western blot wb immunocytochemistry icc immunohistochemistry ihc formalin paraffin elisa eia blotting 0.5 2ug ml 5 20ug optimal working dilutions must be determined end user sample recombinant ace aaa20954_wb dab staining on p samples liver tissue aaa20954_ihc rectum cancer aaa20954_ihc2 skin aaa20954_ihc3 prostate gland aaa20954_ihc4 stomach primary ab 10ug anti antibody second hrp linked caprine igg catalog mbs2086047 aaa20954_ihc7 breast aaa20954_ihc8 angiotensin i converting enzyme to isoform 1 dcp ace1 dcp1 cd143 dipeptidyl carboxypeptidase kininase ii cd_antigen 4503273 np_000780.1 p12821 nm_000789.3 p22966 q53yx9 q59gy8 q7m4l4 b0lpf0 b4dxi3 e7eu16 j04144 mrna organism species homo sapiens source preparation traits liquid cross reactivity immunogen ser1160~ser1306 expressed e.coli mbs2029765 proclin300 blotting0.5 ml5 isoform1
⇄⧉specificity => string (169) "This assay has high sensitivity and excellent specificity for detection of A...
$value[8]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of Ace2. No significant cross-reactivity or interference between Ace2 and analogues was observed.
⇄purity => string (3) "N/A"
$value[8]['_source']['purity']
⇄form => string (3) "N/A"
$value[8]['_source']['form']
⇄concentration => string (3) "N/A"
$value[8]['_source']['concentration']
⇄⧉storage_stability => string (156) "Store entire kit at 2-8C for short-term. For longer-term, please store the m...
$value[8]['_source']['storage_stability']
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
⇄⧉products_description => string (825) "Principle of the Assay: This kit was based on sandwich enzyme-linked immune-...
$value[8]['_source']['products_description']
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.
⇄⧉ncbi_pathways => string (359) "ACE Inhibitor Pathway||198763!!Metabolism Of Angiotensinogen To Angiotensins...
$value[8]['_source']['ncbi_pathways']
ACE Inhibitor Pathway||198763!!Metabolism Of Angiotensinogen To Angiotensins Pathway||1268749!!Metabolism Of Proteins Pathway||1268677!!Peptide Hormone Metabolism Pathway||1268746!!Protein Digestion And Absorption Pathway||172847!!Protein Digestion And Absorption Pathway||171868!!Renin-angiotensin System Pathway||83075!!Renin-angiotensin System Pathway||486
⇄⧉search_terms => string (569) "aaa17616 rat this assay has high sensitivity and excellent specificity for d...
$value[8]['_source']['search_terms']
aaa17616 rat this assay has high sensitivity and excellent specificity for detection of ace2 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa17616_sc elisa kit angiotensin converting enzyme 2 ace related carboxypeptidase i aceh 63,912 da homolog ace2_human 11225609 np_068576.1 q9byf1 nm_021804.2 q6uwp0 q86wt0 q9nra7 q9ufz6 c7ecu1 300335 samples serum plasma tissue homogenates other biological fluids type quantitative sandwich range 0.625 40ng ml 0.375ng intra precision cv enzyme2
⇄products_name => string (21) "Angiotensin I (Ang-I)"
$value[9]['_source']['products_name']
⇄products_name_oem => string (37) "Human Angiotensin I (Ang-I) ELISA Kit"
$value[9]['_source']['products_name_oem']
⇄products_name_syn => string (3) "N/A"
$value[9]['_source']['products_name_syn']
⇄products_gene_name => string (5) "Ang-I"
$value[9]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[9]['_source']['products_gene_name_syn']
⇄⧉products_description => string (827) "Principle of the Assay: As mentioned above, this kit utilizes the Double Ant...
$value[9]['_source']['products_description']
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human Ang-I monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
⇄⧉search_terms => string (649) "aaa12606 human no cross reaction with other factors typical testing data sta...
