Rabbit TLR1 Polyclonal Antibody | anti-TLR1 antibody

TLR1 Antibody

Cat. Num. AAA9606838

• Gene Names: TLR1; TIL; CD281; rsc786; TIL. LPRS5
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: TLR1; Polyclonal Antibody; TLR1 Antibody; CD281; CD281 antigen; KIAA0012; LPRS5; rsc786; TIL; TIL. LPRS5; Tlr1; TLR1_HUMAN; Toll (Drosophila) homolog; Toll like receptor 1; Toll-like receptor 1; Toll/interleukin-1 receptor-like protein; anti-TLR1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TLR1 antibody detects endogenous levels of total TLR1
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 786

• Applicable Applications for anti-TLR1 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide derived from human TLR1
• Subcellular Location: Cell Membrane. Cytoplasmic Vesicle > Phagosome Membrane.
• Tissue Specificity: Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine.
• Predicted Cross Reactivity: Bovine, Horse, Rabbit, Dog
• Similarity: Bovine (80%), Horse (88%), Rabbit (89%), Dog (90%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts of Rat brain lysate, using TLR1 antibody.)

Immunohistochemistry (IHC)

(MBS9606838 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Related Product Information for anti-TLR1 antibody

Description: Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-3). TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-kappaB and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the TIR domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains including MyD88 (myeloid differentiation factor), MAL/TIRAP (MyD88-adaptor-like/TIR-associated protein), TRIF (Toll-receptor-associated activator of interferon), and TRAM (Toll-receptor-associated molecule). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. Activation of IKK leads to the degradation of IkappaB that normally maintains NF-kappaB inactivity by sequestering it in the cytoplasm. Toll-like receptor expression is highest in peripheral blood leukocytes, monocytes, macrophages, though TLR1 expression may be less restricted than other family members (4, 5). TLR1 associates with TLR2 to cooperatively mediate immune responses to bacterial lipoproteins and lead to NF-kappaB activation (6, 7). TLR1 shows highest homology to TLR6, which shares 69% sequence identity (8).
Function: Participates in the innate immune response to microbial agents. Specifically recognizes diacylated and triacylated lipopeptides. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides (PubMed:21078852). Forms the activation cluster TLR2:TLR1:CD14 in response to triacylated lipopeptides, this cluster triggers signaling from the cell surface and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Subunit Structure: Interacts (via extracellular domain) with TLR2. TLR2 seems to exist in heterodimers with either TLR1 or TLR6 before stimulation by the ligand. The heterodimers form bigger oligomers in response to their corresponding ligands as well as further heterotypic associations with other receptors such as CD14 and/or CD36 (PubMed:16880211, PubMed:17889651). The activation cluster TLR2:TLR1:CD14 forms in response to triacylated lipopeptides (PubMed:16880211). Binds MYD88 (via TIR domain). Interacts with CNPY3 (By similarity).
Similarity: Belongs to the Toll-like receptor family.

NCBI and Uniprot Product Information

• NCBI GI #: 41350337
• NCBI GeneID: 7096
• NCBI Accession #: NP_003254.2
• NCBI GenBank Nucleotide #: NM_003263.3
• UniProt Accession #: Q15399; O15452; Q32MK3; Q32MK4; Q9UG90; D1CS39; D1CS41
• Molecular Weight: Observed: 90 kDa; Predicted: 91 kDa
• NCBI Official Full Name: toll-like receptor 1
• NCBI Official Synonym Full Names: toll like receptor 1
• NCBI Official Symbol: TLR1
• NCBI Official Synonym Symbols: TIL; CD281; rsc786; TIL. LPRS5
• NCBI Protein Information: toll-like receptor 1
• UniProt Protein Name: Toll-like receptor 1
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR1
• UniProt Synonym Gene Names: KIAA0012; TIL

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is ubiquitously expressed, and at higher levels than other TLR genes. Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Participates in the innate immune response to microbial agents. Specifically recognizes diacylated and triacylated lipopeptides. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides (PubMed:21078852). Forms the activation cluster TLR2:TLR1:CD14 in response to triacylated lipopeptides, this cluster triggers signaling from the cell surface and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.