Western Blot (WB)
(Western blot analysis of TLR5 in rat brain cell lysate with TLR5 antibody at (A) 0.5ug/ml, (B) 1ug/ml and (C) 2ug/ml.)
Rabbit anti-Human, Mouse TLR5 Polyclonal Antibody
TLR5 (Toll Like Receptor 5, Toll-like Receptor 5 Precursor, TLR 5, TLR-5, FLJ10052, MGC126430, MGC126431, Toll/interleukin 1 Receptor-like Protein 3, TIL3)
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
TLR5; Polyclonal Antibody; TLR5 (Toll Like Receptor 5; Toll-like Receptor 5 Precursor; TLR 5; TLR-5; FLJ10052; MGC126430; MGC126431; Toll/interleukin 1 Receptor-like Protein 3; TIL3); Anti -TLR5 (Toll Like Receptor 5; anti-TLR5 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human TLR5. Species Crossreactivity: mouse.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Applicable Applications for anti-TLR5 antibody
Western Blot (WB)
Application Notes
Dilution: Western Blot: 1-2ug'ml
Immunogen
Synthetic peptide TLR5 16aa near the center of human TLR5 (GenBank accession no. NP_003259).
Positive Control
Rat brain cell lysate
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of TLR5 in rat brain cell lysate with TLR5 antibody at (A) 0.5ug/ml, (B) 1ug/ml and (C) 2ug/ml.)
Western Blot (WB)
(Western blot analysis of TLR5 in rat brain cell lysate with TLR5 antibody at (A) 0.5ug/ml, (B) 1ug/ml and (C) 2ug/ml.)
Related Product Information for anti-TLR5 antibody
Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between
the innate and adaptive immune responses (1,2). The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors (3) so the organism can respond to potential infection. TLR5 recognizes flagellin from both Gram-positive and
Gram-negative bacteria and will cause the activation of NF- B, leading to the activation of TNF- and other cytokines (4). A common TLR5 stop codon polymorphism that disrupts TLR5 signaling is associated with susceptibility to Legionnaires' disease and demonstrates
the importance of TLR5 in the innate immune response (5).
Applications:
Suitable for use in Western Blot. Other applications not tested.
the innate and adaptive immune responses (1,2). The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors (3) so the organism can respond to potential infection. TLR5 recognizes flagellin from both Gram-positive and
Gram-negative bacteria and will cause the activation of NF- B, leading to the activation of TNF- and other cytokines (4). A common TLR5 stop codon polymorphism that disrupts TLR5 signaling is associated with susceptibility to Legionnaires' disease and demonstrates
the importance of TLR5 in the innate immune response (5).
Applications:
Suitable for use in Western Blot. Other applications not tested.
Product Categories/Family for anti-TLR5 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The TLR5 (Catalog #AAA612183) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TLR5 (Toll Like Receptor 5, Toll-like Receptor 5 Precursor, TLR 5, TLR-5, FLJ10052, MGC126430, MGC126431, Toll/interleukin 1 Receptor-like Protein 3, TIL3) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TLR5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Dilution: Western Blot: 1-2ug'ml. Researchers should empirically determine the suitability of the TLR5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TLR5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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