$value[9]['_source']['search_terms']
aaa12606 human no cross reaction with other factors typical testing data standard curve for reference only aaa12606_sc elisa kit angiotensin i ang angiotensinogen preproprotein serpin peptidase inhibitor clade a member 8 agt anhu serpina8 a8 ii pre alpha 1 antiproteinase antitrypsin serine or cysteine proteinase 53,154 da a8cleaved into the following chains:angiotensin 1alternative name s 10 iii iv angt_human 4557287 np_000020.1 p01019 nm_000029.3 q16358 q16359 q96f91 267430 samples serum plasma cell culture supernatant assay type quantitative sandwich detection range 1000 pg ml 15.6 sensitivity up to 5 intra precision member8 alpha1 s10 to5
⇄⧉specificity => string (183) "This assay has high sensitivity and excellent specificity for detection of h...
$value[10]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of human FOLH1. No significant cross-reactivity or interference between human FOLH1 and analogues was observed.
⇄purity => string (3) "N/A"
$value[10]['_source']['purity']
⇄form => string (3) "N/A"
$value[10]['_source']['form']
⇄concentration => string (3) "N/A"
$value[10]['_source']['concentration']
⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
$value[10]['_source']['storage_stability']
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
$value[10]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
⇄⧉products_description => string (735) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
$value[10]['_source']['products_description']
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FOLH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FOLH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FOLH1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FOLH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄⧉search_terms => string (878) "aaa18017 human this assay has high sensitivity and excellent specificity for...
$value[10]['_source']['search_terms']
aaa18017 human this assay has high sensitivity and excellent specificity for detection of folh1 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa18017_td elisa kit folate hydrolase prostate specific membrane antigen 1 glutamate carboxypeptidase 2 fgcp folh gcp2 gcpii naalad1 naaladase psm psma mgcp n acetylated alpha linked acidic dipeptidase cell growth inhibiting protein 27 folylpoly gamma carboxypep isoform i carboxylase ii gene variant f pteroylpoly 84,331 da gig27 folh1_human 62548858 np_001014986.1 q04609 nm_001014986.1 o43748 q16305 q541a4 q8tay3 q9np15 a4uu12 a9cb79 b7z312 b7z343 d3dqs5 e9pdx8 600934 samples serum plasma tissue homogenates type quantitative sandwich range 4.7 ng ml 300 < 1.2 intra precision within an cv antigen1 carboxypeptidase2 protein27 range4.7 ml300 <1.2
⇄⧉specificity => string (173) "This assay has high sensitivity and excellent specificity for detection of A...
$value[11]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of ANG II. No significant cross-reactivity or interference between ANG II and analogues was observed.
⇄purity => string (3) "N/A"
$value[11]['_source']['purity']
⇄form => string (3) "N/A"
$value[11]['_source']['form']
⇄concentration => string (3) "N/A"
$value[11]['_source']['concentration']
⇄⧉storage_stability => string (156) "Store entire kit at 2-8C for short-term. For longer-term, please store the m...
$value[11]['_source']['storage_stability']
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Assay Type||Competitive!!Samples||Serum, plasma, tissue homogenates and other biological fluids!!Detection Range||31.25-2000pg/ml!!Sensitivity||18.75pg/ml
⇄products_name_oem => string (30) "Human Angiotensin II ELISA Kit"
$value[11]['_source']['products_name_oem']
⇄products_name_syn => string (6) "ANG II"
$value[11]['_source']['products_name_syn']
⇄products_gene_name => string (6) "ANG II"
$value[11]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[11]['_source']['products_gene_name_syn']
⇄⧉products_description => string (840) "Principle of the Assay: This kit was based on Competitive-ELISA detection me...
$value[11]['_source']['products_description']
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.
⇄⧉search_terms => string (417) "aaa17627 human this assay has high sensitivity and excellent specificity for...
$value[11]['_source']['search_terms']
aaa17627 human this assay has high sensitivity and excellent specificity for detection of ang ii no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa17627_sc elisa kit angiotensin samples serum plasma tissue homogenates other biological fluids type quantitative competitive range 31.25 2000pg ml 18.75pg intra precision cv<8 inter cv<10
⇄⧉specificity => string (173) "This assay has high sensitivity and excellent specificity for detection of A...
$value[12]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of ANG II. No significant cross-reactivity or interference between ANG II and analogues was observed.
⇄purity => string (3) "N/A"
$value[12]['_source']['purity']
⇄form => string (3) "N/A"
$value[12]['_source']['form']
⇄concentration => string (3) "N/A"
$value[12]['_source']['concentration']
⇄⧉storage_stability => string (156) "Store entire kit at 2-8C for short-term. For longer-term, please store the m...
$value[12]['_source']['storage_stability']
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Assay Type||Competitive!!Samples||Serum, plasma, tissue homogenates and other biological fluids!!Detection Range||31.25-2000pg/ml!!Sensitivity||18.75pg/ml
⇄products_name => string (23) "ANG II (Angiotensin II)"
$value[12]['_source']['products_name']
⇄products_name_oem => string (37) "Rat ANG II (Angiotensin II) ELISA Kit"
$value[12]['_source']['products_name_oem']
⇄products_name_syn => string (3) "N/A"
$value[12]['_source']['products_name_syn']
⇄products_gene_name => string (6) "ANG II"
$value[12]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[12]['_source']['products_gene_name_syn']
⇄⧉products_description => string (840) "Principle of the Assay: This kit was based on Competitive-ELISA detection me...
$value[12]['_source']['products_description']
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.
⇄⧉search_terms => string (245) "aaa27555 rat typical testing data standard curve for reference only aaa27555...
$value[12]['_source']['search_terms']
aaa27555 rat typical testing data standard curve for reference only aaa27555_sc elisa kit ang ii angiotensin samples serum plasma and other biological fluids assay type sandwich double antibody detection range 31.25 2000pg ml sensitivity 18.75pg
⇄⧉specificity => string (185) "This assay has high sensitivity and excellent specificity for detection of m...
$value[13]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of mouse ANG-II. No significant cross-reactivity or interference between mouse ANG-II and analogues was observed.
⇄purity => string (3) "N/A"
$value[13]['_source']['purity']
⇄form => string (3) "N/A"
$value[13]['_source']['form']
⇄concentration => string (3) "N/A"
$value[13]['_source']['concentration']
⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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⇄products_name_oem => string (38) "Mouse angiotensin II, ANG-II ELISA Kit"
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANG-II has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANG-II present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANG-II is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANG-II bound in the initial step. The color development is stopped and the intensity of the color is measured.
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aaa15215 mouse this assay has high sensitivity and excellent specificity for detection of ang ii no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15215_td elisa kit angiotensin samples serum plasma cell culture supernates tissue homogenates type quantitative sandwich range 0.45 pg ml 30 < 0.11 intra precision within an cv <8 three known concentration were tested twenty times on one plate to assess inter assays <10 in ml30
⇄⧉specificity => string (185) "This assay has high sensitivity and excellent specificity for detection of h...
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This assay has high sensitivity and excellent specificity for detection of human ANG-II. No significant cross-reactivity or interference between human ANG-II and analogues was observed.
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANG-II has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANG-II present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANG-II is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANG-II bound in the initial step. The color development is stopped and the intensity of the color is measured.
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aaa15491 human this assay has high sensitivity and excellent specificity for detection of ang ii no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15491_td elisa kit angiotensin samples serum plasma cell culture supernates tissue homogenates type quantitative sandwich range 31.25 pg ml 2000 < 7.8 intra precision within an cv <8 three known concentration were tested twenty times on one plate to assess inter assays <10 in <7.8
⇄⧉specificity => string (171) "This assay has high sensitivity and excellent specificity for detection of A...
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This assay has high sensitivity and excellent specificity for detection of AngII. No significant cross-reactivity or interference between AngII and analogues was observed.
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For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Samples||Rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids!!Assay Type||Quantitative Sandwich!!Detection Range||62.5-4000pg/mL!!Sensitivity||<24.31pg/mL
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AngII were tested 20 times on one plate, respectively. Intra-Assay: CV<10%!!Inter-assay Precision||Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AngII were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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⇄products_name => string (22) "Angiotensin II (AngII)"
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⇄products_name_oem => string (36) "Rat Angiotensin II (AngII) ELISA Kit"
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Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of AngII in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.<br><br>Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AngII. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to AngII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AngII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of AngII in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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aaa13896 rat this assay has high sensitivity and excellent specificity for detection of angii no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa13896_sc elisa kit angiotensin ii ppy pnp pancreatic polypeptide y samples serum plasma tissue homogenates cell lysates culture supernates other biological fluids type quantitative sandwich range 62.5 4000pg ml <24.31pg intra precision within an 3 with low middle level were tested 20 times on one plate respectively cv<10 inter assays different plates 8 replicates in each cv = sd meanx100 cv<12 an3 tested20 plates8
⇄⧉products_description => string (675) "Principle of the Assay: This experiment use double-sandwich elisa technique ...
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Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat ANG II monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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aaa13149 rat no cross reaction with other factors typical testing data standard curve for reference only aaa13149_sc elisa kit angiotensin ang ii partial angt_thets 74765469 q10757.1 samples serum plasma or cell culture supernatant assay type quantitative sandwich detection range 2000 pg ml 31.2 sensitivity up to 12 intra precision <= 8 inter to12 <=8
⇄⧉specificity => string (181) "This assay has high sensitivity and excellent specificity for detection of r...
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This assay has high sensitivity and excellent specificity for detection of rat ANG-II. No significant cross-reactivity or interference between rat ANG-II and analogues was observed.
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANG-II has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANG-II present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANG-II is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANG-II bound in the initial step. The color development is stopped and the intensity of the color is measured.
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aaa15698 rat this assay has high sensitivity and excellent specificity for detection of ang ii no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15698_td elisa kit angiotensin samples serum plasma cell culture supernates tissue homogenates lysates type quantitative sandwich range 4.7 pg ml 300 < 1.17 intra precision within an cv <8 three known concentration were tested twenty times on one plate to assess inter assays <10 in range4.7 ml300
⇄⧉products_description => string (828) "Principle of the Assay: As mentioned above, this kit utilizes the Double Ant...
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Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse ANG II monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
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aaa13052 mouse no cross reaction with other factors typical testing data standard curve for reference only aaa13052_sc elisa kit angiotensin ii ang partial angt_thets 74765469 q10757.1 samples serum plasma or cell culture supernatant assay type quantitative sandwich detection range 2000 pg ml 31.2 sensitivity up to 12 intra precision <= 8 inter to12 <=8
⇄⧉specificity => string (379) "This assay has high sensitivity and excellent specificity for detection of F...
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This assay has high sensitivity and excellent specificity for detection of FOLH1. No significant cross-reactivity or interference between FOLH1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FOLH1 and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
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⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1402) "Principle of the Assay: FOLH1 ELISA kit applies the competitive enzyme immun...
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Principle of the Assay: FOLH1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-FOLH1 antibody and an FOLH1-HRP conjugate. The assay sample and buffer are incubated together with FOLH1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FOLH1 concentration since FOLH1 from samples and FOLH1-HRP conjugate compete for the anti-FOLH1 antibody binding site. Since the number of sites is limited, as more sites are occupied by FOLH1 from the sample, fewer sites are left to bind FOLH1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FOLH1 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This FOLH1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human FOLH1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉search_terms => string (978) "aaa27278 human this assay has high sensitivity and excellent specificity for...
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aaa27278 human this assay has high sensitivity and excellent specificity for detection of folh1 no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa27278_sc elisa kit glutamate carboxypeptidase 2 isoform folate hydrolase prostate specific membrane antigen 1 psm fgcp folh gcp2 psma mgcp gcpii naalad1 naaladase i carboxylase ii cell growth inhibiting gene 27 protein folylpoly gamma variant f pteroylpoly n acetylated alpha linked acidic dipeptidase 50,090 da gig27 folh1_human 62548858 np_001014986.1 q04609 nm_001014986.1 o43748 q16305 q541a4 q8tay3 q9np15 a4uu12 a9cb79 b7z312 b7z343 d3dqs5 e9pdx8 600934 samples serum plasma culture supernatants body fluid tissue homogenate type quantitative competitive 1.0 ng ml carboxypeptidase2 antigen1 gene27 competitive1.0
